Synergistic degradation of wheat by Daqu complex enzyme system
To study the degradation of the Daqu complex enzyme system on the natural substrate wheat, two α-amylases (NFAmy13B and NFAmy13A), endo-β-glucanase NFEg16 from strong-flavor (Nongxiangxing) Baiju Daqu, as well as xylanase TbXyn10A, and two xylosidases (TbXyl39A and TbXyl52A) from Thermoanaerobacteri...
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Editorial Department of China Brewing
2024-03-01
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| Series: | Zhongguo niangzao |
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| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-3-56.pdf |
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| author | JIANG Yijia, YI Zhuolin, ZHAO Hai, REN Zhiqiang |
| author_facet | JIANG Yijia, YI Zhuolin, ZHAO Hai, REN Zhiqiang |
| author_sort | JIANG Yijia, YI Zhuolin, ZHAO Hai, REN Zhiqiang |
| collection | DOAJ |
| description | To study the degradation of the Daqu complex enzyme system on the natural substrate wheat, two α-amylases (NFAmy13B and NFAmy13A), endo-β-glucanase NFEg16 from strong-flavor (Nongxiangxing) Baiju Daqu, as well as xylanase TbXyn10A, and two xylosidases (TbXyl39A and TbXyl52A) from Thermoanaerobacterium bryantii mel9<sup>T</sup>, were heterologouly expressed and purified using affinity chromatography methods. Using wheat crushing material as substrate, the effects of different enzymes and ratios on wheat degradation were studied, and the degradation effect was evaluated by measuring the content of reducing sugar by p-hydroxybenzoic acid (pHBAH) method. The results shown that Daqu α-amylases NFAmy13B was a typical liquefying α-amylase, which could efficient degrade long-chain starch, and produced 5.12 mmol/L reducing glucose by degrading wheat powder for 4 h. NFAmy13A was a typical maltogenic α-amylase, which could efficient degrade short-chain starch into maltose, and together with NFEg16A, significantly improved the degradation of NFAmy13B on wheat powder, with their compound hydrolysis efficiency being 2.6 times that of NFAmy13B alone. Combining xylanase and xylosidase with the former three enzymes could further improve wheat degradation, achieving 2.9 times that of NFAmy13B alone. This study confirmed that Daqu complex enzyme system had synergistic degradation effect on wheat powder. |
| format | Article |
| id | doaj-art-2e2d3fd239534e4f90acfdf09b343e1d |
| institution | DOAJ |
| issn | 0254-5071 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Editorial Department of China Brewing |
| record_format | Article |
| series | Zhongguo niangzao |
| spelling | doaj-art-2e2d3fd239534e4f90acfdf09b343e1d2025-08-20T03:04:46ZengEditorial Department of China BrewingZhongguo niangzao0254-50712024-03-01433566210.11882/j.issn.0254-5071.2024.03.009Synergistic degradation of wheat by Daqu complex enzyme systemJIANG Yijia, YI Zhuolin, ZHAO Hai, REN Zhiqiang01. Liquor Brewing Biotechnology and Application Key Laboratory of Sichuan Province, School of Biological Engineering, Sichuan University of Science & Engineering, Yibin 644000, China;;2. Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, CAS Key Laboratory of Environmental and Applied Microbiology, Chinese Academy of Sciences, Chengdu 610041, ChinaTo study the degradation of the Daqu complex enzyme system on the natural substrate wheat, two α-amylases (NFAmy13B and NFAmy13A), endo-β-glucanase NFEg16 from strong-flavor (Nongxiangxing) Baiju Daqu, as well as xylanase TbXyn10A, and two xylosidases (TbXyl39A and TbXyl52A) from Thermoanaerobacterium bryantii mel9<sup>T</sup>, were heterologouly expressed and purified using affinity chromatography methods. Using wheat crushing material as substrate, the effects of different enzymes and ratios on wheat degradation were studied, and the degradation effect was evaluated by measuring the content of reducing sugar by p-hydroxybenzoic acid (pHBAH) method. The results shown that Daqu α-amylases NFAmy13B was a typical liquefying α-amylase, which could efficient degrade long-chain starch, and produced 5.12 mmol/L reducing glucose by degrading wheat powder for 4 h. NFAmy13A was a typical maltogenic α-amylase, which could efficient degrade short-chain starch into maltose, and together with NFEg16A, significantly improved the degradation of NFAmy13B on wheat powder, with their compound hydrolysis efficiency being 2.6 times that of NFAmy13B alone. Combining xylanase and xylosidase with the former three enzymes could further improve wheat degradation, achieving 2.9 times that of NFAmy13B alone. This study confirmed that Daqu complex enzyme system had synergistic degradation effect on wheat powder.https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-3-56.pdfdaqu complex enzyme system|wheat|synergistic degradation |
| spellingShingle | JIANG Yijia, YI Zhuolin, ZHAO Hai, REN Zhiqiang Synergistic degradation of wheat by Daqu complex enzyme system Zhongguo niangzao daqu complex enzyme system|wheat|synergistic degradation |
| title | Synergistic degradation of wheat by Daqu complex enzyme system |
| title_full | Synergistic degradation of wheat by Daqu complex enzyme system |
| title_fullStr | Synergistic degradation of wheat by Daqu complex enzyme system |
| title_full_unstemmed | Synergistic degradation of wheat by Daqu complex enzyme system |
| title_short | Synergistic degradation of wheat by Daqu complex enzyme system |
| title_sort | synergistic degradation of wheat by daqu complex enzyme system |
| topic | daqu complex enzyme system|wheat|synergistic degradation |
| url | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-3-56.pdf |
| work_keys_str_mv | AT jiangyijiayizhuolinzhaohairenzhiqiang synergisticdegradationofwheatbydaqucomplexenzymesystem |