PVA and PVP nanofibers combined with Helichrysum italicum oil preserve skin cell interactions, elasticity and proliferation
Abstract Development of electrospun nanofibers with suitable properties to promote wound healing is an advantage in developing non-invasive skin treatments. We showed the potential application of Polyvinyl acetate (PVA) and Polyvinylpyrrolidone (PVP) combined with Helichrysum italicum oil (HO) in wo...
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Nature Portfolio
2025-03-01
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| Online Access: | https://doi.org/10.1038/s41598-025-95788-z |
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| author | Diletta Serra Giuseppe Garroni Sara Cruciani Donatella Coradduzza Aleksei Pashchenko Evzen Amler Giorgio Pintore Pietro Parisse Rosanna Satta Fernanda Martini Mauro Tognon Antonio Brunetti Carlo Ventura Margherita Maioli |
| author_facet | Diletta Serra Giuseppe Garroni Sara Cruciani Donatella Coradduzza Aleksei Pashchenko Evzen Amler Giorgio Pintore Pietro Parisse Rosanna Satta Fernanda Martini Mauro Tognon Antonio Brunetti Carlo Ventura Margherita Maioli |
| author_sort | Diletta Serra |
| collection | DOAJ |
| description | Abstract Development of electrospun nanofibers with suitable properties to promote wound healing is an advantage in developing non-invasive skin treatments. We showed the potential application of Polyvinyl acetate (PVA) and Polyvinylpyrrolidone (PVP) combined with Helichrysum italicum oil (HO) in wound healing. During this process, Tight junctions (TJs) play a crucial role in maintaining skin integrity. TJs are intercellular junctions composed of a variety of transmembrane proteins, including Occludin (OCLN), observed also in migrating epithelial cells. Changes in OCLN expression affect epidermal permeability, indicating an active role in the healing process. Within this context, we studied the OCLN expression during healing after scratch assay on Keratinocytes (HaCaT), by a confocal microscopic analysis. In addition, we evaluated the effect of treatment after scratch on cell elasticity by Atomic Force Microscopy (AFM) analysis. All results show a positive trend in cell proliferation and viability on HaCaT treated with functionalized nanofibers. These results were confirmed by the expression of genes involved in the early stages of the regenerative process. Understanding the cell mechanisms involved in skin changes during repair process would allow future application of nanomaterials combined with HO in vivo. |
| format | Article |
| id | doaj-art-2e018dbf324b473c8a45d22683690da1 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-2e018dbf324b473c8a45d22683690da12025-08-20T02:49:32ZengNature PortfolioScientific Reports2045-23222025-03-011511910.1038/s41598-025-95788-zPVA and PVP nanofibers combined with Helichrysum italicum oil preserve skin cell interactions, elasticity and proliferationDiletta Serra0Giuseppe Garroni1Sara Cruciani2Donatella Coradduzza3Aleksei Pashchenko4Evzen Amler5Giorgio Pintore6Pietro Parisse7Rosanna Satta8Fernanda Martini9Mauro Tognon10Antonio Brunetti11Carlo Ventura12Margherita Maioli13Department of Biomedical Sciences, University of SassariDepartment of Biomedical Sciences, University of SassariDepartment of Biomedical Sciences, University of SassariDepartment of Biomedical Sciences, University of SassariDepartment of Biomedical Sciences, University of SassariUniversity Centre for Energy Efficient Buildings, Czech Technical University in PragueDepartment of Medicine, Surgery and Pharmacy, University of SassariInstitute of Materials (IOM-CNR)Department of Medicine, Surgery and Pharmacy, University of SassariDepartment of Medical Sciences, University of FerraraDepartment of Medical Sciences, University of FerraraDepartment of Biomedical Sciences, University of SassariLaboratory of Molecular Biology and Stem Cell Engineering – Eldor Lab Istituto Nazionale Biostrutture e BiosistemiDepartment of Biomedical Sciences, University of SassariAbstract Development of electrospun nanofibers with suitable properties to promote wound healing is an advantage in developing non-invasive skin treatments. We showed the potential application of Polyvinyl acetate (PVA) and Polyvinylpyrrolidone (PVP) combined with Helichrysum italicum oil (HO) in wound healing. During this process, Tight junctions (TJs) play a crucial role in maintaining skin integrity. TJs are intercellular junctions composed of a variety of transmembrane proteins, including Occludin (OCLN), observed also in migrating epithelial cells. Changes in OCLN expression affect epidermal permeability, indicating an active role in the healing process. Within this context, we studied the OCLN expression during healing after scratch assay on Keratinocytes (HaCaT), by a confocal microscopic analysis. In addition, we evaluated the effect of treatment after scratch on cell elasticity by Atomic Force Microscopy (AFM) analysis. All results show a positive trend in cell proliferation and viability on HaCaT treated with functionalized nanofibers. These results were confirmed by the expression of genes involved in the early stages of the regenerative process. Understanding the cell mechanisms involved in skin changes during repair process would allow future application of nanomaterials combined with HO in vivo.https://doi.org/10.1038/s41598-025-95788-zKeratinocytesNanofibersRegenerative medicineTissue regenerationWound healingCellular mechanism |
| spellingShingle | Diletta Serra Giuseppe Garroni Sara Cruciani Donatella Coradduzza Aleksei Pashchenko Evzen Amler Giorgio Pintore Pietro Parisse Rosanna Satta Fernanda Martini Mauro Tognon Antonio Brunetti Carlo Ventura Margherita Maioli PVA and PVP nanofibers combined with Helichrysum italicum oil preserve skin cell interactions, elasticity and proliferation Scientific Reports Keratinocytes Nanofibers Regenerative medicine Tissue regeneration Wound healing Cellular mechanism |
| title | PVA and PVP nanofibers combined with Helichrysum italicum oil preserve skin cell interactions, elasticity and proliferation |
| title_full | PVA and PVP nanofibers combined with Helichrysum italicum oil preserve skin cell interactions, elasticity and proliferation |
| title_fullStr | PVA and PVP nanofibers combined with Helichrysum italicum oil preserve skin cell interactions, elasticity and proliferation |
| title_full_unstemmed | PVA and PVP nanofibers combined with Helichrysum italicum oil preserve skin cell interactions, elasticity and proliferation |
| title_short | PVA and PVP nanofibers combined with Helichrysum italicum oil preserve skin cell interactions, elasticity and proliferation |
| title_sort | pva and pvp nanofibers combined with helichrysum italicum oil preserve skin cell interactions elasticity and proliferation |
| topic | Keratinocytes Nanofibers Regenerative medicine Tissue regeneration Wound healing Cellular mechanism |
| url | https://doi.org/10.1038/s41598-025-95788-z |
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