Optimum heat reflux extraction of key bioactive compounds from lemon scented tea tree (Leptospermum petersonii) leaves and their cytotoxic effects
The lemon-scented tea tree (Leptospermum petersonii) is rich in bioactive compounds with therapeutic potential; however, optimal extraction conditions for these compounds remain undefined. This study systematically optimised reflux extraction for 16 key bioactives from L. petersonii leaves. Solvent...
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Taylor & Francis Group
2025-08-01
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| Series: | Cogent Food & Agriculture |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/23311932.2025.2544962 |
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| author | Phuong Lan Hoang Alessandro Castorina Yuen Yee Cheng Quan Van Vuong |
| author_facet | Phuong Lan Hoang Alessandro Castorina Yuen Yee Cheng Quan Van Vuong |
| author_sort | Phuong Lan Hoang |
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| description | The lemon-scented tea tree (Leptospermum petersonii) is rich in bioactive compounds with therapeutic potential; however, optimal extraction conditions for these compounds remain undefined. This study systematically optimised reflux extraction for 16 key bioactives from L. petersonii leaves. Solvent type and extraction conditions significantly influenced extraction yields, with 60% ethanol identified as the most effective solvent. Response surface methodology robustly modelled the effects of temperature, reflux time, and sample-to-solvent ratio (lack of fit = 0.1773; p = 0.0069; R² = 0.96). The predicted optimal conditions (95 °C, 160 min, 6:100 g/mL) yielded 39 mg/g of total bioactives. For improved cost-efficiency, extraction at 95 °C for 180 min with a 10:100 g/mL ratio was recommended, reducing solvent use by 40% with only an 8% yield loss, producing 35.8 mg/g bioactives. This extract exhibited strong cytotoxicity against prostate cancer (LNCaP), melanoma (A375, COLO 679), and mesothelioma (AC29) cell lines, with IC₅。 values < 2.50 μg/mL, while showing reduced toxicity toward non-cancerous BV2 cells. These findings establish efficient, scalable extraction conditions and highlight the anticancer potential of L. petersonii, warranting further isolation and mechanistic studies of its active constituents. |
| format | Article |
| id | doaj-art-2e18c7c77430409eaa84d5a7d06deb50 |
| institution | Kabale University |
| issn | 2331-1932 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cogent Food & Agriculture |
| spelling | doaj-art-2e18c7c77430409eaa84d5a7d06deb502025-08-20T04:00:53ZengTaylor & Francis GroupCogent Food & Agriculture2331-19322025-08-0111110.1080/23311932.2025.2544962Optimum heat reflux extraction of key bioactive compounds from lemon scented tea tree (Leptospermum petersonii) leaves and their cytotoxic effectsPhuong Lan Hoang0Alessandro Castorina1Yuen Yee Cheng2Quan Van Vuong3School of Environmental and Life Sciences, College of Engineering, Science and Environment, The University of Newcastle, Ourimbah, AustraliaLaboratory of Cellular and Molecular Neuroscience (LCMN), School of Life Sciences, Faculty of Science, University of Technology Sydney, Broadway, AustraliaInstitute for Biomedical Materials and Devices, University of Technology Sydney, Broadway, AustraliaSchool of Environmental and Life Sciences, College of Engineering, Science and Environment, The University of Newcastle, Ourimbah, AustraliaThe lemon-scented tea tree (Leptospermum petersonii) is rich in bioactive compounds with therapeutic potential; however, optimal extraction conditions for these compounds remain undefined. This study systematically optimised reflux extraction for 16 key bioactives from L. petersonii leaves. Solvent type and extraction conditions significantly influenced extraction yields, with 60% ethanol identified as the most effective solvent. Response surface methodology robustly modelled the effects of temperature, reflux time, and sample-to-solvent ratio (lack of fit = 0.1773; p = 0.0069; R² = 0.96). The predicted optimal conditions (95 °C, 160 min, 6:100 g/mL) yielded 39 mg/g of total bioactives. For improved cost-efficiency, extraction at 95 °C for 180 min with a 10:100 g/mL ratio was recommended, reducing solvent use by 40% with only an 8% yield loss, producing 35.8 mg/g bioactives. This extract exhibited strong cytotoxicity against prostate cancer (LNCaP), melanoma (A375, COLO 679), and mesothelioma (AC29) cell lines, with IC₅。 values < 2.50 μg/mL, while showing reduced toxicity toward non-cancerous BV2 cells. These findings establish efficient, scalable extraction conditions and highlight the anticancer potential of L. petersonii, warranting further isolation and mechanistic studies of its active constituents.https://www.tandfonline.com/doi/10.1080/23311932.2025.2544962Phenolic compoundsextractionleptospermumresponse surface methodologyanti-cancerFood Chemistry |
| spellingShingle | Phuong Lan Hoang Alessandro Castorina Yuen Yee Cheng Quan Van Vuong Optimum heat reflux extraction of key bioactive compounds from lemon scented tea tree (Leptospermum petersonii) leaves and their cytotoxic effects Cogent Food & Agriculture Phenolic compounds extraction leptospermum response surface methodology anti-cancer Food Chemistry |
| title | Optimum heat reflux extraction of key bioactive compounds from lemon scented tea tree (Leptospermum petersonii) leaves and their cytotoxic effects |
| title_full | Optimum heat reflux extraction of key bioactive compounds from lemon scented tea tree (Leptospermum petersonii) leaves and their cytotoxic effects |
| title_fullStr | Optimum heat reflux extraction of key bioactive compounds from lemon scented tea tree (Leptospermum petersonii) leaves and their cytotoxic effects |
| title_full_unstemmed | Optimum heat reflux extraction of key bioactive compounds from lemon scented tea tree (Leptospermum petersonii) leaves and their cytotoxic effects |
| title_short | Optimum heat reflux extraction of key bioactive compounds from lemon scented tea tree (Leptospermum petersonii) leaves and their cytotoxic effects |
| title_sort | optimum heat reflux extraction of key bioactive compounds from lemon scented tea tree leptospermum petersonii leaves and their cytotoxic effects |
| topic | Phenolic compounds extraction leptospermum response surface methodology anti-cancer Food Chemistry |
| url | https://www.tandfonline.com/doi/10.1080/23311932.2025.2544962 |
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