Molecular characterization of wild and cultivated pigeonpea (Cajanus spp.) species by microsatellites
The primary objective of the current investigation was to use SSR markers to examine the degree of molecular genetic diversity among the 50 cultivated genotypes and five wild species using 17 SSR markers. Seven were monomorphic and 10 markers showed polymorphism. The markers CCB 10, PGM 10, PGM 106...
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Indian Council of Agricultural Research
2025-03-01
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| Series: | The Indian Journal of Agricultural Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/136823 |
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| author | A THANGA HEMAVATHY R KALAIYARASI S KAVITHA N PREMALATHA KAVITHAMANI DURAISAMY NEDUNCHEZHIY VINOTHINI |
| author_facet | A THANGA HEMAVATHY R KALAIYARASI S KAVITHA N PREMALATHA KAVITHAMANI DURAISAMY NEDUNCHEZHIY VINOTHINI |
| author_sort | A THANGA HEMAVATHY |
| collection | DOAJ |
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The primary objective of the current investigation was to use SSR markers to examine the degree of molecular genetic diversity among the 50 cultivated genotypes and five wild species using 17 SSR markers. Seven were monomorphic and 10 markers showed polymorphism. The markers CCB 10, PGM 10, PGM 106, CCM 1538, CCM 1886, CCM 2971, CCM 1263, CCM 0268, CCM 0583, PGM 5 and CCM 1026 showed polymorphism among the genotypes. Microsatellite profiling showed that maximum alleles with amplicon sizes of 150–260 bp. This indicated the effectiveness and higher resolution of such marker systems in detecting molecular diversity. Similar to this, a dendrogram was created using 55 pigeonpea genotypes and highly polymorphic 10 SSR markers which revealed 9 major clusters, ranging from Clusters 1–9, with cluster 6 having the largest number of genotypes (12), and clusters 2, 9 having the smallest number of genotypes (2). This study showed the divergence among pigeonpea genotypes which can be further used in pigeonpea breeding programs. All genotypes involved in this study exhibited a wide range of genetic variability due to different centres of origin, and different genetic constitutions. The genetic relatedness detected in this study may constitute the foundation for future systematic pigeonpea breeding programmes.
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| format | Article |
| id | doaj-art-2e4962bd670a469f846ad506deb39367 |
| institution | Kabale University |
| issn | 0019-5022 2394-3319 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj-art-2e4962bd670a469f846ad506deb393672025-08-20T03:49:45ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192025-03-0195410.56093/ijas.v95i4.136823Molecular characterization of wild and cultivated pigeonpea (Cajanus spp.) species by microsatellitesA THANGA HEMAVATHY0R KALAIYARASI1S KAVITHA2N PREMALATHA3KAVITHAMANI DURAISAMY4NEDUNCHEZHIY VINOTHINI5Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641 003, IndiaCentre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641 003, IndiaCenter for Students Welfare, Tamil Nadu Agricultural University, Coimbatore, Tamil NaduCentre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641 003, IndiaCentre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641 003, IndiaCentre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641 003, India The primary objective of the current investigation was to use SSR markers to examine the degree of molecular genetic diversity among the 50 cultivated genotypes and five wild species using 17 SSR markers. Seven were monomorphic and 10 markers showed polymorphism. The markers CCB 10, PGM 10, PGM 106, CCM 1538, CCM 1886, CCM 2971, CCM 1263, CCM 0268, CCM 0583, PGM 5 and CCM 1026 showed polymorphism among the genotypes. Microsatellite profiling showed that maximum alleles with amplicon sizes of 150–260 bp. This indicated the effectiveness and higher resolution of such marker systems in detecting molecular diversity. Similar to this, a dendrogram was created using 55 pigeonpea genotypes and highly polymorphic 10 SSR markers which revealed 9 major clusters, ranging from Clusters 1–9, with cluster 6 having the largest number of genotypes (12), and clusters 2, 9 having the smallest number of genotypes (2). This study showed the divergence among pigeonpea genotypes which can be further used in pigeonpea breeding programs. All genotypes involved in this study exhibited a wide range of genetic variability due to different centres of origin, and different genetic constitutions. The genetic relatedness detected in this study may constitute the foundation for future systematic pigeonpea breeding programmes. https://epubs.icar.org.in/index.php/IJAgS/article/view/136823Cajanus cajan, Dendrogram, Genetic diversity, SSR markers |
| spellingShingle | A THANGA HEMAVATHY R KALAIYARASI S KAVITHA N PREMALATHA KAVITHAMANI DURAISAMY NEDUNCHEZHIY VINOTHINI Molecular characterization of wild and cultivated pigeonpea (Cajanus spp.) species by microsatellites The Indian Journal of Agricultural Sciences Cajanus cajan, Dendrogram, Genetic diversity, SSR markers |
| title | Molecular characterization of wild and cultivated pigeonpea (Cajanus spp.) species by microsatellites |
| title_full | Molecular characterization of wild and cultivated pigeonpea (Cajanus spp.) species by microsatellites |
| title_fullStr | Molecular characterization of wild and cultivated pigeonpea (Cajanus spp.) species by microsatellites |
| title_full_unstemmed | Molecular characterization of wild and cultivated pigeonpea (Cajanus spp.) species by microsatellites |
| title_short | Molecular characterization of wild and cultivated pigeonpea (Cajanus spp.) species by microsatellites |
| title_sort | molecular characterization of wild and cultivated pigeonpea cajanus spp species by microsatellites |
| topic | Cajanus cajan, Dendrogram, Genetic diversity, SSR markers |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/136823 |
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