Effect of The Duration of The Diagnosis on DNA Methylation Changes in Three Different Diabetic Genes in Type 2 Diabetes Mellitus
The primary epigenetic alteration is DNA methylation. This study examined variations in DNA methylation in patients with type 2 diabetes at various time intervals, concentrating on the Calpain-10 (CAPN10), ATP binding cassette subfamily C member 8 (ABCC8), and Transcription factor 7-like 2 (TCF7L...
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Sulaimani Polytechnic University
2024-11-01
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| Series: | Kurdistan Journal of Applied Research |
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| Online Access: | https://portal.spu.edu.iq/index.php/kjar/article/view/968 |
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| author | Harem Othman Smail |
| author_facet | Harem Othman Smail |
| author_sort | Harem Othman Smail |
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The primary epigenetic alteration is DNA methylation. This study examined variations in DNA methylation in patients with type 2 diabetes at various time intervals, concentrating on the Calpain-10 (CAPN10), ATP binding cassette subfamily C member 8 (ABCC8), and Transcription factor 7-like 2 (TCF7L2) genes. Since persistent diabetes can lead to aberrant methylation patterns, it is interested in how long these changes may last. The blood samples from 140 individuals with Type 2 Diabetes Mellitus (T2DM) were collected, and the patients were grouped according to how long they had been diagnosed. Four groups of individuals were created based on their time on the disease: those with T2DM <1 year, those with T2DM 1-3 years, and those with T2DM 3-5 years. The genders and ages of the participants were also noted. Using the Promega technique, bisulfite conversion and DNA extraction were completed. Methylation-specific PCR amplification was used to detect DNA methylation. In the study, ROC curve analysis, Chi-square and Spearman's correlation coefficients, as well as non-parametric tests, were employed to analyze the methylation percentage variation and methylation patterns among groups. A significant threshold of p < 0.05 was established. The results of the study indicated that the DNA methylation rates of T2DM patients and the control group differed significantly. Patients with type 2 diabetes, particularly those who had just received a diagnosis, showed higher levels of methylation than the control group. The study also found that the length of diagnosis may have an impact on the discriminative strength of the DNA methylation status of the ABCC8 gene, resulting in varying degrees of T2DM prediction.
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| format | Article |
| id | doaj-art-2dfa1a0205614e4e8590cb5a22142b41 |
| institution | Kabale University |
| issn | 2411-7684 2411-7706 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Sulaimani Polytechnic University |
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| series | Kurdistan Journal of Applied Research |
| spelling | doaj-art-2dfa1a0205614e4e8590cb5a22142b412025-02-07T20:58:52ZengSulaimani Polytechnic UniversityKurdistan Journal of Applied Research2411-76842411-77062024-11-019210.24017/science.2024.2.7Effect of The Duration of The Diagnosis on DNA Methylation Changes in Three Different Diabetic Genes in Type 2 Diabetes MellitusHarem Othman Smail0https://orcid.org/0000-0001-9867-4289Department of Biology, Faculty of Science and Health, Koya University, Koya , Iraq The primary epigenetic alteration is DNA methylation. This study examined variations in DNA methylation in patients with type 2 diabetes at various time intervals, concentrating on the Calpain-10 (CAPN10), ATP binding cassette subfamily C member 8 (ABCC8), and Transcription factor 7-like 2 (TCF7L2) genes. Since persistent diabetes can lead to aberrant methylation patterns, it is interested in how long these changes may last. The blood samples from 140 individuals with Type 2 Diabetes Mellitus (T2DM) were collected, and the patients were grouped according to how long they had been diagnosed. Four groups of individuals were created based on their time on the disease: those with T2DM <1 year, those with T2DM 1-3 years, and those with T2DM 3-5 years. The genders and ages of the participants were also noted. Using the Promega technique, bisulfite conversion and DNA extraction were completed. Methylation-specific PCR amplification was used to detect DNA methylation. In the study, ROC curve analysis, Chi-square and Spearman's correlation coefficients, as well as non-parametric tests, were employed to analyze the methylation percentage variation and methylation patterns among groups. A significant threshold of p < 0.05 was established. The results of the study indicated that the DNA methylation rates of T2DM patients and the control group differed significantly. Patients with type 2 diabetes, particularly those who had just received a diagnosis, showed higher levels of methylation than the control group. The study also found that the length of diagnosis may have an impact on the discriminative strength of the DNA methylation status of the ABCC8 gene, resulting in varying degrees of T2DM prediction. https://portal.spu.edu.iq/index.php/kjar/article/view/968DNA methylationtype 2 diabetesmethylation-specific PCRbisulfite conversion and diag-nosis times |
| spellingShingle | Harem Othman Smail Effect of The Duration of The Diagnosis on DNA Methylation Changes in Three Different Diabetic Genes in Type 2 Diabetes Mellitus Kurdistan Journal of Applied Research DNA methylation type 2 diabetes methylation-specific PCR bisulfite conversion and diag-nosis times |
| title | Effect of The Duration of The Diagnosis on DNA Methylation Changes in Three Different Diabetic Genes in Type 2 Diabetes Mellitus |
| title_full | Effect of The Duration of The Diagnosis on DNA Methylation Changes in Three Different Diabetic Genes in Type 2 Diabetes Mellitus |
| title_fullStr | Effect of The Duration of The Diagnosis on DNA Methylation Changes in Three Different Diabetic Genes in Type 2 Diabetes Mellitus |
| title_full_unstemmed | Effect of The Duration of The Diagnosis on DNA Methylation Changes in Three Different Diabetic Genes in Type 2 Diabetes Mellitus |
| title_short | Effect of The Duration of The Diagnosis on DNA Methylation Changes in Three Different Diabetic Genes in Type 2 Diabetes Mellitus |
| title_sort | effect of the duration of the diagnosis on dna methylation changes in three different diabetic genes in type 2 diabetes mellitus |
| topic | DNA methylation type 2 diabetes methylation-specific PCR bisulfite conversion and diag-nosis times |
| url | https://portal.spu.edu.iq/index.php/kjar/article/view/968 |
| work_keys_str_mv | AT haremothmansmail effectofthedurationofthediagnosisondnamethylationchangesinthreedifferentdiabeticgenesintype2diabetesmellitus |