Features of the immunoreactivity T and B lymphocytes subpopulations and cytokine imbalance in patients with hepatosplenomegaly of different etiology
The aim was to study the mechanisms of immunological dysregulation of cytokine and immunoglobulin production, changes in the CD expression of T and B lymphocyte subpopulations in patients with hepatosplenomegaly of different etiology. Materials and methods. We examined 73 patients with liver cirr...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zaporizhzhia State Medical and Pharmaceutical University
2021-09-01
|
| Series: | Patologìâ |
| Subjects: | |
| Online Access: | http://pat.zsmu.edu.ua/article/view/229454/238837 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850106489677545472 |
|---|---|
| author | O. M. Klimova T. I. Kordon S. V. Sushkov L. A. Drozdova O. V. Lavinska O. S. Merezhko K. O. Bychenko |
| author_facet | O. M. Klimova T. I. Kordon S. V. Sushkov L. A. Drozdova O. V. Lavinska O. S. Merezhko K. O. Bychenko |
| author_sort | O. M. Klimova |
| collection | DOAJ |
| description | The aim was to study the mechanisms of immunological dysregulation of cytokine and immunoglobulin production, changes in the CD expression of T and B lymphocyte subpopulations in patients with hepatosplenomegaly of different etiology.
Materials and methods. We examined 73 patients with liver cirrhosis complicated by portal hypertension, hepatosplenomegaly, and bleeding from phlebectasia. We identified three groups of patients depending on the triggering factors of cirrhosis: the first (I) group – HBV/HCV; the second (II) group – CMV/VEB; the third (III) group – hereditary enzymopathies. The study material was lymphocytes and blood serum. We used the methods of ELISA, immunofluorescence and flow cytometry.
Results. An increase in the concentration of IgA and IgM was revealed against the background of normal number of CD22+ B lymphocytes with HBV/HCV (I group), high level of IgM and their producers, B lymphocytes, with CMV/VEB (II group), in group III with hereditary enzymopathies, the concentration of all immunoglobulins was normal with an increased content of B lymphocytes. Multidirectional changes in the content of cytokines were revealed: in group I the synthesis of anti-inflammatory cytokines IL-4, IL-10 and in group II – pro-inflammatory IL-1β, INF-γ, TNF-α dominated; in group III the concentration of IL-6 and vascular growth factor (VEGF) was maximally increased. The number of activated CD3+CD4+CD25+ T cells was reduced in groups I and II – by 2.3 and 2.0 times respectively, in group III – increased by 1.2 times. The number of regulatory T lymphocytes CD3+CD4+CD25+CD127neg was reduced by half in I and II groups. Expression of co-stimulatory molecules CD3+CD4+CD28+ was low in all groups and the maximum decrease was in group III. In patients with HCV/HBV, an increase in the expression of the late activation marker of lymphocytes CD3+HLA-DR+ by an average of 63 % was noted.
Conclusions. The revealed immune disorders in hepatosplenomegaly of different etiology are characterized by multidirectional changes. Approaches to the treatment of these patients should be complex, taking into account the trigger factors that cause dysregulation of immune responses, which leads to further destruction, and focuses at remodeling target organs. |
| format | Article |
| id | doaj-art-2e4c685d85374150b0260086c1d7e097 |
| institution | OA Journals |
| issn | 2306-8027 2310-1237 |
| language | English |
| publishDate | 2021-09-01 |
| publisher | Zaporizhzhia State Medical and Pharmaceutical University |
| record_format | Article |
| series | Patologìâ |
| spelling | doaj-art-2e4c685d85374150b0260086c1d7e0972025-08-20T02:38:49ZengZaporizhzhia State Medical and Pharmaceutical UniversityPatologìâ2306-80272310-12372021-09-0118217418210.14739/2310-1237.2021.2.229454Features of the immunoreactivity T and B lymphocytes subpopulations and cytokine imbalance in patients with hepatosplenomegaly of different etiologyO. M. Klimova0https://orcid.org/0000-0002-4007-6806T. I. Kordon1https://orcid.org/0000-0001-5813-3656S. V. Sushkov2https://orcid.org/0000-0002-6951-9789L. A. Drozdova3https://orcid.org/0000-0001-9678-4046O. V. Lavinska4https://orcid.org/0000-0001-7320-0925O. S. Merezhko5https://orcid.org/0000-0001-9855-1780K. O. Bychenko6https://orcid.org/0000-0001-7885-7715SI “Zaitsev V. T. Institute of General and Urgent surgery of NAMS of Ukraine”, Kharkiv, UkraineSI “Zaitsev V. T. Institute of General and Urgent Surgery of NAMS of Ukraine”, Kharkiv, UkraineSI “Zaitsev V. T. Institute of General and Urgent Surgery of NAMS of Ukraine”, Kharkiv, UkraineSI “Zaitsev V. T. Institute of General and Urgent Surgery of NAMS of Ukraine”, Kharkiv, UkraineSI “Zaitsev V. T. Institute of General and Urgent Surgery of NAMS of Ukraine”, Kharkiv, UkraineSI “Zaitsev V. T. Institute of General and Urgent Surgery of NAMS of Ukraine”, Kharkiv, UkraineSI “Zaitsev V. T. Institute of General and Urgent surgery of NAMS of Ukraine”, Kharkiv, UkraineThe aim was to study the mechanisms of immunological dysregulation of cytokine and immunoglobulin production, changes in the CD expression of T and B lymphocyte subpopulations in patients with hepatosplenomegaly of different etiology. Materials and methods. We examined 73 patients with liver cirrhosis complicated by portal hypertension, hepatosplenomegaly, and bleeding from phlebectasia. We identified three groups of patients depending on the triggering factors of cirrhosis: the first (I) group – HBV/HCV; the second (II) group – CMV/VEB; the third (III) group – hereditary enzymopathies. The study material was lymphocytes and blood serum. We used the methods of ELISA, immunofluorescence and flow cytometry. Results. An increase in the concentration of IgA and IgM was revealed against the background of normal number of CD22+ B lymphocytes with HBV/HCV (I group), high level of IgM and their producers, B lymphocytes, with CMV/VEB (II group), in group III with hereditary enzymopathies, the concentration of all immunoglobulins was normal with an increased content of B lymphocytes. Multidirectional changes in the content of cytokines were revealed: in group I the synthesis of anti-inflammatory cytokines IL-4, IL-10 and in group II – pro-inflammatory IL-1β, INF-γ, TNF-α dominated; in group III the concentration of IL-6 and vascular growth factor (VEGF) was maximally increased. The number of activated CD3+CD4+CD25+ T cells was reduced in groups I and II – by 2.3 and 2.0 times respectively, in group III – increased by 1.2 times. The number of regulatory T lymphocytes CD3+CD4+CD25+CD127neg was reduced by half in I and II groups. Expression of co-stimulatory molecules CD3+CD4+CD28+ was low in all groups and the maximum decrease was in group III. In patients with HCV/HBV, an increase in the expression of the late activation marker of lymphocytes CD3+HLA-DR+ by an average of 63 % was noted. Conclusions. The revealed immune disorders in hepatosplenomegaly of different etiology are characterized by multidirectional changes. Approaches to the treatment of these patients should be complex, taking into account the trigger factors that cause dysregulation of immune responses, which leads to further destruction, and focuses at remodeling target organs.http://pat.zsmu.edu.ua/article/view/229454/238837hepatosplenomegalytrigger factorimmunoreactivitycytokineslymphocyte subpopulationsb lymphocytest lymphocytes |
| spellingShingle | O. M. Klimova T. I. Kordon S. V. Sushkov L. A. Drozdova O. V. Lavinska O. S. Merezhko K. O. Bychenko Features of the immunoreactivity T and B lymphocytes subpopulations and cytokine imbalance in patients with hepatosplenomegaly of different etiology Patologìâ hepatosplenomegaly trigger factor immunoreactivity cytokines lymphocyte subpopulations b lymphocytes t lymphocytes |
| title | Features of the immunoreactivity T and B lymphocytes subpopulations and cytokine imbalance in patients with hepatosplenomegaly of different etiology |
| title_full | Features of the immunoreactivity T and B lymphocytes subpopulations and cytokine imbalance in patients with hepatosplenomegaly of different etiology |
| title_fullStr | Features of the immunoreactivity T and B lymphocytes subpopulations and cytokine imbalance in patients with hepatosplenomegaly of different etiology |
| title_full_unstemmed | Features of the immunoreactivity T and B lymphocytes subpopulations and cytokine imbalance in patients with hepatosplenomegaly of different etiology |
| title_short | Features of the immunoreactivity T and B lymphocytes subpopulations and cytokine imbalance in patients with hepatosplenomegaly of different etiology |
| title_sort | features of the immunoreactivity t and b lymphocytes subpopulations and cytokine imbalance in patients with hepatosplenomegaly of different etiology |
| topic | hepatosplenomegaly trigger factor immunoreactivity cytokines lymphocyte subpopulations b lymphocytes t lymphocytes |
| url | http://pat.zsmu.edu.ua/article/view/229454/238837 |
| work_keys_str_mv | AT omklimova featuresoftheimmunoreactivitytandblymphocytessubpopulationsandcytokineimbalanceinpatientswithhepatosplenomegalyofdifferentetiology AT tikordon featuresoftheimmunoreactivitytandblymphocytessubpopulationsandcytokineimbalanceinpatientswithhepatosplenomegalyofdifferentetiology AT svsushkov featuresoftheimmunoreactivitytandblymphocytessubpopulationsandcytokineimbalanceinpatientswithhepatosplenomegalyofdifferentetiology AT ladrozdova featuresoftheimmunoreactivitytandblymphocytessubpopulationsandcytokineimbalanceinpatientswithhepatosplenomegalyofdifferentetiology AT ovlavinska featuresoftheimmunoreactivitytandblymphocytessubpopulationsandcytokineimbalanceinpatientswithhepatosplenomegalyofdifferentetiology AT osmerezhko featuresoftheimmunoreactivitytandblymphocytessubpopulationsandcytokineimbalanceinpatientswithhepatosplenomegalyofdifferentetiology AT kobychenko featuresoftheimmunoreactivitytandblymphocytessubpopulationsandcytokineimbalanceinpatientswithhepatosplenomegalyofdifferentetiology |