Biothermodynamic Analysis of Norovirus: Mechanistic Model of Virus–Host Interactions and Virus–Virus Competition Based on Gibbs Energy

Norovirus is a leading cause of viral gastroenteritis worldwide and has been studied extensively from the perspective of life and biomedical sciences. However, no biothermodynamic analysis of Norovirus has been reported in the literature. Such an analysis would provide insights into the role of ener...

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Main Authors: Marko E. Popović, Vojin Tadić, Marijana Pantović Pavlović
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Microbiology Research
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Online Access:https://www.mdpi.com/2036-7481/16/6/112
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author Marko E. Popović
Vojin Tadić
Marijana Pantović Pavlović
author_facet Marko E. Popović
Vojin Tadić
Marijana Pantović Pavlović
author_sort Marko E. Popović
collection DOAJ
description Norovirus is a leading cause of viral gastroenteritis worldwide and has been studied extensively from the perspective of life and biomedical sciences. However, no biothermodynamic analysis of Norovirus has been reported in the literature. Such an analysis would provide insights into the role of energetic constraints in the interactions between Norovirus and its host cells and other viruses. In this research, Norovirus was characterized from the aspect of chemistry and chemical thermodynamics, with the determination of its molecular formula, empirical formula, molar mass and thermodynamic properties (enthalpy, entropy, Gibbs energy) of formation. Based on these properties, biosynthesis reactions were formulated that show how Norovirus particles are synthetized inside host cells, and the thermodynamic properties of biosynthesis were determined. Moreover, the thermodynamic properties of the binding of Norovirus to its host cell receptor were determined. These were then used to develop a model of virus–host interactions at the cell membrane (antigen-receptor binding) and inside the cytoplasm (virus multiplication), with the phenomenological equations of nonequilibrium thermodynamics. Based on the model, an analysis of the virus–virus competition between Norovirus and Rotavirus was conducted.
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spelling doaj-art-2dd9ddd3bbf441b88decaa2471f246bc2025-08-20T03:27:21ZengMDPI AGMicrobiology Research2036-74812025-06-0116611210.3390/microbiolres16060112Biothermodynamic Analysis of Norovirus: Mechanistic Model of Virus–Host Interactions and Virus–Virus Competition Based on Gibbs EnergyMarko E. Popović0Vojin Tadić1Marijana Pantović Pavlović2Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoševa 12, 11000 Belgrade, SerbiaDepartment for Experimental Testing of Precious Metals, Mining and Metallurgy Institute, Zeleni Bulevar 35, 19210 Bor, SerbiaInstitute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoševa 12, 11000 Belgrade, SerbiaNorovirus is a leading cause of viral gastroenteritis worldwide and has been studied extensively from the perspective of life and biomedical sciences. However, no biothermodynamic analysis of Norovirus has been reported in the literature. Such an analysis would provide insights into the role of energetic constraints in the interactions between Norovirus and its host cells and other viruses. In this research, Norovirus was characterized from the aspect of chemistry and chemical thermodynamics, with the determination of its molecular formula, empirical formula, molar mass and thermodynamic properties (enthalpy, entropy, Gibbs energy) of formation. Based on these properties, biosynthesis reactions were formulated that show how Norovirus particles are synthetized inside host cells, and the thermodynamic properties of biosynthesis were determined. Moreover, the thermodynamic properties of the binding of Norovirus to its host cell receptor were determined. These were then used to develop a model of virus–host interactions at the cell membrane (antigen-receptor binding) and inside the cytoplasm (virus multiplication), with the phenomenological equations of nonequilibrium thermodynamics. Based on the model, an analysis of the virus–virus competition between Norovirus and Rotavirus was conducted.https://www.mdpi.com/2036-7481/16/6/112antigen-receptor bindingvirus multiplicationinfectivitypathogenicityepidemicdriving force
spellingShingle Marko E. Popović
Vojin Tadić
Marijana Pantović Pavlović
Biothermodynamic Analysis of Norovirus: Mechanistic Model of Virus–Host Interactions and Virus–Virus Competition Based on Gibbs Energy
Microbiology Research
antigen-receptor binding
virus multiplication
infectivity
pathogenicity
epidemic
driving force
title Biothermodynamic Analysis of Norovirus: Mechanistic Model of Virus–Host Interactions and Virus–Virus Competition Based on Gibbs Energy
title_full Biothermodynamic Analysis of Norovirus: Mechanistic Model of Virus–Host Interactions and Virus–Virus Competition Based on Gibbs Energy
title_fullStr Biothermodynamic Analysis of Norovirus: Mechanistic Model of Virus–Host Interactions and Virus–Virus Competition Based on Gibbs Energy
title_full_unstemmed Biothermodynamic Analysis of Norovirus: Mechanistic Model of Virus–Host Interactions and Virus–Virus Competition Based on Gibbs Energy
title_short Biothermodynamic Analysis of Norovirus: Mechanistic Model of Virus–Host Interactions and Virus–Virus Competition Based on Gibbs Energy
title_sort biothermodynamic analysis of norovirus mechanistic model of virus host interactions and virus virus competition based on gibbs energy
topic antigen-receptor binding
virus multiplication
infectivity
pathogenicity
epidemic
driving force
url https://www.mdpi.com/2036-7481/16/6/112
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AT marijanapantovicpavlovic biothermodynamicanalysisofnorovirusmechanisticmodelofvirushostinteractionsandvirusviruscompetitionbasedongibbsenergy