Food-derived extracellular vesicles for treatment of osteoporosis: a systematic review and meta-analysis of preclinical animal studies

Abstract Objective Food-derived extracellular vesicles (EVs) are considered a novel therapeutic approach for osteoporosis. However, their efficacy in treating osteoporosis remains unclear. This systematic review and meta-analysis aim to preliminarily evaluate and explore the effects of food-derived...

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Main Authors: Hongchen Shi, Ying Zhang, Qingsong Zhu, Yuntong Lian, Weiqiang Geng
Format: Article
Language:English
Published: BMC 2025-08-01
Series:European Journal of Medical Research
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Online Access:https://doi.org/10.1186/s40001-025-02944-2
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author Hongchen Shi
Ying Zhang
Qingsong Zhu
Yuntong Lian
Weiqiang Geng
author_facet Hongchen Shi
Ying Zhang
Qingsong Zhu
Yuntong Lian
Weiqiang Geng
author_sort Hongchen Shi
collection DOAJ
description Abstract Objective Food-derived extracellular vesicles (EVs) are considered a novel therapeutic approach for osteoporosis. However, their efficacy in treating osteoporosis remains unclear. This systematic review and meta-analysis aim to preliminarily evaluate and explore the effects of food-derived EVs in animal models of osteoporosis. Methods PubMed, Embase, and Web of Science databases were searched for preclinical studies on food-derived EVs in the treatment of osteoporosis, up to November 25, 2024. A meta-analysis was conducted to evaluate the effects of food-derived EVs on bone mineral density (BMD), bone volume/total volume (BV/TV), trabecular thickness (Tb.Th), trabecular separation/marrow thickness (Tb.Sp), trabecular number (Tb.N), and serum bone turnover markers in osteoporosis animal models. Results Six studies met the inclusion criteria for the meta-analysis. The results showed that food-derived EVs contributed to increases in BMD and bone mass markers (BV/TV, Tb.Th, and Tb.N), while promoting the expression of bone formation markers (OPG and PINP) and reducing the expression of bone resorption markers (TRACP 5b and β-CTX). Sensitivity analysis demonstrated the stability of the pooled effect sizes. Conclusions The present meta-analysis suggested that food-derived EVs could improve BMD and bone mass in animal models of osteoporosis. This positive effect may be attributed to the promotion of bone formation mediated by the upregulation of osteogenic markers and the inhibition of bone resorption mediated by the downregulation of osteoclastic markers. Moreover, it is necessary to conduct studies with larger sample sizes and using other animal models to provide important insights for clinical trials.
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spelling doaj-art-2e277a4a9b91481ea68b376489465c762025-08-24T11:15:19ZengBMCEuropean Journal of Medical Research2047-783X2025-08-0130111410.1186/s40001-025-02944-2Food-derived extracellular vesicles for treatment of osteoporosis: a systematic review and meta-analysis of preclinical animal studiesHongchen Shi0Ying Zhang1Qingsong Zhu2Yuntong Lian3Weiqiang Geng4Department of Orthopedics, Bethune international peace hospitalDepartment of Orthopedics, The Affiliated Hospital Southwest Medical UniversityDepartment of Orthopedics, Bethune international peace hospitalDepartment of Orthopedics, Bethune international peace hospitalDepartment of Orthopedics, Bethune international peace hospitalAbstract Objective Food-derived extracellular vesicles (EVs) are considered a novel therapeutic approach for osteoporosis. However, their efficacy in treating osteoporosis remains unclear. This systematic review and meta-analysis aim to preliminarily evaluate and explore the effects of food-derived EVs in animal models of osteoporosis. Methods PubMed, Embase, and Web of Science databases were searched for preclinical studies on food-derived EVs in the treatment of osteoporosis, up to November 25, 2024. A meta-analysis was conducted to evaluate the effects of food-derived EVs on bone mineral density (BMD), bone volume/total volume (BV/TV), trabecular thickness (Tb.Th), trabecular separation/marrow thickness (Tb.Sp), trabecular number (Tb.N), and serum bone turnover markers in osteoporosis animal models. Results Six studies met the inclusion criteria for the meta-analysis. The results showed that food-derived EVs contributed to increases in BMD and bone mass markers (BV/TV, Tb.Th, and Tb.N), while promoting the expression of bone formation markers (OPG and PINP) and reducing the expression of bone resorption markers (TRACP 5b and β-CTX). Sensitivity analysis demonstrated the stability of the pooled effect sizes. Conclusions The present meta-analysis suggested that food-derived EVs could improve BMD and bone mass in animal models of osteoporosis. This positive effect may be attributed to the promotion of bone formation mediated by the upregulation of osteogenic markers and the inhibition of bone resorption mediated by the downregulation of osteoclastic markers. Moreover, it is necessary to conduct studies with larger sample sizes and using other animal models to provide important insights for clinical trials.https://doi.org/10.1186/s40001-025-02944-2Extracellular vesicleOsteoporosisFoodPreclinical studyMeta-analysis
spellingShingle Hongchen Shi
Ying Zhang
Qingsong Zhu
Yuntong Lian
Weiqiang Geng
Food-derived extracellular vesicles for treatment of osteoporosis: a systematic review and meta-analysis of preclinical animal studies
European Journal of Medical Research
Extracellular vesicle
Osteoporosis
Food
Preclinical study
Meta-analysis
title Food-derived extracellular vesicles for treatment of osteoporosis: a systematic review and meta-analysis of preclinical animal studies
title_full Food-derived extracellular vesicles for treatment of osteoporosis: a systematic review and meta-analysis of preclinical animal studies
title_fullStr Food-derived extracellular vesicles for treatment of osteoporosis: a systematic review and meta-analysis of preclinical animal studies
title_full_unstemmed Food-derived extracellular vesicles for treatment of osteoporosis: a systematic review and meta-analysis of preclinical animal studies
title_short Food-derived extracellular vesicles for treatment of osteoporosis: a systematic review and meta-analysis of preclinical animal studies
title_sort food derived extracellular vesicles for treatment of osteoporosis a systematic review and meta analysis of preclinical animal studies
topic Extracellular vesicle
Osteoporosis
Food
Preclinical study
Meta-analysis
url https://doi.org/10.1186/s40001-025-02944-2
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AT qingsongzhu foodderivedextracellularvesiclesfortreatmentofosteoporosisasystematicreviewandmetaanalysisofpreclinicalanimalstudies
AT yuntonglian foodderivedextracellularvesiclesfortreatmentofosteoporosisasystematicreviewandmetaanalysisofpreclinicalanimalstudies
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