Genomic Insight into <i>Vibrio</i> Isolates from Fresh Raw Mussels and Ready-to-Eat Stuffed Mussels
Consuming raw or undercooked mussels can lead to gastroenteritis and septicemia due to <i>Vibrio</i> contamination. This study analyzed the prevalence, density, species diversity, and molecular traits of <i>Vibrio</i> spp. in 48 fresh raw wild mussels (FRMs) and 48 ready-to-e...
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2025-01-01
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| author | Artun Yibar Muhammed Duman Hilal Ay Nihed Ajmi Gorkem Tasci Fatma Gurler Sabire Guler Danny Morick Izzet Burcin Saticioglu |
| author_facet | Artun Yibar Muhammed Duman Hilal Ay Nihed Ajmi Gorkem Tasci Fatma Gurler Sabire Guler Danny Morick Izzet Burcin Saticioglu |
| author_sort | Artun Yibar |
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| description | Consuming raw or undercooked mussels can lead to gastroenteritis and septicemia due to <i>Vibrio</i> contamination. This study analyzed the prevalence, density, species diversity, and molecular traits of <i>Vibrio</i> spp. in 48 fresh raw wild mussels (FRMs) and 48 ready-to-eat stuffed mussels (RTE-SMs) through genome analysis, assessing health risks. The results showed <i>Vibrio</i> prevalence rates of 12.5% in FRMs and 4.2% in RTE-SMs, with <i>V. alginolyticus</i> as the most common species (46.7%). It was determined that the seasonal distribution of <i>Vibrio</i> spp. prevalence in the samples was higher in the summer months. The genome sizes of the <i>Vibrio</i> spp. ranged from approximately 3.9 to 6.1 Mb, with the GC contents varying between 41.9% and 50.4%. A total of 22 virulence factor (VF) classes and up to six antimicrobial resistance (AMR) genes were detected in different <i>Vibrio</i> species. The presence of nine different biosynthetic gene clusters (BGCs), 27 prophage regions, and eight CRISPR/Cas systems in 15 <i>Vibrio</i> strains provides information about their potential pathogenicity, survival strategies, and adaptation to different habitats. Overall, this study provides a comprehensive understanding of the genomic diversity of <i>Vibrio</i> spp. isolated from FRM and RTE-SM samples, shedding light on the prevalence, pathogenicity, and toxicity mechanisms of <i>Vibrio</i>-induced gastroenteritis. |
| format | Article |
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| institution | Kabale University |
| issn | 2076-0817 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Pathogens |
| spelling | doaj-art-2de66679d1074b4481003b0d531167232025-01-24T13:44:44ZengMDPI AGPathogens2076-08172025-01-011415210.3390/pathogens14010052Genomic Insight into <i>Vibrio</i> Isolates from Fresh Raw Mussels and Ready-to-Eat Stuffed MusselsArtun Yibar0Muhammed Duman1Hilal Ay2Nihed Ajmi3Gorkem Tasci4Fatma Gurler5Sabire Guler6Danny Morick7Izzet Burcin Saticioglu8Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa 16059, TürkiyeDepartment of Aquatic Animal Disease, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa 16059, TürkiyeDepartment of Molecular Biology and Genetics, Faculty of Arts and Science, Yildiz Technical University, Istanbul 34220, TürkiyeDepartment of Aquatic Animal Disease, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa 16059, TürkiyeDepartment of Aquatic Animal Disease, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa 16059, TürkiyeDepartment of Food Hygiene and Technology, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa 16059, TürkiyeDepartment of Histology and Embryology, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa 16059, TürkiyeDepartment of Blue Biotechnologies and Sustainable Mariculture, The Leon H. Charney School of Marine Sciences, University of Haifa, Haifa 3498838, IsraelDepartment of Aquatic Animal Disease, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa 16059, TürkiyeConsuming raw or undercooked mussels can lead to gastroenteritis and septicemia due to <i>Vibrio</i> contamination. This study analyzed the prevalence, density, species diversity, and molecular traits of <i>Vibrio</i> spp. in 48 fresh raw wild mussels (FRMs) and 48 ready-to-eat stuffed mussels (RTE-SMs) through genome analysis, assessing health risks. The results showed <i>Vibrio</i> prevalence rates of 12.5% in FRMs and 4.2% in RTE-SMs, with <i>V. alginolyticus</i> as the most common species (46.7%). It was determined that the seasonal distribution of <i>Vibrio</i> spp. prevalence in the samples was higher in the summer months. The genome sizes of the <i>Vibrio</i> spp. ranged from approximately 3.9 to 6.1 Mb, with the GC contents varying between 41.9% and 50.4%. A total of 22 virulence factor (VF) classes and up to six antimicrobial resistance (AMR) genes were detected in different <i>Vibrio</i> species. The presence of nine different biosynthetic gene clusters (BGCs), 27 prophage regions, and eight CRISPR/Cas systems in 15 <i>Vibrio</i> strains provides information about their potential pathogenicity, survival strategies, and adaptation to different habitats. Overall, this study provides a comprehensive understanding of the genomic diversity of <i>Vibrio</i> spp. isolated from FRM and RTE-SM samples, shedding light on the prevalence, pathogenicity, and toxicity mechanisms of <i>Vibrio</i>-induced gastroenteritis.https://www.mdpi.com/2076-0817/14/1/52whole-genome sequencingfoodborne pathogenmusselpublic health<i>Vibrio</i> spp. |
| spellingShingle | Artun Yibar Muhammed Duman Hilal Ay Nihed Ajmi Gorkem Tasci Fatma Gurler Sabire Guler Danny Morick Izzet Burcin Saticioglu Genomic Insight into <i>Vibrio</i> Isolates from Fresh Raw Mussels and Ready-to-Eat Stuffed Mussels Pathogens whole-genome sequencing foodborne pathogen mussel public health <i>Vibrio</i> spp. |
| title | Genomic Insight into <i>Vibrio</i> Isolates from Fresh Raw Mussels and Ready-to-Eat Stuffed Mussels |
| title_full | Genomic Insight into <i>Vibrio</i> Isolates from Fresh Raw Mussels and Ready-to-Eat Stuffed Mussels |
| title_fullStr | Genomic Insight into <i>Vibrio</i> Isolates from Fresh Raw Mussels and Ready-to-Eat Stuffed Mussels |
| title_full_unstemmed | Genomic Insight into <i>Vibrio</i> Isolates from Fresh Raw Mussels and Ready-to-Eat Stuffed Mussels |
| title_short | Genomic Insight into <i>Vibrio</i> Isolates from Fresh Raw Mussels and Ready-to-Eat Stuffed Mussels |
| title_sort | genomic insight into i vibrio i isolates from fresh raw mussels and ready to eat stuffed mussels |
| topic | whole-genome sequencing foodborne pathogen mussel public health <i>Vibrio</i> spp. |
| url | https://www.mdpi.com/2076-0817/14/1/52 |
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