Azelaic acid reduces arsenic-induced toxicity and inflammation in the rat islets of langerhans
Objective(s): Arsenic is classified as a toxic metal that is naturally found in the Earth’s crust, and long-term exposure to it can result in chronic human disorders like cancer and diabetes. Azelaic acid (AZA), a natural dicarboxylic acid, has been reported to have anti-oxidant and anti-inflammator...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2025-06-01
|
| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_25699_b00c27c56874f558d4a98e896bdfdfd5.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849408385313669120 |
|---|---|
| author | Sara Mostafalou Fatemeh Moafi-Madani Maryam Baeeri Mahban Rahimifard Hamed Haghi-Aminjan |
| author_facet | Sara Mostafalou Fatemeh Moafi-Madani Maryam Baeeri Mahban Rahimifard Hamed Haghi-Aminjan |
| author_sort | Sara Mostafalou |
| collection | DOAJ |
| description | Objective(s): Arsenic is classified as a toxic metal that is naturally found in the Earth’s crust, and long-term exposure to it can result in chronic human disorders like cancer and diabetes. Azelaic acid (AZA), a natural dicarboxylic acid, has been reported to have anti-oxidant and anti-inflammatory effects; hence, it may protect against the metabolic toxicity of arsenic. This study aimed to investigate whether AZA could ameliorate sodium arsenite (SA) toxicity toward rat islets of Langerhans. Materials and Methods: Pancreatic Islets of Langerhans isolated from adult male Wistar rats were divided into four groups of 10: control, SA, AZA, and SA plus AZA. Twenty-four hours after incubation, cell viability, cell death pathways, reactive oxygen species (ROS), inflammatory factor gene expression, and insulin secretion were evaluated. Results: SA dose-dependently decreased cell viability, increased apoptosis, ROS generation, expression of inflammatory mediators (NF-κB, IL-1β, and TNF-α), and insulin secretion. AZA was able to ameliorate all these changes significantly. Conclusion: Our results indicate that SA can potentially disrupt cellular homeostasis and function in the islets of Langerhans and can increase the risk of metabolic diseases such as diabetes. On the other hand, AZA protected islets of Langerhans against the toxic effects of SA, seemingly due to its anti-apoptotic, anti-inflammatory, and anti-oxidant properties, indicating that AZA may have the potential to run intracellular mechanisms beneficial for coping with the metabolic toxicity of arsenic. |
| format | Article |
| id | doaj-art-2e44126b6bee489dbe6125acf121e25d |
| institution | Kabale University |
| issn | 2008-3866 2008-3874 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj-art-2e44126b6bee489dbe6125acf121e25d2025-08-20T03:35:48ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742025-06-0128678478910.22038/ijbms.2025.84651.1831125699Azelaic acid reduces arsenic-induced toxicity and inflammation in the rat islets of langerhansSara Mostafalou0Fatemeh Moafi-Madani1Maryam Baeeri2Mahban Rahimifard3Hamed Haghi-Aminjan4Department of Pharmacology and Toxicology, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, IranDepartment of Pharmacology and Toxicology, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, IranToxicology and Diseases Group, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, IranToxicology and Diseases Group, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, IranPharmaceutical Sciences Research Center, Ardabil University of Medical Sciences, Ardabil, IranObjective(s): Arsenic is classified as a toxic metal that is naturally found in the Earth’s crust, and long-term exposure to it can result in chronic human disorders like cancer and diabetes. Azelaic acid (AZA), a natural dicarboxylic acid, has been reported to have anti-oxidant and anti-inflammatory effects; hence, it may protect against the metabolic toxicity of arsenic. This study aimed to investigate whether AZA could ameliorate sodium arsenite (SA) toxicity toward rat islets of Langerhans. Materials and Methods: Pancreatic Islets of Langerhans isolated from adult male Wistar rats were divided into four groups of 10: control, SA, AZA, and SA plus AZA. Twenty-four hours after incubation, cell viability, cell death pathways, reactive oxygen species (ROS), inflammatory factor gene expression, and insulin secretion were evaluated. Results: SA dose-dependently decreased cell viability, increased apoptosis, ROS generation, expression of inflammatory mediators (NF-κB, IL-1β, and TNF-α), and insulin secretion. AZA was able to ameliorate all these changes significantly. Conclusion: Our results indicate that SA can potentially disrupt cellular homeostasis and function in the islets of Langerhans and can increase the risk of metabolic diseases such as diabetes. On the other hand, AZA protected islets of Langerhans against the toxic effects of SA, seemingly due to its anti-apoptotic, anti-inflammatory, and anti-oxidant properties, indicating that AZA may have the potential to run intracellular mechanisms beneficial for coping with the metabolic toxicity of arsenic.https://ijbms.mums.ac.ir/article_25699_b00c27c56874f558d4a98e896bdfdfd5.pdfapoptosisazelaic acidinflammationinsulinoxidative stresssodium arsenite |
| spellingShingle | Sara Mostafalou Fatemeh Moafi-Madani Maryam Baeeri Mahban Rahimifard Hamed Haghi-Aminjan Azelaic acid reduces arsenic-induced toxicity and inflammation in the rat islets of langerhans Iranian Journal of Basic Medical Sciences apoptosis azelaic acid inflammation insulin oxidative stress sodium arsenite |
| title | Azelaic acid reduces arsenic-induced toxicity and inflammation in the rat islets of langerhans |
| title_full | Azelaic acid reduces arsenic-induced toxicity and inflammation in the rat islets of langerhans |
| title_fullStr | Azelaic acid reduces arsenic-induced toxicity and inflammation in the rat islets of langerhans |
| title_full_unstemmed | Azelaic acid reduces arsenic-induced toxicity and inflammation in the rat islets of langerhans |
| title_short | Azelaic acid reduces arsenic-induced toxicity and inflammation in the rat islets of langerhans |
| title_sort | azelaic acid reduces arsenic induced toxicity and inflammation in the rat islets of langerhans |
| topic | apoptosis azelaic acid inflammation insulin oxidative stress sodium arsenite |
| url | https://ijbms.mums.ac.ir/article_25699_b00c27c56874f558d4a98e896bdfdfd5.pdf |
| work_keys_str_mv | AT saramostafalou azelaicacidreducesarsenicinducedtoxicityandinflammationintheratisletsoflangerhans AT fatemehmoafimadani azelaicacidreducesarsenicinducedtoxicityandinflammationintheratisletsoflangerhans AT maryambaeeri azelaicacidreducesarsenicinducedtoxicityandinflammationintheratisletsoflangerhans AT mahbanrahimifard azelaicacidreducesarsenicinducedtoxicityandinflammationintheratisletsoflangerhans AT hamedhaghiaminjan azelaicacidreducesarsenicinducedtoxicityandinflammationintheratisletsoflangerhans |