New Insights into the Immunological Changes in IL-10-Deficient Mice during the Course of Spontaneous Inflammation in the Gut Mucosa
IL-10 is a regulatory cytokine that plays a major role in the homeostasis of the gut and this is illustrated by the fact that IL-10−/− mice develop spontaneous colitis. In this study, IL-10−/− mice were analyzed for immunological changes during colitis development. We found a reduced frequency of re...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/560817 |
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| author | Ana Cristina Gomes-Santos Thais Garcias Moreira Archimedes Barbosa Castro-Junior Bernardo Coelho Horta Luisa Lemos Deborah Nogueira Cruz Mauro Andrade Freitas Guimarães Denise Carmona Cara Donna-Marie McCafferty Ana Maria Caetano Faria |
| author_facet | Ana Cristina Gomes-Santos Thais Garcias Moreira Archimedes Barbosa Castro-Junior Bernardo Coelho Horta Luisa Lemos Deborah Nogueira Cruz Mauro Andrade Freitas Guimarães Denise Carmona Cara Donna-Marie McCafferty Ana Maria Caetano Faria |
| author_sort | Ana Cristina Gomes-Santos |
| collection | DOAJ |
| description | IL-10 is a regulatory cytokine that plays a major role in the homeostasis of the gut and this is illustrated by the fact that IL-10−/− mice develop spontaneous colitis. In this study, IL-10−/− mice were analyzed for immunological changes during colitis development. We found a reduced frequency of regulatory T cells CD4+CD25+Foxp3+ and higher frequency of activated T cells in the colon that precedes the macroscopic signs of the disease. Production of IL-17 and IFN-γ was higher in the colon. Colitis progression culminates with the reduction of CD4+LAP+ regulatory T cells in the intestine. Frequency of B1 cells and the secretory IgA production were both elevated. Despite these alterations, 16-week-old IL-10−/− mice could be rendered tolerant by a continuous feeding protocol. Our study provides detailed analysis of changes that precede colitis and it also suggests that oral tolerance could be used to design novel alternative therapies for the disease. |
| format | Article |
| id | doaj-art-2df6e7d9d0f448ca97b60e3d9d39e44e |
| institution | DOAJ |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-2df6e7d9d0f448ca97b60e3d9d39e44e2025-08-20T03:20:26ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/560817560817New Insights into the Immunological Changes in IL-10-Deficient Mice during the Course of Spontaneous Inflammation in the Gut MucosaAna Cristina Gomes-Santos0Thais Garcias Moreira1Archimedes Barbosa Castro-Junior2Bernardo Coelho Horta3Luisa Lemos4Deborah Nogueira Cruz5Mauro Andrade Freitas Guimarães6Denise Carmona Cara7Donna-Marie McCafferty8Ana Maria Caetano Faria9Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, BrazilDepartamento de Ciência de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BrazilDepartamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, BrazilDepartamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, BrazilDepartamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, BrazilDepartamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, BrazilDepartamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, BrazilDepartamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BrazilGastrointestinal Research Group, Department of Physiology and Pharmacology, University of Calgary, 2500 University Dr. NW T2N 4N1, Calgary, AB, CanadaDepartamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, BrazilIL-10 is a regulatory cytokine that plays a major role in the homeostasis of the gut and this is illustrated by the fact that IL-10−/− mice develop spontaneous colitis. In this study, IL-10−/− mice were analyzed for immunological changes during colitis development. We found a reduced frequency of regulatory T cells CD4+CD25+Foxp3+ and higher frequency of activated T cells in the colon that precedes the macroscopic signs of the disease. Production of IL-17 and IFN-γ was higher in the colon. Colitis progression culminates with the reduction of CD4+LAP+ regulatory T cells in the intestine. Frequency of B1 cells and the secretory IgA production were both elevated. Despite these alterations, 16-week-old IL-10−/− mice could be rendered tolerant by a continuous feeding protocol. Our study provides detailed analysis of changes that precede colitis and it also suggests that oral tolerance could be used to design novel alternative therapies for the disease.http://dx.doi.org/10.1155/2012/560817 |
| spellingShingle | Ana Cristina Gomes-Santos Thais Garcias Moreira Archimedes Barbosa Castro-Junior Bernardo Coelho Horta Luisa Lemos Deborah Nogueira Cruz Mauro Andrade Freitas Guimarães Denise Carmona Cara Donna-Marie McCafferty Ana Maria Caetano Faria New Insights into the Immunological Changes in IL-10-Deficient Mice during the Course of Spontaneous Inflammation in the Gut Mucosa Clinical and Developmental Immunology |
| title | New Insights into the Immunological Changes in IL-10-Deficient Mice during the Course of Spontaneous Inflammation in the Gut Mucosa |
| title_full | New Insights into the Immunological Changes in IL-10-Deficient Mice during the Course of Spontaneous Inflammation in the Gut Mucosa |
| title_fullStr | New Insights into the Immunological Changes in IL-10-Deficient Mice during the Course of Spontaneous Inflammation in the Gut Mucosa |
| title_full_unstemmed | New Insights into the Immunological Changes in IL-10-Deficient Mice during the Course of Spontaneous Inflammation in the Gut Mucosa |
| title_short | New Insights into the Immunological Changes in IL-10-Deficient Mice during the Course of Spontaneous Inflammation in the Gut Mucosa |
| title_sort | new insights into the immunological changes in il 10 deficient mice during the course of spontaneous inflammation in the gut mucosa |
| url | http://dx.doi.org/10.1155/2012/560817 |
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