Lugol Increases Lipolysis through Upregulation of PPAR-Gamma and Downregulation of C/EBP-Alpha in Mature 3T3-L1 Adipocytes
Overweight and obesity are defined as excessive and abnormal fat accumulation that is harmful to health. This study analyzes the effect of different concentrations of the lugol solution (molecular iodine dissolved in potassium iodide) on lipolysis in cultured 3T3-L1-differentiated adipocytes. The ma...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Journal of Nutrition and Metabolism |
| Online Access: | http://dx.doi.org/10.1155/2020/2302795 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850157578679484416 |
|---|---|
| author | Cuellar-Rufino Sergio Zepeda Rossana Citlali Flores-Muñoz Mónica Santiago-Roque Isela Arroyo-Helguera Omar |
| author_facet | Cuellar-Rufino Sergio Zepeda Rossana Citlali Flores-Muñoz Mónica Santiago-Roque Isela Arroyo-Helguera Omar |
| author_sort | Cuellar-Rufino Sergio |
| collection | DOAJ |
| description | Overweight and obesity are defined as excessive and abnormal fat accumulation that is harmful to health. This study analyzes the effect of different concentrations of the lugol solution (molecular iodine dissolved in potassium iodide) on lipolysis in cultured 3T3-L1-differentiated adipocytes. The mature adipocytes were treated with doses from 1 to 100 µm of lugol for 0.5, 6, and 24 h. The results showed that mature adipocytes exposed to lugol decrease their viability and increase caspase-3 activity with a lethal dose (LD50) of 473 µm. In mature adipocytes, lugol decreased the total intracellular lipid content, being significant at doses of 10 and 100 µm after 6 and 24 h of treatment (P<0.01), and the accumulation of intracellular triglycerides decreased after 24 h of exposure to lugol (P<0.05). Lugol treatment significantly increases the release of glycerol to the culture medium (P<0.05). The levels of adipocyte-specific transcription factors C/EBP-α were downregulated and PPAR-γ upregulated after 30 min with lugol. These results indicate a lipolytic effect of lugol dependent on PPAR-γ and C/EBP-α expression in mature 3T3-L1 adipocytes. |
| format | Article |
| id | doaj-art-2dddb17f647c4693be414725b1af5dbf |
| institution | OA Journals |
| issn | 2090-0724 2090-0732 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nutrition and Metabolism |
| spelling | doaj-art-2dddb17f647c4693be414725b1af5dbf2025-08-20T02:24:07ZengWileyJournal of Nutrition and Metabolism2090-07242090-07322020-01-01202010.1155/2020/23027952302795Lugol Increases Lipolysis through Upregulation of PPAR-Gamma and Downregulation of C/EBP-Alpha in Mature 3T3-L1 AdipocytesCuellar-Rufino Sergio0Zepeda Rossana Citlali1Flores-Muñoz Mónica2Santiago-Roque Isela3Arroyo-Helguera Omar4Centro de Investigaciones Biomédicas, Universidad Veracruzana, Av. Luis Castelazo Ayala S/N, Col. Industrial Ánimas, Xalapa 91190, Veracruz, MexicoCentro de Investigaciones Biomédicas, Universidad Veracruzana, Av. Luis Castelazo Ayala S/N, Col. Industrial Ánimas, Xalapa 91190, Veracruz, MexicoInstituto de Ciencias de la Salud, Universidad Veracruzana, Av. Luis Castelazo Ayala S/N, Col. Industrial Ánimas, Xalapa 91190, Veracruz, MexicoFacultad de Bioanálisis, Universidad Veracruzana, Odontólogos W/N, U. H. del Bosque, Xalapa 91190, Veracruz, MexicoBiomedicine in Public Health Laboratory, Public Health Institute, Universidad Veracruzana, Av. Luís Castelazo Ayala S/N, Col. Industrial Animas, Xalapa 91190, Veracruz, MexicoOverweight and obesity are defined as excessive and abnormal fat accumulation that is harmful to health. This study analyzes the effect of different concentrations of the lugol solution (molecular iodine dissolved in potassium iodide) on lipolysis in cultured 3T3-L1-differentiated adipocytes. The mature adipocytes were treated with doses from 1 to 100 µm of lugol for 0.5, 6, and 24 h. The results showed that mature adipocytes exposed to lugol decrease their viability and increase caspase-3 activity with a lethal dose (LD50) of 473 µm. In mature adipocytes, lugol decreased the total intracellular lipid content, being significant at doses of 10 and 100 µm after 6 and 24 h of treatment (P<0.01), and the accumulation of intracellular triglycerides decreased after 24 h of exposure to lugol (P<0.05). Lugol treatment significantly increases the release of glycerol to the culture medium (P<0.05). The levels of adipocyte-specific transcription factors C/EBP-α were downregulated and PPAR-γ upregulated after 30 min with lugol. These results indicate a lipolytic effect of lugol dependent on PPAR-γ and C/EBP-α expression in mature 3T3-L1 adipocytes.http://dx.doi.org/10.1155/2020/2302795 |
| spellingShingle | Cuellar-Rufino Sergio Zepeda Rossana Citlali Flores-Muñoz Mónica Santiago-Roque Isela Arroyo-Helguera Omar Lugol Increases Lipolysis through Upregulation of PPAR-Gamma and Downregulation of C/EBP-Alpha in Mature 3T3-L1 Adipocytes Journal of Nutrition and Metabolism |
| title | Lugol Increases Lipolysis through Upregulation of PPAR-Gamma and Downregulation of C/EBP-Alpha in Mature 3T3-L1 Adipocytes |
| title_full | Lugol Increases Lipolysis through Upregulation of PPAR-Gamma and Downregulation of C/EBP-Alpha in Mature 3T3-L1 Adipocytes |
| title_fullStr | Lugol Increases Lipolysis through Upregulation of PPAR-Gamma and Downregulation of C/EBP-Alpha in Mature 3T3-L1 Adipocytes |
| title_full_unstemmed | Lugol Increases Lipolysis through Upregulation of PPAR-Gamma and Downregulation of C/EBP-Alpha in Mature 3T3-L1 Adipocytes |
| title_short | Lugol Increases Lipolysis through Upregulation of PPAR-Gamma and Downregulation of C/EBP-Alpha in Mature 3T3-L1 Adipocytes |
| title_sort | lugol increases lipolysis through upregulation of ppar gamma and downregulation of c ebp alpha in mature 3t3 l1 adipocytes |
| url | http://dx.doi.org/10.1155/2020/2302795 |
| work_keys_str_mv | AT cuellarrufinosergio lugolincreaseslipolysisthroughupregulationofppargammaanddownregulationofcebpalphainmature3t3l1adipocytes AT zepedarossanacitlali lugolincreaseslipolysisthroughupregulationofppargammaanddownregulationofcebpalphainmature3t3l1adipocytes AT floresmunozmonica lugolincreaseslipolysisthroughupregulationofppargammaanddownregulationofcebpalphainmature3t3l1adipocytes AT santiagoroqueisela lugolincreaseslipolysisthroughupregulationofppargammaanddownregulationofcebpalphainmature3t3l1adipocytes AT arroyohelgueraomar lugolincreaseslipolysisthroughupregulationofppargammaanddownregulationofcebpalphainmature3t3l1adipocytes |