Identification of Coxiella burnetii in Raw Milk of Livestock Animal in Iran

Coxiella burnetii is the causative agent of Q fever in humans and animals. This study aimed to determine the frequency of C. burnetii in milk samples of dairy animals (goats, sheep, and cattle) in some selected regions in Iran, where there is no information about prevalence of C. burnetii. In this s...

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Main Authors: Ashraf Mohabati Mobarez, Ehsan Mostafavi, Mohammad Khalili, Saber Esmaeili
Format: Article
Language:English
Published: Wiley 2021-01-01
Series:International Journal of Microbiology
Online Access:http://dx.doi.org/10.1155/2021/6632036
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author Ashraf Mohabati Mobarez
Ehsan Mostafavi
Mohammad Khalili
Saber Esmaeili
author_facet Ashraf Mohabati Mobarez
Ehsan Mostafavi
Mohammad Khalili
Saber Esmaeili
author_sort Ashraf Mohabati Mobarez
collection DOAJ
description Coxiella burnetii is the causative agent of Q fever in humans and animals. This study aimed to determine the frequency of C. burnetii in milk samples of dairy animals (goats, sheep, and cattle) in some selected regions in Iran, where there is no information about prevalence of C. burnetii. In this study, 162 individual milk samples were collected from 43 farms in three provinces (Tehran, Hamadan, and Mazandaran). Real-time PCR was used for the detection of IS1111a element of C. burnetii. In total, 23 of 162 samples (14.2%, 95% confidence interval (CI): 9.65–20.2%) were positive for C. burnetii by real-time PCR. C. burnetii was detected in 10.17% (95% CI: 4.74–20.46) of goat milk samples. In sheep milk samples, 18.6% (95% CI: 9.74–32.62) were positive, and C. burnetii was detected in 15% (95% CI: 8.1–26.11) of cattle milk samples. Molecular evidence of the presence of C. burnetii was seen in milk samples of dairy animals in all the studied regions. These findings demonstrated that C. burnetii infection, especially in raw milk samples, deserves more attention from the health care system and veterinary organization in Iran.
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spelling doaj-art-2df2909dbfab448cbe65575bdb520f1c2025-08-20T03:20:26ZengWileyInternational Journal of Microbiology1687-918X1687-91982021-01-01202110.1155/2021/66320366632036Identification of Coxiella burnetii in Raw Milk of Livestock Animal in IranAshraf Mohabati Mobarez0Ehsan Mostafavi1Mohammad Khalili2Saber Esmaeili3Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Epidemiology and Biostatics, Pasteur Institute of Iran, Tehran, IranDepartment of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, IranDepartment of Epidemiology and Biostatics, Pasteur Institute of Iran, Tehran, IranCoxiella burnetii is the causative agent of Q fever in humans and animals. This study aimed to determine the frequency of C. burnetii in milk samples of dairy animals (goats, sheep, and cattle) in some selected regions in Iran, where there is no information about prevalence of C. burnetii. In this study, 162 individual milk samples were collected from 43 farms in three provinces (Tehran, Hamadan, and Mazandaran). Real-time PCR was used for the detection of IS1111a element of C. burnetii. In total, 23 of 162 samples (14.2%, 95% confidence interval (CI): 9.65–20.2%) were positive for C. burnetii by real-time PCR. C. burnetii was detected in 10.17% (95% CI: 4.74–20.46) of goat milk samples. In sheep milk samples, 18.6% (95% CI: 9.74–32.62) were positive, and C. burnetii was detected in 15% (95% CI: 8.1–26.11) of cattle milk samples. Molecular evidence of the presence of C. burnetii was seen in milk samples of dairy animals in all the studied regions. These findings demonstrated that C. burnetii infection, especially in raw milk samples, deserves more attention from the health care system and veterinary organization in Iran.http://dx.doi.org/10.1155/2021/6632036
spellingShingle Ashraf Mohabati Mobarez
Ehsan Mostafavi
Mohammad Khalili
Saber Esmaeili
Identification of Coxiella burnetii in Raw Milk of Livestock Animal in Iran
International Journal of Microbiology
title Identification of Coxiella burnetii in Raw Milk of Livestock Animal in Iran
title_full Identification of Coxiella burnetii in Raw Milk of Livestock Animal in Iran
title_fullStr Identification of Coxiella burnetii in Raw Milk of Livestock Animal in Iran
title_full_unstemmed Identification of Coxiella burnetii in Raw Milk of Livestock Animal in Iran
title_short Identification of Coxiella burnetii in Raw Milk of Livestock Animal in Iran
title_sort identification of coxiella burnetii in raw milk of livestock animal in iran
url http://dx.doi.org/10.1155/2021/6632036
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