Constitutive Expression of Adiponectin in Endothelial Progenitor Cells Protects a Rat Model of Cerebral Ischemia
Endothelial progenitor cells (EPCs), as precursors to endothelial cells, play a significant part in the process of endogenous blood vessel repair and maintenance of endothelial integrity. Adiponectin (APN) is an adipocyte-specific adipocytokine. In this study, we aim to test whether we transplant a...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2017/6809745 |
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| author | Renwei Zhang Xiaorui Xie Qing Yu Hongliang Feng Meiyao Wang Yan Li Yumin Liu |
| author_facet | Renwei Zhang Xiaorui Xie Qing Yu Hongliang Feng Meiyao Wang Yan Li Yumin Liu |
| author_sort | Renwei Zhang |
| collection | DOAJ |
| description | Endothelial progenitor cells (EPCs), as precursors to endothelial cells, play a significant part in the process of endogenous blood vessel repair and maintenance of endothelial integrity. Adiponectin (APN) is an adipocyte-specific adipocytokine. In this study, we aim to test whether we transplant a combined graft of EPCs transfected with the adiponectin gene into a rat model of cerebral ischemia could improve functional recovery after middle cerebral artery occlusion (MCAO). Sprague-Dawley (SD) rats were randomly divided into a MCAO control group, a MCAO EPC treatment group, and a MCAO LV-APN-EPC treatment group. A focal cerebral ischemia and reperfusion model was induced by the intraluminal suture method. After 2 h of reperfusion, EPCs were transplanted by injection through the tail vein. A rotarod test was conducted to assess behavioral function before MCAO and on days 1, 7, and 14 after MCAO. After 14 d, TTC staining, CD31 immunofluorescence, and TUNEL staining were used to evaluate infarct volume, microvessel density, and cell apoptosis. Results revealed that behavioral function, infarct area percentage, microvessel density, and cell apoptosis rates were more favorable in the LV-APN-EPC treatment group than in the EPC treatment group. These data suggested that gene-modified cell therapy may be a useful approach for the treatment of ischemic stroke. |
| format | Article |
| id | doaj-art-2e11204ebeba44fba1fd1de935b2bd37 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-2e11204ebeba44fba1fd1de935b2bd372025-08-20T03:55:07ZengWileyNeural Plasticity2090-59041687-54432017-01-01201710.1155/2017/68097456809745Constitutive Expression of Adiponectin in Endothelial Progenitor Cells Protects a Rat Model of Cerebral IschemiaRenwei Zhang0Xiaorui Xie1Qing Yu2Hongliang Feng3Meiyao Wang4Yan Li5Yumin Liu6Department of Neurology, Zhongnan Hospital of Wuhan University, Wuhan 430071, ChinaDepartment of Neurology, Xiangyang Central Hospital, Xiangyang 441000, ChinaDepartment of Neurology, Zhongnan Hospital of Wuhan University, Wuhan 430071, ChinaDepartment of Neurology, Zhongnan Hospital of Wuhan University, Wuhan 430071, ChinaDepartment of Neurology, Zhongnan Hospital of Wuhan University, Wuhan 430071, ChinaDepartment of Neurology, Zhongnan Hospital of Wuhan University, Wuhan 430071, ChinaDepartment of Neurology, Zhongnan Hospital of Wuhan University, Wuhan 430071, ChinaEndothelial progenitor cells (EPCs), as precursors to endothelial cells, play a significant part in the process of endogenous blood vessel repair and maintenance of endothelial integrity. Adiponectin (APN) is an adipocyte-specific adipocytokine. In this study, we aim to test whether we transplant a combined graft of EPCs transfected with the adiponectin gene into a rat model of cerebral ischemia could improve functional recovery after middle cerebral artery occlusion (MCAO). Sprague-Dawley (SD) rats were randomly divided into a MCAO control group, a MCAO EPC treatment group, and a MCAO LV-APN-EPC treatment group. A focal cerebral ischemia and reperfusion model was induced by the intraluminal suture method. After 2 h of reperfusion, EPCs were transplanted by injection through the tail vein. A rotarod test was conducted to assess behavioral function before MCAO and on days 1, 7, and 14 after MCAO. After 14 d, TTC staining, CD31 immunofluorescence, and TUNEL staining were used to evaluate infarct volume, microvessel density, and cell apoptosis. Results revealed that behavioral function, infarct area percentage, microvessel density, and cell apoptosis rates were more favorable in the LV-APN-EPC treatment group than in the EPC treatment group. These data suggested that gene-modified cell therapy may be a useful approach for the treatment of ischemic stroke.http://dx.doi.org/10.1155/2017/6809745 |
| spellingShingle | Renwei Zhang Xiaorui Xie Qing Yu Hongliang Feng Meiyao Wang Yan Li Yumin Liu Constitutive Expression of Adiponectin in Endothelial Progenitor Cells Protects a Rat Model of Cerebral Ischemia Neural Plasticity |
| title | Constitutive Expression of Adiponectin in Endothelial Progenitor Cells Protects a Rat Model of Cerebral Ischemia |
| title_full | Constitutive Expression of Adiponectin in Endothelial Progenitor Cells Protects a Rat Model of Cerebral Ischemia |
| title_fullStr | Constitutive Expression of Adiponectin in Endothelial Progenitor Cells Protects a Rat Model of Cerebral Ischemia |
| title_full_unstemmed | Constitutive Expression of Adiponectin in Endothelial Progenitor Cells Protects a Rat Model of Cerebral Ischemia |
| title_short | Constitutive Expression of Adiponectin in Endothelial Progenitor Cells Protects a Rat Model of Cerebral Ischemia |
| title_sort | constitutive expression of adiponectin in endothelial progenitor cells protects a rat model of cerebral ischemia |
| url | http://dx.doi.org/10.1155/2017/6809745 |
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