Role of DPP-4 and NPY Family Peptides in Gastrointestinal Symptoms Associated with Obesity and Type 2 Diabetes Mellitus
<i>Background and Objectives</i>: Neuropeptide Y (NPY) family peptides and dipeptidyl peptidase-4 (DPP-4) are involved in gastrointestinal regulation and may contribute to obesity and type 2 diabetes mellitus (T2DM) pathophysiology. This study investigates their expression in jejunal mus...
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2025-03-01
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| author | Mantas Malinauskas Deimante Paskeviciene Rūta Steponaitienė Rita Gudaityte Limas Kupčinskas Anna Casselbrant Almantas Maleckas |
| author_facet | Mantas Malinauskas Deimante Paskeviciene Rūta Steponaitienė Rita Gudaityte Limas Kupčinskas Anna Casselbrant Almantas Maleckas |
| author_sort | Mantas Malinauskas |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: Neuropeptide Y (NPY) family peptides and dipeptidyl peptidase-4 (DPP-4) are involved in gastrointestinal regulation and may contribute to obesity and type 2 diabetes mellitus (T2DM) pathophysiology. This study investigates their expression in jejunal muscular tissue and associations with gastrointestinal symptoms in patients with obesity, with (OB+/DM+) and without T2DM (OB+/DM−). <i>Materials and Methods</i>: This cross-sectional study includes forty-four patients undergoing laparoscopic Roux-en-Y gastric bypass divided based on T2DM status. Gastrointestinal symptoms were assessed using the Gastrointestinal Symptom Rating Scale (GSRS) questionnaire, and jejunal tissue samples were analyzed for DPP-4, NPY, peptide YY (PYY), and pancreatic polypeptide (PP) mRNA and protein levels. <i>Results</i>: DPP-4, NPY, PYY, and PP gene expression in jejunal muscular tissue was similar between groups. In the OB+/DM+ group, PP protein was higher, while DPP-4 and PYY were lower compared to the OB+/DM− group. Significant positive correlations between DPP-4 and NPY, PYY, and PP were found in the OB+/DM− group, while only DPP-4 and PYY correlated in the OB+/DM+ group. Gastrointestinal symptoms in the OB+/DM− group showed positive correlations with PP (abdominal pain), DPP-4 (indigestion), and NPY (constipation). <i>Conclusions</i>: The study demonstrates significant differences in DPP-4, PYY, and PP protein expression between patients with obesity, with or without T2DM. Peptide correlations with gastrointestinal symptoms in non-diabetic patients suggest distinct regulatory mechanisms, warranting further research. |
| format | Article |
| id | doaj-art-2e29cd7e21424cef96f5ab83e82042c8 |
| institution | Kabale University |
| issn | 1010-660X 1648-9144 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj-art-2e29cd7e21424cef96f5ab83e82042c82025-08-20T03:43:31ZengMDPI AGMedicina1010-660X1648-91442025-03-0161350410.3390/medicina61030504Role of DPP-4 and NPY Family Peptides in Gastrointestinal Symptoms Associated with Obesity and Type 2 Diabetes MellitusMantas Malinauskas0Deimante Paskeviciene1Rūta Steponaitienė2Rita Gudaityte3Limas Kupčinskas4Anna Casselbrant5Almantas Maleckas6Institute of Physiology and Pharmacology, Lithuanian University of Health Sciences, 44307 Kaunas, LithuaniaInstitute of Endocrinology, Medical Academy, Lithuanian University of Health Sciences, 50161 Kaunas, LithuaniaInstitute for Digestive Research, Lithuanian University of Health Sciences, 50161 Kaunas, LithuaniaDepartment of Surgery, Lithuanian University of Health Sciences, 50161 Kaunas, LithuaniaInstitute for Digestive Research, Lithuanian University of Health Sciences, 50161 Kaunas, LithuaniaDepartment of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, 413 45 Goteborg, SwedenDepartment of Surgery, Lithuanian University of Health Sciences, 50161 Kaunas, Lithuania<i>Background and Objectives</i>: Neuropeptide Y (NPY) family peptides and dipeptidyl peptidase-4 (DPP-4) are involved in gastrointestinal regulation and may contribute to obesity and type 2 diabetes mellitus (T2DM) pathophysiology. This study investigates their expression in jejunal muscular tissue and associations with gastrointestinal symptoms in patients with obesity, with (OB+/DM+) and without T2DM (OB+/DM−). <i>Materials and Methods</i>: This cross-sectional study includes forty-four patients undergoing laparoscopic Roux-en-Y gastric bypass divided based on T2DM status. Gastrointestinal symptoms were assessed using the Gastrointestinal Symptom Rating Scale (GSRS) questionnaire, and jejunal tissue samples were analyzed for DPP-4, NPY, peptide YY (PYY), and pancreatic polypeptide (PP) mRNA and protein levels. <i>Results</i>: DPP-4, NPY, PYY, and PP gene expression in jejunal muscular tissue was similar between groups. In the OB+/DM+ group, PP protein was higher, while DPP-4 and PYY were lower compared to the OB+/DM− group. Significant positive correlations between DPP-4 and NPY, PYY, and PP were found in the OB+/DM− group, while only DPP-4 and PYY correlated in the OB+/DM+ group. Gastrointestinal symptoms in the OB+/DM− group showed positive correlations with PP (abdominal pain), DPP-4 (indigestion), and NPY (constipation). <i>Conclusions</i>: The study demonstrates significant differences in DPP-4, PYY, and PP protein expression between patients with obesity, with or without T2DM. Peptide correlations with gastrointestinal symptoms in non-diabetic patients suggest distinct regulatory mechanisms, warranting further research.https://www.mdpi.com/1648-9144/61/3/504dipeptidyl peptidase-4obesitytype 2 diabetesneuropeptide Ypeptide YYpancreatic polypeptide |
| spellingShingle | Mantas Malinauskas Deimante Paskeviciene Rūta Steponaitienė Rita Gudaityte Limas Kupčinskas Anna Casselbrant Almantas Maleckas Role of DPP-4 and NPY Family Peptides in Gastrointestinal Symptoms Associated with Obesity and Type 2 Diabetes Mellitus Medicina dipeptidyl peptidase-4 obesity type 2 diabetes neuropeptide Y peptide YY pancreatic polypeptide |
| title | Role of DPP-4 and NPY Family Peptides in Gastrointestinal Symptoms Associated with Obesity and Type 2 Diabetes Mellitus |
| title_full | Role of DPP-4 and NPY Family Peptides in Gastrointestinal Symptoms Associated with Obesity and Type 2 Diabetes Mellitus |
| title_fullStr | Role of DPP-4 and NPY Family Peptides in Gastrointestinal Symptoms Associated with Obesity and Type 2 Diabetes Mellitus |
| title_full_unstemmed | Role of DPP-4 and NPY Family Peptides in Gastrointestinal Symptoms Associated with Obesity and Type 2 Diabetes Mellitus |
| title_short | Role of DPP-4 and NPY Family Peptides in Gastrointestinal Symptoms Associated with Obesity and Type 2 Diabetes Mellitus |
| title_sort | role of dpp 4 and npy family peptides in gastrointestinal symptoms associated with obesity and type 2 diabetes mellitus |
| topic | dipeptidyl peptidase-4 obesity type 2 diabetes neuropeptide Y peptide YY pancreatic polypeptide |
| url | https://www.mdpi.com/1648-9144/61/3/504 |
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