Matings Between Individuals with Similar Major Histocompatibility Complex (MHC) Improve Offspring Survival in the Rainbow Trout (<i>Oncorhynchus mykiss</i>)

The major histocompatibility complex (MHC) consists of genes involved in immune response and molecular discrimination between self and non-self. MHC genes are the most polymorphic in vertebrates. The origin and maintenance of polymorphism in MHC genes in populations is still unresolved. Mechanisms s...

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Main Authors: Cinzia Bergamino, Mattia Tomasoni, Silvia Colussi, Vittoria Montemurro, Ilhan Altinok, Marino Prearo, Charalampos Kotzamanidis, Pier Luigi Acutis, Ana Isabel Vela, Simona Sciuto, Giuseppe Esposito, Donatella Volpatti, Elena Bozzetta, Katia Parati, Lucia Aidos, Lucio Fariano, Benedetto Sicuro
Format: Article
Language:English
Published: MDPI AG 2024-10-01
Series:Fishes
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Online Access:https://www.mdpi.com/2410-3888/9/11/436
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author Cinzia Bergamino
Mattia Tomasoni
Silvia Colussi
Vittoria Montemurro
Ilhan Altinok
Marino Prearo
Charalampos Kotzamanidis
Pier Luigi Acutis
Ana Isabel Vela
Simona Sciuto
Giuseppe Esposito
Donatella Volpatti
Elena Bozzetta
Katia Parati
Lucia Aidos
Lucio Fariano
Benedetto Sicuro
author_facet Cinzia Bergamino
Mattia Tomasoni
Silvia Colussi
Vittoria Montemurro
Ilhan Altinok
Marino Prearo
Charalampos Kotzamanidis
Pier Luigi Acutis
Ana Isabel Vela
Simona Sciuto
Giuseppe Esposito
Donatella Volpatti
Elena Bozzetta
Katia Parati
Lucia Aidos
Lucio Fariano
Benedetto Sicuro
author_sort Cinzia Bergamino
collection DOAJ
description The major histocompatibility complex (MHC) consists of genes involved in immune response and molecular discrimination between self and non-self. MHC genes are the most polymorphic in vertebrates. The origin and maintenance of polymorphism in MHC genes in populations is still unresolved. Mechanisms such as sexual selection and heterozygote advantage have been suggested as explanations for this high variability. In this study, a farmed population of rainbow trout (<i>Oncorhynchus mykiss</i>) characterized by the presence of specific MHC class IIB gene haplotypes at a frequency higher (30%) than that expected from random matings was investigated. Therefore, it was hypothesized that disassortative matings occur with an adaptive advantage for females, resulting in improved reproductive performance when mated with individuals with similar MHC haplotypes. Genetic analyses of the breeders were performed to define the MHC haplotypes and to perform specific matings. The effect of mating was evaluated by analyzing the survival rate of the offspring at various stages of incubation until swim-up. The reproductive performance of the offspring derived from specimens with similar haplotypes showed a better survival trend during the first life stages and reduced malformations. The results obtained are in contrast with the heterozygous advantage theory, therefore it was hypothesized, as for other salmonid species, the presence of a positive selection towards locally adapted MHC genes that promotes reproduction between genetically similar individuals.
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spelling doaj-art-2e227681a6be424bb41423ff2af735672025-08-20T01:53:45ZengMDPI AGFishes2410-38882024-10-0191143610.3390/fishes9110436Matings Between Individuals with Similar Major Histocompatibility Complex (MHC) Improve Offspring Survival in the Rainbow Trout (<i>Oncorhynchus mykiss</i>)Cinzia Bergamino0Mattia Tomasoni1Silvia Colussi2Vittoria Montemurro3Ilhan Altinok4Marino Prearo5Charalampos Kotzamanidis6Pier Luigi Acutis7Ana Isabel Vela8Simona Sciuto9Giuseppe Esposito10Donatella Volpatti11Elena Bozzetta12Katia Parati13Lucia Aidos14Lucio Fariano15Benedetto Sicuro16Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, Turin, ItalyAzienda Agricola Canali Cavour, 12044 Centallo, Cuneo, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, Turin, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, Turin, ItalyFaculty of Marine Sciences, Karadeniz Technical University, 61530 Sürmene, Trabzon, TurkeyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, Turin, ItalyVeterinary Research Institute, ELGO-DIMITRA, 57001 Thessaloniki, GreeceIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, Turin, ItalyCenter for Veterinary Health Surveillance, Universidad Complutense de Madrid, 28040 Madrid, SpainIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, Turin, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, Turin, ItalyDepartment of Agricultural, Food, Environmental and Animal Sciences, University of Udine, 33100 Udine, Udine, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, Turin, ItalyIstituto Sperimentale Italiano L. Spallanzani, 26027 Rivolta d’Adda, Cremona, ItalyDepartment of Veterinary Sciences and Animal Science, University of Milan, 26900 Lodi, Lodi, ItalyAzienda Agricola Canali Cavour, 12044 Centallo, Cuneo, ItalyDepartment of Veterinary Sciences, University of Turin, 10095 Grugliasco, Torino, ItalyThe major histocompatibility complex (MHC) consists of genes involved in immune response and molecular discrimination between self and non-self. MHC genes are the most polymorphic in vertebrates. The origin and maintenance of polymorphism in MHC genes in populations is still unresolved. Mechanisms such as sexual selection and heterozygote advantage have been suggested as explanations for this high variability. In this study, a farmed population of rainbow trout (<i>Oncorhynchus mykiss</i>) characterized by the presence of specific MHC class IIB gene haplotypes at a frequency higher (30%) than that expected from random matings was investigated. Therefore, it was hypothesized that disassortative matings occur with an adaptive advantage for females, resulting in improved reproductive performance when mated with individuals with similar MHC haplotypes. Genetic analyses of the breeders were performed to define the MHC haplotypes and to perform specific matings. The effect of mating was evaluated by analyzing the survival rate of the offspring at various stages of incubation until swim-up. The reproductive performance of the offspring derived from specimens with similar haplotypes showed a better survival trend during the first life stages and reduced malformations. The results obtained are in contrast with the heterozygous advantage theory, therefore it was hypothesized, as for other salmonid species, the presence of a positive selection towards locally adapted MHC genes that promotes reproduction between genetically similar individuals.https://www.mdpi.com/2410-3888/9/11/436similar haplotypeoffspring survivalreproductive performancelarvae deformity
spellingShingle Cinzia Bergamino
Mattia Tomasoni
Silvia Colussi
Vittoria Montemurro
Ilhan Altinok
Marino Prearo
Charalampos Kotzamanidis
Pier Luigi Acutis
Ana Isabel Vela
Simona Sciuto
Giuseppe Esposito
Donatella Volpatti
Elena Bozzetta
Katia Parati
Lucia Aidos
Lucio Fariano
Benedetto Sicuro
Matings Between Individuals with Similar Major Histocompatibility Complex (MHC) Improve Offspring Survival in the Rainbow Trout (<i>Oncorhynchus mykiss</i>)
Fishes
similar haplotype
offspring survival
reproductive performance
larvae deformity
title Matings Between Individuals with Similar Major Histocompatibility Complex (MHC) Improve Offspring Survival in the Rainbow Trout (<i>Oncorhynchus mykiss</i>)
title_full Matings Between Individuals with Similar Major Histocompatibility Complex (MHC) Improve Offspring Survival in the Rainbow Trout (<i>Oncorhynchus mykiss</i>)
title_fullStr Matings Between Individuals with Similar Major Histocompatibility Complex (MHC) Improve Offspring Survival in the Rainbow Trout (<i>Oncorhynchus mykiss</i>)
title_full_unstemmed Matings Between Individuals with Similar Major Histocompatibility Complex (MHC) Improve Offspring Survival in the Rainbow Trout (<i>Oncorhynchus mykiss</i>)
title_short Matings Between Individuals with Similar Major Histocompatibility Complex (MHC) Improve Offspring Survival in the Rainbow Trout (<i>Oncorhynchus mykiss</i>)
title_sort matings between individuals with similar major histocompatibility complex mhc improve offspring survival in the rainbow trout i oncorhynchus mykiss i
topic similar haplotype
offspring survival
reproductive performance
larvae deformity
url https://www.mdpi.com/2410-3888/9/11/436
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