Label-Free Fluorescent Determination of Sunset Yellow in Soft Drinks Based on an Indicator-Displacement Assay
This work reported a fluorescence sensing platform for Sunset Yellow (SY) determination based on competitive host-guest interaction between cucurbit[7]uril (CB7) and signal probe/target molecules. Luteolin/epigallocatechin gallate (EGCG) and SY were selected as the probe and target molecules, respec...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2018/6302345 |
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| _version_ | 1849306324024688640 |
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| author | Shilian Wu Yanqiong Zhang Long Yang Can-Peng Li |
| author_facet | Shilian Wu Yanqiong Zhang Long Yang Can-Peng Li |
| author_sort | Shilian Wu |
| collection | DOAJ |
| description | This work reported a fluorescence sensing platform for Sunset Yellow (SY) determination based on competitive host-guest interaction between cucurbit[7]uril (CB7) and signal probe/target molecules. Luteolin/epigallocatechin gallate (EGCG) and SY were selected as the probe and target molecules, respectively. When luteolin or EGCG entered the CB7 host, its fluorescence significantly improved. However, upon the presence of SY in the performed luteolin·CB7 or EGCG·CB7 complex, this led to a remarkable decrease in fluorescence. This result was due to the fact that the binding constant of CB7/SY (4.9×104 M−1) was greater than that of CB7/luteolin (3.2×103 M−1) or CB7/EGCG (4.8×103 M−1). The fluorescence intensities of CB7/luteolin and CB7/EGCG complexes decreased linearly with increased SY concentration ranges of 0.5–50.0 and 2.0–50.0 μM. The proposed method had detection limits of 0.12 and 0.45 μM and was successfully used to determine SY samples with good recoveries ranging from 96.3% to 103.8%. This competitive mode provided a promising fluorescence assay strategy for potential applications in food safety. |
| format | Article |
| id | doaj-art-2e6494f42e1f42509542ba712eb6cebf |
| institution | Kabale University |
| issn | 0146-9428 1745-4557 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-2e6494f42e1f42509542ba712eb6cebf2025-08-20T03:55:07ZengWileyJournal of Food Quality0146-94281745-45572018-01-01201810.1155/2018/63023456302345Label-Free Fluorescent Determination of Sunset Yellow in Soft Drinks Based on an Indicator-Displacement AssayShilian Wu0Yanqiong Zhang1Long Yang2Can-Peng Li3School of Chemical Science and Technology, Yunnan University, Kunming 650091, ChinaSchool of Chemical Science and Technology, Yunnan University, Kunming 650091, ChinaSchool of Chemical Science and Technology, Yunnan University, Kunming 650091, ChinaSchool of Chemical Science and Technology, Yunnan University, Kunming 650091, ChinaThis work reported a fluorescence sensing platform for Sunset Yellow (SY) determination based on competitive host-guest interaction between cucurbit[7]uril (CB7) and signal probe/target molecules. Luteolin/epigallocatechin gallate (EGCG) and SY were selected as the probe and target molecules, respectively. When luteolin or EGCG entered the CB7 host, its fluorescence significantly improved. However, upon the presence of SY in the performed luteolin·CB7 or EGCG·CB7 complex, this led to a remarkable decrease in fluorescence. This result was due to the fact that the binding constant of CB7/SY (4.9×104 M−1) was greater than that of CB7/luteolin (3.2×103 M−1) or CB7/EGCG (4.8×103 M−1). The fluorescence intensities of CB7/luteolin and CB7/EGCG complexes decreased linearly with increased SY concentration ranges of 0.5–50.0 and 2.0–50.0 μM. The proposed method had detection limits of 0.12 and 0.45 μM and was successfully used to determine SY samples with good recoveries ranging from 96.3% to 103.8%. This competitive mode provided a promising fluorescence assay strategy for potential applications in food safety.http://dx.doi.org/10.1155/2018/6302345 |
| spellingShingle | Shilian Wu Yanqiong Zhang Long Yang Can-Peng Li Label-Free Fluorescent Determination of Sunset Yellow in Soft Drinks Based on an Indicator-Displacement Assay Journal of Food Quality |
| title | Label-Free Fluorescent Determination of Sunset Yellow in Soft Drinks Based on an Indicator-Displacement Assay |
| title_full | Label-Free Fluorescent Determination of Sunset Yellow in Soft Drinks Based on an Indicator-Displacement Assay |
| title_fullStr | Label-Free Fluorescent Determination of Sunset Yellow in Soft Drinks Based on an Indicator-Displacement Assay |
| title_full_unstemmed | Label-Free Fluorescent Determination of Sunset Yellow in Soft Drinks Based on an Indicator-Displacement Assay |
| title_short | Label-Free Fluorescent Determination of Sunset Yellow in Soft Drinks Based on an Indicator-Displacement Assay |
| title_sort | label free fluorescent determination of sunset yellow in soft drinks based on an indicator displacement assay |
| url | http://dx.doi.org/10.1155/2018/6302345 |
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