Cytotoxic activity of methanolic fractions of different Marrubium spp. against melanoma cells is independent of antioxidant activity and total phenolic content
The Marrubium genus (horehound) has proved to be an abundant source of biologically active compounds, but there is little knowledge about its potential anticancer activity. Moreover, some Marrubium species have not been the subject of study in this regard. In this study, we performed comparative ana...
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Wiley
2020-01-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.12755 |
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| author | Malgorzata Kozyra Agnieszka Korga Marta Ostrowska Ewelina Humeniuk Grzegorz Adamczuk Renata Gieroba Anna Makuch‐Kocka Jaroslaw Dudka |
| author_facet | Malgorzata Kozyra Agnieszka Korga Marta Ostrowska Ewelina Humeniuk Grzegorz Adamczuk Renata Gieroba Anna Makuch‐Kocka Jaroslaw Dudka |
| author_sort | Malgorzata Kozyra |
| collection | DOAJ |
| description | The Marrubium genus (horehound) has proved to be an abundant source of biologically active compounds, but there is little knowledge about its potential anticancer activity. Moreover, some Marrubium species have not been the subject of study in this regard. In this study, we performed comparative analysis of phenolic acid (PhA) content and total phenolic content in fractions obtained from methanolic extracts of Marrubium vulgare L. (common horehound), Marrubium cylleneum Boiss. & Heldr. and Marrubium friwaldskyanum Boiss herbs. We examined the cytotoxicity of these fractions against a human melanoma cancer cell line (A375) and normal human skin fibroblasts (BJ) using a 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐tetrazolium bromide test, cell cycle analysis and real‐time monitoring of cell viability. We detected caffeic, p‐coumaric, ferulic and gentisic acids among the PhAs. Although the extracts obtained demonstrated low total phenolic content and did not show significant antioxidative properties, the nonhydrolyzed PhA fraction exhibited cytotoxic activity against a human melanoma cancer cell line, without affecting normal fibroblasts. Both acidic and alkaline hydrolysis abolished this activity, indicating that the esterified forms of phenolic compounds caused the observed cytotoxic effects. Further investigation of these compounds may facilitate the development of novel drugs for cancer treatment. |
| format | Article |
| id | doaj-art-2e3728a97c884dbea3c81d5ab39d31f0 |
| institution | OA Journals |
| issn | 2211-5463 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj-art-2e3728a97c884dbea3c81d5ab39d31f02025-08-20T01:54:33ZengWileyFEBS Open Bio2211-54632020-01-01101869510.1002/2211-5463.12755Cytotoxic activity of methanolic fractions of different Marrubium spp. against melanoma cells is independent of antioxidant activity and total phenolic contentMalgorzata Kozyra0Agnieszka Korga1Marta Ostrowska2Ewelina Humeniuk3Grzegorz Adamczuk4Renata Gieroba5Anna Makuch‐Kocka6Jaroslaw Dudka7Department of Pharmacognosy with Medicinal Plant Laboratory Medical University of Lublin PolandIndependent Medical Biology Unit Medical University of Lublin PolandDepartment of Toxicology Medical University of Lublin PolandIndependent Medical Biology Unit Medical University of Lublin PolandIndependent Medical Biology Unit Medical University of Lublin PolandIndependent Medical Biology Unit Medical University of Lublin PolandIndependent Medical Biology Unit Medical University of Lublin PolandDepartment of Toxicology Medical University of Lublin PolandThe Marrubium genus (horehound) has proved to be an abundant source of biologically active compounds, but there is little knowledge about its potential anticancer activity. Moreover, some Marrubium species have not been the subject of study in this regard. In this study, we performed comparative analysis of phenolic acid (PhA) content and total phenolic content in fractions obtained from methanolic extracts of Marrubium vulgare L. (common horehound), Marrubium cylleneum Boiss. & Heldr. and Marrubium friwaldskyanum Boiss herbs. We examined the cytotoxicity of these fractions against a human melanoma cancer cell line (A375) and normal human skin fibroblasts (BJ) using a 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐tetrazolium bromide test, cell cycle analysis and real‐time monitoring of cell viability. We detected caffeic, p‐coumaric, ferulic and gentisic acids among the PhAs. Although the extracts obtained demonstrated low total phenolic content and did not show significant antioxidative properties, the nonhydrolyzed PhA fraction exhibited cytotoxic activity against a human melanoma cancer cell line, without affecting normal fibroblasts. Both acidic and alkaline hydrolysis abolished this activity, indicating that the esterified forms of phenolic compounds caused the observed cytotoxic effects. Further investigation of these compounds may facilitate the development of novel drugs for cancer treatment.https://doi.org/10.1002/2211-5463.12755antioxidant activityfree phenolic acidin vitro cytotoxicityMarrubiummelanoma |
| spellingShingle | Malgorzata Kozyra Agnieszka Korga Marta Ostrowska Ewelina Humeniuk Grzegorz Adamczuk Renata Gieroba Anna Makuch‐Kocka Jaroslaw Dudka Cytotoxic activity of methanolic fractions of different Marrubium spp. against melanoma cells is independent of antioxidant activity and total phenolic content FEBS Open Bio antioxidant activity free phenolic acid in vitro cytotoxicity Marrubium melanoma |
| title | Cytotoxic activity of methanolic fractions of different Marrubium spp. against melanoma cells is independent of antioxidant activity and total phenolic content |
| title_full | Cytotoxic activity of methanolic fractions of different Marrubium spp. against melanoma cells is independent of antioxidant activity and total phenolic content |
| title_fullStr | Cytotoxic activity of methanolic fractions of different Marrubium spp. against melanoma cells is independent of antioxidant activity and total phenolic content |
| title_full_unstemmed | Cytotoxic activity of methanolic fractions of different Marrubium spp. against melanoma cells is independent of antioxidant activity and total phenolic content |
| title_short | Cytotoxic activity of methanolic fractions of different Marrubium spp. against melanoma cells is independent of antioxidant activity and total phenolic content |
| title_sort | cytotoxic activity of methanolic fractions of different marrubium spp against melanoma cells is independent of antioxidant activity and total phenolic content |
| topic | antioxidant activity free phenolic acid in vitro cytotoxicity Marrubium melanoma |
| url | https://doi.org/10.1002/2211-5463.12755 |
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