Identification and Specific KASP Marker Development for Durum Wheat T2DS-2AS.2AL Translocation Line YL-429 with Wax Inhibitor Gene <i>IW2</i>
Non-glaucous wheat can reduce solar light reflection in low-light cultivation regions, enhancing photosynthetic efficiency and potentially increasing yield. In previous work, a non-glaucous cuticular line, YL-429, was discovered in derivatives of pentaploid hybrids by crossing the synthetic wheat LM...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/7/1077 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849730373217419264 |
|---|---|
| author | Sujie Yang Fan Yang Zujun Yang Wenjing Hu Hongxia Ding Feiyang Yang Hongshen Wan Zehou Liu Tao Lang Ning Yang Jie Zhang Yun Jiang Junyan Feng Hao Tang Qian Chen Qian Deng Ying Wang Jingyu Wu Jun Xiao Xing Fan Yonghong Zhou Jun Li |
| author_facet | Sujie Yang Fan Yang Zujun Yang Wenjing Hu Hongxia Ding Feiyang Yang Hongshen Wan Zehou Liu Tao Lang Ning Yang Jie Zhang Yun Jiang Junyan Feng Hao Tang Qian Chen Qian Deng Ying Wang Jingyu Wu Jun Xiao Xing Fan Yonghong Zhou Jun Li |
| author_sort | Sujie Yang |
| collection | DOAJ |
| description | Non-glaucous wheat can reduce solar light reflection in low-light cultivation regions, enhancing photosynthetic efficiency and potentially increasing yield. In previous work, a non-glaucous cuticular line, YL-429, was discovered in derivatives of pentaploid hybrids by crossing the synthetic wheat LM/AT23 (non-glaucous cuticular) with its tetraploid donor parent LM (glaucous) and selfing to F<sub>7</sub> generations. In the present study, multicolor fluorescence in situ hybridization was used to characterize the karyotype of the YL-429 line; genome resequencing was performed to identify the breakpoint of the 2D-2A chromosome translocation of YL-429; and bulk sequencing analysis was conducted to detect the SNP in the translocated fragment and accordingly develop specific kompetitive allele-specific PCR markers for use in breeding. The line YL-429 was preliminarily determined as a 2DS and 2AS translocation (LM T2DS-2AS.2AL) line through karyotyping. Genome alignment identified an approximately 13.8 Mb segment, including the wax inhibitor gene <i>Iw2</i>, in the telomeric region of the 2DS chromosome arm replacing an approximately 16.1 Mb segment in that of the 2AS chromosome arm. According to the bulk DNA sequencing data, 27 specific KASP markers were developed for detecting the translocated fragment from the 2DS of <i>Aegilops tauschii</i>. The LM T2DS-2AS.2AL translocation line YL-429 could be helpful in improving the photosynthesis of durum wheat cultivated in low-light cultivation regions. The developed markers can assist the screening of the T2DS-2AS.2AL translocation in breeding. |
| format | Article |
| id | doaj-art-2de374a2a0394ecda359ea9df9b915be |
| institution | DOAJ |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-2de374a2a0394ecda359ea9df9b915be2025-08-20T03:08:53ZengMDPI AGPlants2223-77472025-04-01147107710.3390/plants14071077Identification and Specific KASP Marker Development for Durum Wheat T2DS-2AS.2AL Translocation Line YL-429 with Wax Inhibitor Gene <i>IW2</i>Sujie Yang0Fan Yang1Zujun Yang2Wenjing Hu3Hongxia Ding4Feiyang Yang5Hongshen Wan6Zehou Liu7Tao Lang8Ning Yang9Jie Zhang10Yun Jiang11Junyan Feng12Hao Tang13Qian Chen14Qian Deng15Ying Wang16Jingyu Wu17Jun Xiao18Xing Fan19Yonghong Zhou20Jun Li21Biotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaBiotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaSchool of Life Science and Technology, University of Electronic Science and Technology, Chengdu 611731, ChinaLixiahe Institute of Agricultural Sciences, Yangzhou 225007, ChinaBiotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaBiotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaBiotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaBiotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaBiotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaBiotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaBiotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaBiotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaBiotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaBiotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaBiotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaNon-glaucous wheat can reduce solar light reflection in low-light cultivation regions, enhancing photosynthetic efficiency and potentially increasing yield. In previous work, a non-glaucous cuticular line, YL-429, was discovered in derivatives of pentaploid hybrids by crossing the synthetic wheat LM/AT23 (non-glaucous cuticular) with its tetraploid donor parent LM (glaucous) and selfing to F<sub>7</sub> generations. In the present study, multicolor fluorescence in situ hybridization was used to characterize the karyotype of the YL-429 line; genome resequencing was performed to identify the breakpoint of the 2D-2A chromosome translocation of YL-429; and bulk sequencing analysis was conducted to detect the SNP in the translocated fragment and accordingly develop specific kompetitive allele-specific PCR markers for use in breeding. The line YL-429 was preliminarily determined as a 2DS and 2AS translocation (LM T2DS-2AS.2AL) line through karyotyping. Genome alignment identified an approximately 13.8 Mb segment, including the wax inhibitor gene <i>Iw2</i>, in the telomeric region of the 2DS chromosome arm replacing an approximately 16.1 Mb segment in that of the 2AS chromosome arm. According to the bulk DNA sequencing data, 27 specific KASP markers were developed for detecting the translocated fragment from the 2DS of <i>Aegilops tauschii</i>. The LM T2DS-2AS.2AL translocation line YL-429 could be helpful in improving the photosynthesis of durum wheat cultivated in low-light cultivation regions. The developed markers can assist the screening of the T2DS-2AS.2AL translocation in breeding.https://www.mdpi.com/2223-7747/14/7/1077alien introgressionkaryotype analysischromosome translocationgenome resequencingglaucousness |
| spellingShingle | Sujie Yang Fan Yang Zujun Yang Wenjing Hu Hongxia Ding Feiyang Yang Hongshen Wan Zehou Liu Tao Lang Ning Yang Jie Zhang Yun Jiang Junyan Feng Hao Tang Qian Chen Qian Deng Ying Wang Jingyu Wu Jun Xiao Xing Fan Yonghong Zhou Jun Li Identification and Specific KASP Marker Development for Durum Wheat T2DS-2AS.2AL Translocation Line YL-429 with Wax Inhibitor Gene <i>IW2</i> Plants alien introgression karyotype analysis chromosome translocation genome resequencing glaucousness |
| title | Identification and Specific KASP Marker Development for Durum Wheat T2DS-2AS.2AL Translocation Line YL-429 with Wax Inhibitor Gene <i>IW2</i> |
| title_full | Identification and Specific KASP Marker Development for Durum Wheat T2DS-2AS.2AL Translocation Line YL-429 with Wax Inhibitor Gene <i>IW2</i> |
| title_fullStr | Identification and Specific KASP Marker Development for Durum Wheat T2DS-2AS.2AL Translocation Line YL-429 with Wax Inhibitor Gene <i>IW2</i> |
| title_full_unstemmed | Identification and Specific KASP Marker Development for Durum Wheat T2DS-2AS.2AL Translocation Line YL-429 with Wax Inhibitor Gene <i>IW2</i> |
| title_short | Identification and Specific KASP Marker Development for Durum Wheat T2DS-2AS.2AL Translocation Line YL-429 with Wax Inhibitor Gene <i>IW2</i> |
| title_sort | identification and specific kasp marker development for durum wheat t2ds 2as 2al translocation line yl 429 with wax inhibitor gene i iw2 i |
| topic | alien introgression karyotype analysis chromosome translocation genome resequencing glaucousness |
| url | https://www.mdpi.com/2223-7747/14/7/1077 |
| work_keys_str_mv | AT sujieyang identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT fanyang identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT zujunyang identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT wenjinghu identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT hongxiading identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT feiyangyang identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT hongshenwan identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT zehouliu identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT taolang identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT ningyang identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT jiezhang identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT yunjiang identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT junyanfeng identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT haotang identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT qianchen identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT qiandeng identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT yingwang identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT jingyuwu identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT junxiao identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT xingfan identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT yonghongzhou identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i AT junli identificationandspecifickaspmarkerdevelopmentfordurumwheatt2ds2as2altranslocationlineyl429withwaxinhibitorgeneiiw2i |