Effects of Liquid Bio-Fertilizer on Plant Growth, Antioxidant Activity, and Soil Bacterial Community During Cultivation of Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>)

This study investigated the effects of liquid bio-fertilizer (LBF) on the growth, antioxidant activity, soil properties, and soil microbial composition of Chinese cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>). The LBF application significantly enhanced vegetative g...

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Main Authors: Tran Yen Linh Le, Junkyung Lee, Su-Yeon Shim, Jiwon Jung, Soo-Ryang Kim, Sung-Ha Hong, Myung-Gyu Lee, Sun-Goo Hwang
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/13/5/1036
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author Tran Yen Linh Le
Junkyung Lee
Su-Yeon Shim
Jiwon Jung
Soo-Ryang Kim
Sung-Ha Hong
Myung-Gyu Lee
Sun-Goo Hwang
author_facet Tran Yen Linh Le
Junkyung Lee
Su-Yeon Shim
Jiwon Jung
Soo-Ryang Kim
Sung-Ha Hong
Myung-Gyu Lee
Sun-Goo Hwang
author_sort Tran Yen Linh Le
collection DOAJ
description This study investigated the effects of liquid bio-fertilizer (LBF) on the growth, antioxidant activity, soil properties, and soil microbial composition of Chinese cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>). The LBF application significantly enhanced vegetative growth by increasing the leaf length, leaf width, fresh weight, and dry weight. Additionally, antioxidant activity increased with rises in total phenolic and flavonoid contents. However, the per-unit antioxidant concentrations decreased, likely due to rapid biomass accumulation. Soil analysis showed improvements in pH, organic matter, and available phosphorus. Microbial analysis revealed that <i>Acidobacteria</i> enrichment was associated with enhanced nutrient cycling despite reduced overall microbial diversity. Transcriptomic analysis identified 445 differentially expressed genes with upregulation in the metabolism and photosynthesis-related pathways, suggesting improved nutrient assimilation and energy production. These findings demonstrate that LBF enhances plant growth and soil fertility while influencing microbial dynamics and gene expression.
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publisher MDPI AG
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series Microorganisms
spelling doaj-art-2dceee2d965b4b6499f96fa8e95451fc2025-08-20T02:33:51ZengMDPI AGMicroorganisms2076-26072025-04-01135103610.3390/microorganisms13051036Effects of Liquid Bio-Fertilizer on Plant Growth, Antioxidant Activity, and Soil Bacterial Community During Cultivation of Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>)Tran Yen Linh Le0Junkyung Lee1Su-Yeon Shim2Jiwon Jung3Soo-Ryang Kim4Sung-Ha Hong5Myung-Gyu Lee6Sun-Goo Hwang7Department of Agricultural Convergence, Sangji University, 83 Sangjidae-gil, Wonju-si 26339, Republic of KoreaDepartment of Applied Plant Science, Sangji University, 83 Sangjidae-gil, Wonju-si 26339, Republic of KoreaDepartment of Applied Plant Science, Sangji University, 83 Sangjidae-gil, Wonju-si 26339, Republic of KoreaDepartment of Environmental Resources, Agricultural and Rural Development, Sangji University, 83 Sangjidae-gil, Wonju-si 26339, Republic of KoreaIndustry-Academic Cooperation Foundation, Sangji University, 83 Sangjidae-gil, Wonju-si 26339, Republic of KoreaIndustry-Academic Cooperation Foundation, Sangji University, 83 Sangjidae-gil, Wonju-si 26339, Republic of KoreaDepartment of Smart Life Science, Sangji University, 83 Sangjidae-gil, Wonju-si 26339, Republic of KoreaDepartment of Smart Life Science, Sangji University, 83 Sangjidae-gil, Wonju-si 26339, Republic of KoreaThis study investigated the effects of liquid bio-fertilizer (LBF) on the growth, antioxidant activity, soil properties, and soil microbial composition of Chinese cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>). The LBF application significantly enhanced vegetative growth by increasing the leaf length, leaf width, fresh weight, and dry weight. Additionally, antioxidant activity increased with rises in total phenolic and flavonoid contents. However, the per-unit antioxidant concentrations decreased, likely due to rapid biomass accumulation. Soil analysis showed improvements in pH, organic matter, and available phosphorus. Microbial analysis revealed that <i>Acidobacteria</i> enrichment was associated with enhanced nutrient cycling despite reduced overall microbial diversity. Transcriptomic analysis identified 445 differentially expressed genes with upregulation in the metabolism and photosynthesis-related pathways, suggesting improved nutrient assimilation and energy production. These findings demonstrate that LBF enhances plant growth and soil fertility while influencing microbial dynamics and gene expression.https://www.mdpi.com/2076-2607/13/5/1036liquid bio-fertilizerplant growthantioxidantsoil nutrientssoil microbial diversity
spellingShingle Tran Yen Linh Le
Junkyung Lee
Su-Yeon Shim
Jiwon Jung
Soo-Ryang Kim
Sung-Ha Hong
Myung-Gyu Lee
Sun-Goo Hwang
Effects of Liquid Bio-Fertilizer on Plant Growth, Antioxidant Activity, and Soil Bacterial Community During Cultivation of Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>)
Microorganisms
liquid bio-fertilizer
plant growth
antioxidant
soil nutrients
soil microbial diversity
title Effects of Liquid Bio-Fertilizer on Plant Growth, Antioxidant Activity, and Soil Bacterial Community During Cultivation of Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>)
title_full Effects of Liquid Bio-Fertilizer on Plant Growth, Antioxidant Activity, and Soil Bacterial Community During Cultivation of Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>)
title_fullStr Effects of Liquid Bio-Fertilizer on Plant Growth, Antioxidant Activity, and Soil Bacterial Community During Cultivation of Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>)
title_full_unstemmed Effects of Liquid Bio-Fertilizer on Plant Growth, Antioxidant Activity, and Soil Bacterial Community During Cultivation of Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>)
title_short Effects of Liquid Bio-Fertilizer on Plant Growth, Antioxidant Activity, and Soil Bacterial Community During Cultivation of Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>)
title_sort effects of liquid bio fertilizer on plant growth antioxidant activity and soil bacterial community during cultivation of chinese cabbage i brassica rapa i l ssp i pekinensis i
topic liquid bio-fertilizer
plant growth
antioxidant
soil nutrients
soil microbial diversity
url https://www.mdpi.com/2076-2607/13/5/1036
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