Phytophthora capsici Epidemic Dispersion on Commercial Pepper Fields in Aguascalientes, Mexico
Chili pepper blight observed on pepper farms from north Aguascalientes was monitored for the presence of Phytophthora capsici during 2008–2010. Initially, ELISA tests were directed to plant samples from greenhouses and rustic nurseries, showing an 86% of positive samples. Later, samples of wilted pl...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/341764 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850106557645193216 |
|---|---|
| author | Adrián Zapata-Vázquez Mario Sánchez-Sánchez Alicia del-Río-Robledo Héctor Silos-Espino Catarino Perales-Segovia Silvia Flores-Benítez Mario Martín González-Chavira Luis Lorenzo Valera-Montero |
| author_facet | Adrián Zapata-Vázquez Mario Sánchez-Sánchez Alicia del-Río-Robledo Héctor Silos-Espino Catarino Perales-Segovia Silvia Flores-Benítez Mario Martín González-Chavira Luis Lorenzo Valera-Montero |
| author_sort | Adrián Zapata-Vázquez |
| collection | DOAJ |
| description | Chili pepper blight observed on pepper farms from north Aguascalientes was monitored for the presence of Phytophthora capsici during 2008–2010. Initially, ELISA tests were directed to plant samples from greenhouses and rustic nurseries, showing an 86% of positive samples. Later, samples of wilted plants from the farms during the first survey were tested with ELISA. The subsequent survey on soil samples included mycelia isolation and PCR amplification of a 560 bp fragment of ITS-specific DNA sequence of P. capsici. Data was analyzed according to four geographical areas defined by coordinates to ease the dispersal assessment. In general, one-third of the samples from surveyed fields contained P. capsici, inferring that this may be the pathogen responsible of the observed wilt. Nevertheless, only five sites from a total of 92 were consistently negative to P. capsici. The presence of this pathogen was detected through ELISA and confirmed through PCR. The other two-thirds of the negative samples may be attributable to Fusarium and Rhizoctonia, both isolated instead of Phytophthora in these areas. Due to these striking results, this information would be of interest for local plant protection committees and farmers to avoid further dispersal of pathogens to new lands. |
| format | Article |
| id | doaj-art-2e3c86b938dc4900ac8c8bb2986103cc |
| institution | OA Journals |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-2e3c86b938dc4900ac8c8bb2986103cc2025-08-20T02:38:48ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/341764341764Phytophthora capsici Epidemic Dispersion on Commercial Pepper Fields in Aguascalientes, MexicoAdrián Zapata-Vázquez0Mario Sánchez-Sánchez1Alicia del-Río-Robledo2Héctor Silos-Espino3Catarino Perales-Segovia4Silvia Flores-Benítez5Mario Martín González-Chavira6Luis Lorenzo Valera-Montero7Instituto Tecnológico El Llano Aguascalientes, Ags., Km 18, Carr. Ags.-S.L.P., 20330, MexicoInstituto Tecnológico El Llano Aguascalientes, Ags., Km 18, Carr. Ags.-S.L.P., 20330, MexicoInstituto Tecnológico El Llano Aguascalientes, Ags., Km 18, Carr. Ags.-S.L.P., 20330, MexicoInstituto Tecnológico El Llano Aguascalientes, Ags., Km 18, Carr. Ags.-S.L.P., 20330, MexicoInstituto Tecnológico El Llano Aguascalientes, Ags., Km 18, Carr. Ags.-S.L.P., 20330, MexicoInstituto Tecnológico El Llano Aguascalientes, Ags., Km 18, Carr. Ags.-S.L.P., 20330, MexicoCIRCE-INIFAP, Km 6.5 Carr. Celaya-San Miguel de Allende, 38110 Celaya, Gto., MexicoInstituto Tecnológico El Llano Aguascalientes, Ags., Km 18, Carr. Ags.-S.L.P., 20330, MexicoChili pepper blight observed on pepper farms from north Aguascalientes was monitored for the presence of Phytophthora capsici during 2008–2010. Initially, ELISA tests were directed to plant samples from greenhouses and rustic nurseries, showing an 86% of positive samples. Later, samples of wilted plants from the farms during the first survey were tested with ELISA. The subsequent survey on soil samples included mycelia isolation and PCR amplification of a 560 bp fragment of ITS-specific DNA sequence of P. capsici. Data was analyzed according to four geographical areas defined by coordinates to ease the dispersal assessment. In general, one-third of the samples from surveyed fields contained P. capsici, inferring that this may be the pathogen responsible of the observed wilt. Nevertheless, only five sites from a total of 92 were consistently negative to P. capsici. The presence of this pathogen was detected through ELISA and confirmed through PCR. The other two-thirds of the negative samples may be attributable to Fusarium and Rhizoctonia, both isolated instead of Phytophthora in these areas. Due to these striking results, this information would be of interest for local plant protection committees and farmers to avoid further dispersal of pathogens to new lands.http://dx.doi.org/10.1100/2012/341764 |
| spellingShingle | Adrián Zapata-Vázquez Mario Sánchez-Sánchez Alicia del-Río-Robledo Héctor Silos-Espino Catarino Perales-Segovia Silvia Flores-Benítez Mario Martín González-Chavira Luis Lorenzo Valera-Montero Phytophthora capsici Epidemic Dispersion on Commercial Pepper Fields in Aguascalientes, Mexico The Scientific World Journal |
| title | Phytophthora capsici Epidemic Dispersion on Commercial Pepper Fields in Aguascalientes, Mexico |
| title_full | Phytophthora capsici Epidemic Dispersion on Commercial Pepper Fields in Aguascalientes, Mexico |
| title_fullStr | Phytophthora capsici Epidemic Dispersion on Commercial Pepper Fields in Aguascalientes, Mexico |
| title_full_unstemmed | Phytophthora capsici Epidemic Dispersion on Commercial Pepper Fields in Aguascalientes, Mexico |
| title_short | Phytophthora capsici Epidemic Dispersion on Commercial Pepper Fields in Aguascalientes, Mexico |
| title_sort | phytophthora capsici epidemic dispersion on commercial pepper fields in aguascalientes mexico |
| url | http://dx.doi.org/10.1100/2012/341764 |
| work_keys_str_mv | AT adrianzapatavazquez phytophthoracapsiciepidemicdispersiononcommercialpepperfieldsinaguascalientesmexico AT mariosanchezsanchez phytophthoracapsiciepidemicdispersiononcommercialpepperfieldsinaguascalientesmexico AT aliciadelriorobledo phytophthoracapsiciepidemicdispersiononcommercialpepperfieldsinaguascalientesmexico AT hectorsilosespino phytophthoracapsiciepidemicdispersiononcommercialpepperfieldsinaguascalientesmexico AT catarinoperalessegovia phytophthoracapsiciepidemicdispersiononcommercialpepperfieldsinaguascalientesmexico AT silviafloresbenitez phytophthoracapsiciepidemicdispersiononcommercialpepperfieldsinaguascalientesmexico AT mariomartingonzalezchavira phytophthoracapsiciepidemicdispersiononcommercialpepperfieldsinaguascalientesmexico AT luislorenzovaleramontero phytophthoracapsiciepidemicdispersiononcommercialpepperfieldsinaguascalientesmexico |