Antiviral potential of diosmin against influenza A virus
Abstract Influenza poses a global health threat. With drug-resistant strains emerging, there is an urgent need for effective antiviral drugs. This study explores antiviral potential of flavonoids against influenza A virus (IAV) and their mechanism of action. By utilizing in silico docking as a scree...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-00744-6 |
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| author | Umarqayum AbuBakar Zhao Xuan Low Muhammad Zhafran Mohd Aris Rafidah Lani Syafiq Asnawi Zainal Abidin Muhammad-Redha Abdullah-Zawawi Pouya Hassandarvish Saiful Anuar Karsani Jasmine Elanie Khairat |
| author_facet | Umarqayum AbuBakar Zhao Xuan Low Muhammad Zhafran Mohd Aris Rafidah Lani Syafiq Asnawi Zainal Abidin Muhammad-Redha Abdullah-Zawawi Pouya Hassandarvish Saiful Anuar Karsani Jasmine Elanie Khairat |
| author_sort | Umarqayum AbuBakar |
| collection | DOAJ |
| description | Abstract Influenza poses a global health threat. With drug-resistant strains emerging, there is an urgent need for effective antiviral drugs. This study explores antiviral potential of flavonoids against influenza A virus (IAV) and their mechanism of action. By utilizing in silico docking as a screening approach, diosmin, orientin, and fisetin were identified as flavonoids with the strongest interactions with viral proteins. Out of them, diosmin was found to effectively inhibit IAV replication in vitro, particularly at the attachment and post-entry stages, with significant inhibition observed at 0-h post-infection (hpi) and 2 hpi, while also demonstrated prophylactic activity, peaking at − 2 hpi. Following that, diosmin significantly increases the expression of antiviral genes, which may relate to the discovery of its prophylactic activity. Proteomics analysis showed that diosmin treatment during the post-entry stage of IAV replication reduced viral protein levels, confirming its antiviral activity at this point. Additionally, diosmin also modulated host proteins related to innate immunity, inducing type I interferon and anti-inflammatory responses during the infection. These findings provide preliminary evidence of diosmin’s antiviral and prophylactic activity against IAV, paving the way for further research on its mechanism of action. |
| format | Article |
| id | doaj-art-2ddebb630f344eac96c90e2cec1f1721 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-2ddebb630f344eac96c90e2cec1f17212025-08-20T03:53:57ZengNature PortfolioScientific Reports2045-23222025-05-0115112610.1038/s41598-025-00744-6Antiviral potential of diosmin against influenza A virusUmarqayum AbuBakar0Zhao Xuan Low1Muhammad Zhafran Mohd Aris2Rafidah Lani3Syafiq Asnawi Zainal Abidin4Muhammad-Redha Abdullah-Zawawi5Pouya Hassandarvish6Saiful Anuar Karsani7Jasmine Elanie Khairat8Institute of Biological Sciences, Faculty of Science, Universiti MalayaTropical Infectious Diseases Research and Education Center, Universiti MalayaInstitute of Biological Sciences, Faculty of Science, Universiti MalayaDepartment of Medical Microbiology, Faculty of Medicine, Universiti MalayaJeffrey Cheah School of Medicine and Health Sciences, Monash University MalaysiaUKM Medical Molecular Biology Institute (UMBI)Tropical Infectious Diseases Research and Education Center, Universiti MalayaInstitute of Biological Sciences, Faculty of Science, Universiti MalayaInstitute of Biological Sciences, Faculty of Science, Universiti MalayaAbstract Influenza poses a global health threat. With drug-resistant strains emerging, there is an urgent need for effective antiviral drugs. This study explores antiviral potential of flavonoids against influenza A virus (IAV) and their mechanism of action. By utilizing in silico docking as a screening approach, diosmin, orientin, and fisetin were identified as flavonoids with the strongest interactions with viral proteins. Out of them, diosmin was found to effectively inhibit IAV replication in vitro, particularly at the attachment and post-entry stages, with significant inhibition observed at 0-h post-infection (hpi) and 2 hpi, while also demonstrated prophylactic activity, peaking at − 2 hpi. Following that, diosmin significantly increases the expression of antiviral genes, which may relate to the discovery of its prophylactic activity. Proteomics analysis showed that diosmin treatment during the post-entry stage of IAV replication reduced viral protein levels, confirming its antiviral activity at this point. Additionally, diosmin also modulated host proteins related to innate immunity, inducing type I interferon and anti-inflammatory responses during the infection. These findings provide preliminary evidence of diosmin’s antiviral and prophylactic activity against IAV, paving the way for further research on its mechanism of action.https://doi.org/10.1038/s41598-025-00744-6 |
| spellingShingle | Umarqayum AbuBakar Zhao Xuan Low Muhammad Zhafran Mohd Aris Rafidah Lani Syafiq Asnawi Zainal Abidin Muhammad-Redha Abdullah-Zawawi Pouya Hassandarvish Saiful Anuar Karsani Jasmine Elanie Khairat Antiviral potential of diosmin against influenza A virus Scientific Reports |
| title | Antiviral potential of diosmin against influenza A virus |
| title_full | Antiviral potential of diosmin against influenza A virus |
| title_fullStr | Antiviral potential of diosmin against influenza A virus |
| title_full_unstemmed | Antiviral potential of diosmin against influenza A virus |
| title_short | Antiviral potential of diosmin against influenza A virus |
| title_sort | antiviral potential of diosmin against influenza a virus |
| url | https://doi.org/10.1038/s41598-025-00744-6 |
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