Plasma Glycosaminoglycans: A new Promising tool for Assessment of Non-Metastatic Renal Cell Carcinoma Patients Following Nephrectomy
Background: Non-invasive detection of renal cell carcinoma (RCC) recurrence is a major challenge that could radically affect patient survival. To date, there are no approved biomarkers for inclusion in the monitoring and follow-up of RCC; therefore, assessment of treatment response is lacking. Mat...
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Shahid Beheshti University of Medical Sciences
2024-09-01
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| Series: | Novelty in Biomedicine |
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| Online Access: | https://journals.sbmu.ac.ir/nbm/article/view/45088 |
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| author | Awais ALi Abdelkarem Omneya Kashif Adil |
| author_facet | Awais ALi Abdelkarem Omneya Kashif Adil |
| author_sort | Awais ALi |
| collection | DOAJ |
| description | Background: Non-invasive detection of renal cell carcinoma (RCC) recurrence is a major challenge that could radically affect patient survival. To date, there are no approved biomarkers for inclusion in the monitoring and follow-up of RCC; therefore, assessment of treatment response is lacking.
Materials and Methods: A Cross-sectional study was carried out on biopsy-proven renal cell carcinoma patients scheduled for nephrectomy at Hayatabad Medical Complex (HMC) Hospital, Peshawar, Pakistan, between September 2022 and January 2024.
Results: A total of 160 samples were included in the study. Eighty samples were obtained from biopsy-proven non-metastatic renal cell carcinoma patients, of which 40 were collected pre-nephrectomy, 40 were collected post-nephrectomy from the same patients, and 80 samples were collected from age and gender-matched healthy individuals. Total plasma glycosaminoglycans (GAGs) levels were analyzed through a manual enzyme-linked immunosorbent assay using a non-competitive sandwich technique. Quantitative variables were summarized as means and standard deviation, while qualitative variables were summarized as frequency and percentage. A paired t-test was performed to check whether the difference between the mean plasma GAG levels in pre-nephrectomy and post-nephrectomy groups was significant.
Conclusion: The results revealed that post-nephrectomy GAG levels have significantly decreased compared to pre-nephrectomy levels (P<0.001). Plasma glycosaminoglycan levels could be promising markers for monitoring renal cell carcinoma patients post-nephrectomy. |
| format | Article |
| id | doaj-art-2de2f9462c6a4bd583ca7ef98d779f48 |
| institution | Kabale University |
| issn | 2345-3907 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Shahid Beheshti University of Medical Sciences |
| record_format | Article |
| series | Novelty in Biomedicine |
| spelling | doaj-art-2de2f9462c6a4bd583ca7ef98d779f482025-01-20T05:02:54ZengShahid Beheshti University of Medical SciencesNovelty in Biomedicine2345-39072024-09-0112410.22037/nbm.v12i4.4508834977Plasma Glycosaminoglycans: A new Promising tool for Assessment of Non-Metastatic Renal Cell Carcinoma Patients Following NephrectomyAwais ALi0Abdelkarem Omneya1Kashif Adil21Department of Biochemistry, Abdul Wali Khan University Mardan, Mardan, Pakistan2Department of Chemical Pathology, Medical Research Institute, Alexandria University, Alexandria, Egypt.3Department of Urology, Hayatabad Medical Complex Hospital, PakistanBackground: Non-invasive detection of renal cell carcinoma (RCC) recurrence is a major challenge that could radically affect patient survival. To date, there are no approved biomarkers for inclusion in the monitoring and follow-up of RCC; therefore, assessment of treatment response is lacking. Materials and Methods: A Cross-sectional study was carried out on biopsy-proven renal cell carcinoma patients scheduled for nephrectomy at Hayatabad Medical Complex (HMC) Hospital, Peshawar, Pakistan, between September 2022 and January 2024. Results: A total of 160 samples were included in the study. Eighty samples were obtained from biopsy-proven non-metastatic renal cell carcinoma patients, of which 40 were collected pre-nephrectomy, 40 were collected post-nephrectomy from the same patients, and 80 samples were collected from age and gender-matched healthy individuals. Total plasma glycosaminoglycans (GAGs) levels were analyzed through a manual enzyme-linked immunosorbent assay using a non-competitive sandwich technique. Quantitative variables were summarized as means and standard deviation, while qualitative variables were summarized as frequency and percentage. A paired t-test was performed to check whether the difference between the mean plasma GAG levels in pre-nephrectomy and post-nephrectomy groups was significant. Conclusion: The results revealed that post-nephrectomy GAG levels have significantly decreased compared to pre-nephrectomy levels (P<0.001). Plasma glycosaminoglycan levels could be promising markers for monitoring renal cell carcinoma patients post-nephrectomy.https://journals.sbmu.ac.ir/nbm/article/view/45088glycosaminoglycansnephrectomyrenal cell carcinoma |
| spellingShingle | Awais ALi Abdelkarem Omneya Kashif Adil Plasma Glycosaminoglycans: A new Promising tool for Assessment of Non-Metastatic Renal Cell Carcinoma Patients Following Nephrectomy Novelty in Biomedicine glycosaminoglycans nephrectomy renal cell carcinoma |
| title | Plasma Glycosaminoglycans: A new Promising tool for Assessment of Non-Metastatic Renal Cell Carcinoma Patients Following Nephrectomy |
| title_full | Plasma Glycosaminoglycans: A new Promising tool for Assessment of Non-Metastatic Renal Cell Carcinoma Patients Following Nephrectomy |
| title_fullStr | Plasma Glycosaminoglycans: A new Promising tool for Assessment of Non-Metastatic Renal Cell Carcinoma Patients Following Nephrectomy |
| title_full_unstemmed | Plasma Glycosaminoglycans: A new Promising tool for Assessment of Non-Metastatic Renal Cell Carcinoma Patients Following Nephrectomy |
| title_short | Plasma Glycosaminoglycans: A new Promising tool for Assessment of Non-Metastatic Renal Cell Carcinoma Patients Following Nephrectomy |
| title_sort | plasma glycosaminoglycans a new promising tool for assessment of non metastatic renal cell carcinoma patients following nephrectomy |
| topic | glycosaminoglycans nephrectomy renal cell carcinoma |
| url | https://journals.sbmu.ac.ir/nbm/article/view/45088 |
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