Nanofibrous dressings incorporating a synergistic antibacterial-anti-inflammatory effect for infected wound healing
Bacterial infections and chronic inflammation disrupt wound immune homeostasis and impair healing progression. Herein, we report a novel nanofibrous dressings that exhibits a synergistic antibacterial-anti-inflammatory effect through the integration of the physical barrier properties of electrospun...
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Elsevier
2025-10-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425007252 |
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| author | Wei Xu Tingting Xu Lei Yu Xuchao Ning Chunling Zhang Bingcheng Yi Wufei Dai Zhihua Zhu Haiguang Zhao |
| author_facet | Wei Xu Tingting Xu Lei Yu Xuchao Ning Chunling Zhang Bingcheng Yi Wufei Dai Zhihua Zhu Haiguang Zhao |
| author_sort | Wei Xu |
| collection | DOAJ |
| description | Bacterial infections and chronic inflammation disrupt wound immune homeostasis and impair healing progression. Herein, we report a novel nanofibrous dressings that exhibits a synergistic antibacterial-anti-inflammatory effect through the integration of the physical barrier properties of electrospun nanofibers, the antimicrobial activity of biomacromolecule polylysine (PLys), and the anti-inflammatory and antioxidant effects of natural macromolecule tannic acid (TA). Using poly(L-lactide-co-ε-caprolactone) (PLCL) as the base biomaterial, sequential surface modification with TA and PLys enhanced wettability and introduced a positive surface charge, yielding a dressing with exceptional cytocompatibility and potent antimicrobial activity against Staphylococcus aureus. In vitro studies revealed that the PLys-grafted, mussel-inspired nanofibers (PLys@TA@PLCL) modulated the wound microenvironment by suppressing fibroblast-to-myofibroblast differentiation, mitigating pro-inflammatory responses, and accelerating extracellular matrix (ECM) deposition and remodeling. In vivo evaluations demonstrated that PLys@TA@PLCL nanofibers effectively reduced bacterial burden and prevented yellow scab formation, while accelerating healing of infected wounds. Notably, these dressings exhibited robust anti-inflammatory activity during early stages of repair and promoted near-complete wound closure by 2 weeks, accompanied by enhanced collagen synthesis, skin regeneration, and hair follicle neogenesis. Collectively, these findings highlight the therapeutic potential of PLys@TA-modified nanofibrous dressings for managing chronic, full-thickness wounds. |
| format | Article |
| id | doaj-art-2dd4f0cb84da4caf8d8270e7646ea2f6 |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-10-01 |
| publisher | Elsevier |
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| series | Materials Today Bio |
| spelling | doaj-art-2dd4f0cb84da4caf8d8270e7646ea2f62025-08-20T05:07:28ZengElsevierMaterials Today Bio2590-00642025-10-013410215510.1016/j.mtbio.2025.102155Nanofibrous dressings incorporating a synergistic antibacterial-anti-inflammatory effect for infected wound healingWei Xu0Tingting Xu1Lei Yu2Xuchao Ning3Chunling Zhang4Bingcheng Yi5Wufei Dai6Zhihua Zhu7Haiguang Zhao8Department of Plastic Surgery, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, Qingdao, Shandong, ChinaDepartment of Traditional Chinese Medicine, Chengyang District People's Hospital, Qingdao, Shandong, China; College of Biological Science and Medical Engineering, Donghua University, Shanghai, ChinaDepartment of Traditional Chinese Medicine, Qingdao Central Hospital, University of Health and Rehabilitation Sciences (Qingdao Central Hospital), Qingdao, Shandong, ChinaDepartment of Plastic Surgery, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, Qingdao, Shandong, ChinaDepartment of Traditional Chinese Medicine, Qingdao Central Hospital, University of Health and Rehabilitation Sciences (Qingdao Central Hospital), Qingdao, Shandong, ChinaDepartment of Traditional Chinese Medicine, Qingdao Central Hospital, University of Health and Rehabilitation Sciences (Qingdao Central Hospital), Qingdao, Shandong, China; Qingdao Key Laboratory of Materials for Tissue Repair and Rehabilitation, Shandong Engineering Research Center for Tissue Rehabilitation Materials and Devices, School of Rehabilitation Sciences and Engineering, University of Health and Rehabilitation Sciences, Qingdao, Shandong, China; Corresponding author. Qingdao Key Laboratory of Materials for Tissue Repair and Rehabilitation, Shandong Engineering Research Center for Tissue Rehabilitation Materials and Devices, School of Rehabilitation Sciences and Engineering, University of Health and Rehabilitation Sciences, Qingdao, Shandong, China.Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of medicine 1, Universitätklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany; Corresponding author. Department of medicine 1, Universitätklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany.Department of Traditional Chinese Medicine, Chengyang District People's Hospital, Qingdao, Shandong, China; Corresponding author. Department of Traditional Chinese Medicine, Chengyang District People's Hospital, Qingdao, Shandong, China.Department of Plastic Surgery, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, Qingdao, Shandong, China; Corresponding author. Department of Plastic Surgery, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, Qingdao, Shandong, China.Bacterial infections and chronic inflammation disrupt wound immune homeostasis and impair healing progression. Herein, we report a novel nanofibrous dressings that exhibits a synergistic antibacterial-anti-inflammatory effect through the integration of the physical barrier properties of electrospun nanofibers, the antimicrobial activity of biomacromolecule polylysine (PLys), and the anti-inflammatory and antioxidant effects of natural macromolecule tannic acid (TA). Using poly(L-lactide-co-ε-caprolactone) (PLCL) as the base biomaterial, sequential surface modification with TA and PLys enhanced wettability and introduced a positive surface charge, yielding a dressing with exceptional cytocompatibility and potent antimicrobial activity against Staphylococcus aureus. In vitro studies revealed that the PLys-grafted, mussel-inspired nanofibers (PLys@TA@PLCL) modulated the wound microenvironment by suppressing fibroblast-to-myofibroblast differentiation, mitigating pro-inflammatory responses, and accelerating extracellular matrix (ECM) deposition and remodeling. In vivo evaluations demonstrated that PLys@TA@PLCL nanofibers effectively reduced bacterial burden and prevented yellow scab formation, while accelerating healing of infected wounds. Notably, these dressings exhibited robust anti-inflammatory activity during early stages of repair and promoted near-complete wound closure by 2 weeks, accompanied by enhanced collagen synthesis, skin regeneration, and hair follicle neogenesis. Collectively, these findings highlight the therapeutic potential of PLys@TA-modified nanofibrous dressings for managing chronic, full-thickness wounds.http://www.sciencedirect.com/science/article/pii/S2590006425007252Bacterial infectionHyperinflammatory responseTannic acidPolylysineChronic wound |
| spellingShingle | Wei Xu Tingting Xu Lei Yu Xuchao Ning Chunling Zhang Bingcheng Yi Wufei Dai Zhihua Zhu Haiguang Zhao Nanofibrous dressings incorporating a synergistic antibacterial-anti-inflammatory effect for infected wound healing Materials Today Bio Bacterial infection Hyperinflammatory response Tannic acid Polylysine Chronic wound |
| title | Nanofibrous dressings incorporating a synergistic antibacterial-anti-inflammatory effect for infected wound healing |
| title_full | Nanofibrous dressings incorporating a synergistic antibacterial-anti-inflammatory effect for infected wound healing |
| title_fullStr | Nanofibrous dressings incorporating a synergistic antibacterial-anti-inflammatory effect for infected wound healing |
| title_full_unstemmed | Nanofibrous dressings incorporating a synergistic antibacterial-anti-inflammatory effect for infected wound healing |
| title_short | Nanofibrous dressings incorporating a synergistic antibacterial-anti-inflammatory effect for infected wound healing |
| title_sort | nanofibrous dressings incorporating a synergistic antibacterial anti inflammatory effect for infected wound healing |
| topic | Bacterial infection Hyperinflammatory response Tannic acid Polylysine Chronic wound |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425007252 |
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