In Vitro Response of Two Strains of <i>Cordyceps javanica</i> to Six Chemical Pesticides

To determine the compatibility of two new biocontrol fungi with common chemical pesticides, this study examined the effects of three insecticides, namely, avermectin, imidacloprid, and acetamiprid, and three fungicides, namely, chlorogenonil, boscalid, and kasugamycin, on the mycelial growth and spo...

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Main Authors: Ruixia Mao, Xiaoxia Cai, Tengyu Wang, Ziyang Liu, Peixiang Xing, Guisen Zhang, Wenwen Zhou, Hongliang Diao, Ruiyan Ma
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Journal of Fungi
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Online Access:https://www.mdpi.com/2309-608X/10/12/852
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author Ruixia Mao
Xiaoxia Cai
Tengyu Wang
Ziyang Liu
Peixiang Xing
Guisen Zhang
Wenwen Zhou
Hongliang Diao
Ruiyan Ma
author_facet Ruixia Mao
Xiaoxia Cai
Tengyu Wang
Ziyang Liu
Peixiang Xing
Guisen Zhang
Wenwen Zhou
Hongliang Diao
Ruiyan Ma
author_sort Ruixia Mao
collection DOAJ
description To determine the compatibility of two new biocontrol fungi with common chemical pesticides, this study examined the effects of three insecticides, namely, avermectin, imidacloprid, and acetamiprid, and three fungicides, namely, chlorogenonil, boscalid, and kasugamycin, on the mycelial growth and spore germination of <i>Cordyceps javanica</i> strains IF-1106 and IJ-tg19. The insecticidal effects of mixed insecticides or fungicides with good compatibility with <i>C. javanica</i> IJ-tg19 against <i>Myzus persicae</i> were tested. The results showed that the six chemical pesticides exerted different degrees of inhibition on the mycelial growth of both <i>C. javanica</i> strains, with an obvious dose-dependent effect. The inhibitory effect of chlorothalonil on the mycelial growth of IF-1106 and IJ-tg19 was greater than 75%. Different kinds and concentrations of chemical pesticides had significant effects on spore germination. Among them, acetamiprid had little inhibitory effect on <i>C. javanica</i> spores. Therefore, the two <i>C. javanica</i> strains exhibited good compatibility with the insecticide acetamiprid and had some compatibility with avermectin and imidacloprid. Among the fungicides, the compatibility of the two strains of biocontrol fungi was the best with kasugamycin, followed by boscalid, while their compatibility with chlorothalonil showed the least compatibility. The median lethal time (LT<sub>50</sub>) of five concentrations of <i>C. javanica</i> IJ-tg19 (1 × 10<sup>3</sup>, 1 × 10<sup>4</sup>, 1 × 10<sup>5</sup>, 1 × 10<sup>6</sup>, and 1 × 10<sup>7</sup> spore/mL) mixed with acetamiprid against <i>M. persicae</i> were 5.28, 4.56, 3.80, 2.73, and 2.13 days, respectively, and the insecticidal rate was higher than that of fungus treatment alone (5.19, 4.59, 4.05, 3.32, and 2.94 days, respectively) or chemical pesticide treatment (5.36 days). This study provides data support and a theoretical basis for reducing the use of chemical pesticides, improving the efficiency of <i>C. javanica</i>-based insecticides, and optimizing the synergistic use of fungi and chemical pesticides.
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spelling doaj-art-2dd216b76aa84e26b28b0f2661ee87fe2025-08-20T02:53:41ZengMDPI AGJournal of Fungi2309-608X2024-12-01101285210.3390/jof10120852In Vitro Response of Two Strains of <i>Cordyceps javanica</i> to Six Chemical PesticidesRuixia Mao0Xiaoxia Cai1Tengyu Wang2Ziyang Liu3Peixiang Xing4Guisen Zhang5Wenwen Zhou6Hongliang Diao7Ruiyan Ma8College of Plant Protection, Shanxi Agricultural University, Taiyuan 030031, ChinaCollege of Plant Protection, Shanxi Agricultural University, Taiyuan 030031, ChinaCollege of Plant Protection, Shanxi Agricultural University, Taiyuan 030031, ChinaCollege of Plant Protection, Shanxi Agricultural University, Taiyuan 030031, ChinaCollege of Plant Protection, Shanxi Agricultural University, Taiyuan 030031, ChinaCollege of Plant Protection, Shanxi Agricultural University, Taiyuan 030031, ChinaCollege of Plant Protection, Shanxi Agricultural University, Taiyuan 030031, ChinaCollege of Plant Protection, Shanxi Agricultural University, Taiyuan 030031, ChinaCollege of Plant Protection, Shanxi Agricultural University, Taiyuan 030031, ChinaTo determine the compatibility of two new biocontrol fungi with common chemical pesticides, this study examined the effects of three insecticides, namely, avermectin, imidacloprid, and acetamiprid, and three fungicides, namely, chlorogenonil, boscalid, and kasugamycin, on the mycelial growth and spore germination of <i>Cordyceps javanica</i> strains IF-1106 and IJ-tg19. The insecticidal effects of mixed insecticides or fungicides with good compatibility with <i>C. javanica</i> IJ-tg19 against <i>Myzus persicae</i> were tested. The results showed that the six chemical pesticides exerted different degrees of inhibition on the mycelial growth of both <i>C. javanica</i> strains, with an obvious dose-dependent effect. The inhibitory effect of chlorothalonil on the mycelial growth of IF-1106 and IJ-tg19 was greater than 75%. Different kinds and concentrations of chemical pesticides had significant effects on spore germination. Among them, acetamiprid had little inhibitory effect on <i>C. javanica</i> spores. Therefore, the two <i>C. javanica</i> strains exhibited good compatibility with the insecticide acetamiprid and had some compatibility with avermectin and imidacloprid. Among the fungicides, the compatibility of the two strains of biocontrol fungi was the best with kasugamycin, followed by boscalid, while their compatibility with chlorothalonil showed the least compatibility. The median lethal time (LT<sub>50</sub>) of five concentrations of <i>C. javanica</i> IJ-tg19 (1 × 10<sup>3</sup>, 1 × 10<sup>4</sup>, 1 × 10<sup>5</sup>, 1 × 10<sup>6</sup>, and 1 × 10<sup>7</sup> spore/mL) mixed with acetamiprid against <i>M. persicae</i> were 5.28, 4.56, 3.80, 2.73, and 2.13 days, respectively, and the insecticidal rate was higher than that of fungus treatment alone (5.19, 4.59, 4.05, 3.32, and 2.94 days, respectively) or chemical pesticide treatment (5.36 days). This study provides data support and a theoretical basis for reducing the use of chemical pesticides, improving the efficiency of <i>C. javanica</i>-based insecticides, and optimizing the synergistic use of fungi and chemical pesticides.https://www.mdpi.com/2309-608X/10/12/852chemical pesticides<i>Cordyceps javanica</i>compatibilitygrowth inhibitionbiological control
spellingShingle Ruixia Mao
Xiaoxia Cai
Tengyu Wang
Ziyang Liu
Peixiang Xing
Guisen Zhang
Wenwen Zhou
Hongliang Diao
Ruiyan Ma
In Vitro Response of Two Strains of <i>Cordyceps javanica</i> to Six Chemical Pesticides
Journal of Fungi
chemical pesticides
<i>Cordyceps javanica</i>
compatibility
growth inhibition
biological control
title In Vitro Response of Two Strains of <i>Cordyceps javanica</i> to Six Chemical Pesticides
title_full In Vitro Response of Two Strains of <i>Cordyceps javanica</i> to Six Chemical Pesticides
title_fullStr In Vitro Response of Two Strains of <i>Cordyceps javanica</i> to Six Chemical Pesticides
title_full_unstemmed In Vitro Response of Two Strains of <i>Cordyceps javanica</i> to Six Chemical Pesticides
title_short In Vitro Response of Two Strains of <i>Cordyceps javanica</i> to Six Chemical Pesticides
title_sort in vitro response of two strains of i cordyceps javanica i to six chemical pesticides
topic chemical pesticides
<i>Cordyceps javanica</i>
compatibility
growth inhibition
biological control
url https://www.mdpi.com/2309-608X/10/12/852
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