Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes
Recent advances in the stem cell field allow to obtain many human tissues in vitro. However, hepatic differentiation of induced pluripotent stem cells (iPSCs) still remains challenging. Hepatocyte-like cells (HLCs) obtained after differentiation resemble more fetal liver hepatocytes. MicroRNAs (miRN...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/5692840 |
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| _version_ | 1832563549488021504 |
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| author | Aniela Skrzypczyk Stephanie Kehr Ilona Krystel Stephan H. Bernhart Shibashish Giri Augustinus Bader Peter F. Stadler |
| author_facet | Aniela Skrzypczyk Stephanie Kehr Ilona Krystel Stephan H. Bernhart Shibashish Giri Augustinus Bader Peter F. Stadler |
| author_sort | Aniela Skrzypczyk |
| collection | DOAJ |
| description | Recent advances in the stem cell field allow to obtain many human tissues in vitro. However, hepatic differentiation of induced pluripotent stem cells (iPSCs) still remains challenging. Hepatocyte-like cells (HLCs) obtained after differentiation resemble more fetal liver hepatocytes. MicroRNAs (miRNA) play an important role in the differentiation process. Here, we analysed noncoding RNA profiles from the last stages of differentiation and compare them to hepatocytes. Our results show that HLCs maintain an epithelial character and express miRNA which can block hepatocyte maturation by inhibiting the epithelial-mesenchymal transition (EMT). Additionally, we identified differentially expressed small nucleolar RNAs (snoRNAs) and discovered novel noncoding RNA (ncRNA) genes. |
| format | Article |
| id | doaj-art-2def41076f034a18bfa9fcefeef1fe2d |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-2def41076f034a18bfa9fcefeef1fe2d2025-02-03T01:13:13ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/56928405692840Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into HepatocytesAniela Skrzypczyk0Stephanie Kehr1Ilona Krystel2Stephan H. Bernhart3Shibashish Giri4Augustinus Bader5Peter F. Stadler6Applied Stem Cell Biology and Cell Technology, Biomedical and Biotechnological Center, Leipzig University, Deutscher Platz 5, 04103 Leipzig, GermanyBioinformatics Group, Department of Computer Science, and Interdisciplinary Centre for Bioinformatics, Leipzig University, Härtelstr. 1618, 04107 Leipzig, GermanyApplied Stem Cell Biology and Cell Technology, Biomedical and Biotechnological Center, Leipzig University, Deutscher Platz 5, 04103 Leipzig, GermanyBioinformatics Group, Department of Computer Science, and Interdisciplinary Centre for Bioinformatics, Leipzig University, Härtelstr. 1618, 04107 Leipzig, GermanyApplied Stem Cell Biology and Cell Technology, Biomedical and Biotechnological Center, Leipzig University, Deutscher Platz 5, 04103 Leipzig, GermanyApplied Stem Cell Biology and Cell Technology, Biomedical and Biotechnological Center, Leipzig University, Deutscher Platz 5, 04103 Leipzig, GermanyBioinformatics Group, Department of Computer Science, and Interdisciplinary Centre for Bioinformatics, Leipzig University, Härtelstr. 1618, 04107 Leipzig, GermanyRecent advances in the stem cell field allow to obtain many human tissues in vitro. However, hepatic differentiation of induced pluripotent stem cells (iPSCs) still remains challenging. Hepatocyte-like cells (HLCs) obtained after differentiation resemble more fetal liver hepatocytes. MicroRNAs (miRNA) play an important role in the differentiation process. Here, we analysed noncoding RNA profiles from the last stages of differentiation and compare them to hepatocytes. Our results show that HLCs maintain an epithelial character and express miRNA which can block hepatocyte maturation by inhibiting the epithelial-mesenchymal transition (EMT). Additionally, we identified differentially expressed small nucleolar RNAs (snoRNAs) and discovered novel noncoding RNA (ncRNA) genes.http://dx.doi.org/10.1155/2018/5692840 |
| spellingShingle | Aniela Skrzypczyk Stephanie Kehr Ilona Krystel Stephan H. Bernhart Shibashish Giri Augustinus Bader Peter F. Stadler Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes Stem Cells International |
| title | Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes |
| title_full | Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes |
| title_fullStr | Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes |
| title_full_unstemmed | Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes |
| title_short | Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes |
| title_sort | noncoding rna transcripts during differentiation of induced pluripotent stem cells into hepatocytes |
| url | http://dx.doi.org/10.1155/2018/5692840 |
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