Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine.
Streptococcus pneumoniae causes invasive infections, primarily at the extremes of life. A seven-valent conjugate vaccine (PCV7) is used to protect against invasive pneumococcal disease in children. Within three years of PCV7 introduction, we observed a fourfold increase in serotype 6C carriage, pred...
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Public Library of Science (PLoS)
2013-01-01
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| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0064731&type=printable |
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| author | Nicholas J Loman Rebecca A Gladstone Chrystala Constantinidou Anna S Tocheva Johanna M C Jefferies Saul N Faust Leigh O'Connor Jacqueline Chan Mark J Pallen Stuart C Clarke |
| author_facet | Nicholas J Loman Rebecca A Gladstone Chrystala Constantinidou Anna S Tocheva Johanna M C Jefferies Saul N Faust Leigh O'Connor Jacqueline Chan Mark J Pallen Stuart C Clarke |
| author_sort | Nicholas J Loman |
| collection | DOAJ |
| description | Streptococcus pneumoniae causes invasive infections, primarily at the extremes of life. A seven-valent conjugate vaccine (PCV7) is used to protect against invasive pneumococcal disease in children. Within three years of PCV7 introduction, we observed a fourfold increase in serotype 6C carriage, predominantly due to a single clone. We determined the whole-genome sequences of nineteen S. pneumoniae serotype 6C isolates, from both carriage (n = 15) and disease (n = 4) states, to investigate the emergence of serotype 6C in our population, focusing on a single multi-locus sequence type (MLST) clonal complex 395 (CC395). A phylogenetic network was constructed to identify different lineages, followed by analysis of variability in gene sets and sequences. Serotype 6C isolates from this single geographical site fell into four broad phylogenetically distinct lineages. Variation was seen in the 6C capsular locus and in sequences of genes encoding surface proteins. The largest clonal complex was characterised by the presence of lantibiotic synthesis locus. In our population, the 6C capsular locus has been introduced into multiple lineages by independent capsular switching events. However, rapid clonal expansion has occurred within a single MLST clonal complex. Worryingly, plasticity exists within current and potential vaccine-associated loci, a consideration for future vaccine use, target selection and design. |
| format | Article |
| id | doaj-art-2e1b479eb8eb4142ac87d2e52d4484e4 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-2e1b479eb8eb4142ac87d2e52d4484e42025-08-20T02:05:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0185e6473110.1371/journal.pone.0064731Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine.Nicholas J LomanRebecca A GladstoneChrystala ConstantinidouAnna S TochevaJohanna M C JefferiesSaul N FaustLeigh O'ConnorJacqueline ChanMark J PallenStuart C ClarkeStreptococcus pneumoniae causes invasive infections, primarily at the extremes of life. A seven-valent conjugate vaccine (PCV7) is used to protect against invasive pneumococcal disease in children. Within three years of PCV7 introduction, we observed a fourfold increase in serotype 6C carriage, predominantly due to a single clone. We determined the whole-genome sequences of nineteen S. pneumoniae serotype 6C isolates, from both carriage (n = 15) and disease (n = 4) states, to investigate the emergence of serotype 6C in our population, focusing on a single multi-locus sequence type (MLST) clonal complex 395 (CC395). A phylogenetic network was constructed to identify different lineages, followed by analysis of variability in gene sets and sequences. Serotype 6C isolates from this single geographical site fell into four broad phylogenetically distinct lineages. Variation was seen in the 6C capsular locus and in sequences of genes encoding surface proteins. The largest clonal complex was characterised by the presence of lantibiotic synthesis locus. In our population, the 6C capsular locus has been introduced into multiple lineages by independent capsular switching events. However, rapid clonal expansion has occurred within a single MLST clonal complex. Worryingly, plasticity exists within current and potential vaccine-associated loci, a consideration for future vaccine use, target selection and design.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0064731&type=printable |
| spellingShingle | Nicholas J Loman Rebecca A Gladstone Chrystala Constantinidou Anna S Tocheva Johanna M C Jefferies Saul N Faust Leigh O'Connor Jacqueline Chan Mark J Pallen Stuart C Clarke Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine. PLoS ONE |
| title | Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine. |
| title_full | Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine. |
| title_fullStr | Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine. |
| title_full_unstemmed | Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine. |
| title_short | Clonal expansion within pneumococcal serotype 6C after use of seven-valent vaccine. |
| title_sort | clonal expansion within pneumococcal serotype 6c after use of seven valent vaccine |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0064731&type=printable |
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