Production of Conidia by Entomopathogenic Fungi and Their Pathogenicity Against Coptotermes sp.
Entomopathogenic fungi have the potential to infect most arthropods including termites which are economically important major insects pest of wood, wood products and building structures. However, the application of this fungus in the field has not shown satisfactory results yet, one of which is cons...
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Universitas Negeri Semarang
2020-04-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
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| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/22435 |
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| author | Deni Zulfiana Apriwi Zulfitri Anis Sri Lestari Ni Putu Ratna Ayu Krishanti Dita Meisyara |
| author_facet | Deni Zulfiana Apriwi Zulfitri Anis Sri Lestari Ni Putu Ratna Ayu Krishanti Dita Meisyara |
| author_sort | Deni Zulfiana |
| collection | DOAJ |
| description | Entomopathogenic fungi have the potential to infect most arthropods including termites which are economically important major insects pest of wood, wood products and building structures. However, the application of this fungus in the field has not shown satisfactory results yet, one of which is constrained in mass production of conidia. The purpose of this study was to evaluate 16 types of biodegradable products and waste as substrates for mass production of conidia using solid state fermentation method and two types of inoculum namely solid and liquid inoculum. Toxicity tests were carried out on subterranean termites (Coptotermes sp.) based on JIS K 1571, 2010. The parameters observed were the number and dry weight of the conidia produced, conidial viability, nutrient content of the substrate, and percentage of termite mortality. The results showed that rice, sorghum and corn were the best media for the growth of entomopathogenic fungi based on the number of conidia and dry weight of the conidia produced. Metarhizium sp. T4.B23 produced the highest number of conidia, 1.12 x 1011 conidia/100 g substrate and yielded 180.9 ± 0.623 g dry conidia/kg of rice; followed by Metarhizium sp. B2.2 grown on sorghum that resulted in 1.11 x 1010 conidia/100 g substrates and 8 ± 0.570 g /kg sorghum; and B. bassiana produced 8.3 x 109 conidia/100 g substrate and 31.24 ± 0.407 gr/ kg sorghum. Metarhizium sp. B2.2 showed the highest toxicity to termites with 100% mortality was observed within the second day of testing. Therefore the conidia of Metahizium sp. B2.2 is potential to be developed as a biopesticide using rice or sorghum substrate as a carrier. |
| format | Article |
| id | doaj-art-2dc5e18e2ff541afac97285a52bfbec3 |
| institution | DOAJ |
| issn | 2085-191X 2338-7610 |
| language | English |
| publishDate | 2020-04-01 |
| publisher | Universitas Negeri Semarang |
| record_format | Article |
| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj-art-2dc5e18e2ff541afac97285a52bfbec32025-08-20T03:20:26ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102020-04-011211910.15294/biosaintifika.v12i1.224359575Production of Conidia by Entomopathogenic Fungi and Their Pathogenicity Against Coptotermes sp.Deni Zulfiana0Apriwi Zulfitri1Anis Sri Lestari2Ni Putu Ratna Ayu Krishanti3Dita Meisyara4Research Center for Biomaterials, Indonesian Institute of Sciences-LIPIResearch Center for Biomaterials, Indonesian Institute of Sciences-LIPIResearch Center for Biomaterials, Indonesian Institute of Sciences-LIPIResearch Center for Biomaterials, Indonesian Institute of Sciences-LIPIResearch Center for Biomaterials, Indonesian Institute of Sciences-LIPIEntomopathogenic fungi have the potential to infect most arthropods including termites which are economically important major insects pest of wood, wood products and building structures. However, the application of this fungus in the field has not shown satisfactory results yet, one of which is constrained in mass production of conidia. The purpose of this study was to evaluate 16 types of biodegradable products and waste as substrates for mass production of conidia using solid state fermentation method and two types of inoculum namely solid and liquid inoculum. Toxicity tests were carried out on subterranean termites (Coptotermes sp.) based on JIS K 1571, 2010. The parameters observed were the number and dry weight of the conidia produced, conidial viability, nutrient content of the substrate, and percentage of termite mortality. The results showed that rice, sorghum and corn were the best media for the growth of entomopathogenic fungi based on the number of conidia and dry weight of the conidia produced. Metarhizium sp. T4.B23 produced the highest number of conidia, 1.12 x 1011 conidia/100 g substrate and yielded 180.9 ± 0.623 g dry conidia/kg of rice; followed by Metarhizium sp. B2.2 grown on sorghum that resulted in 1.11 x 1010 conidia/100 g substrates and 8 ± 0.570 g /kg sorghum; and B. bassiana produced 8.3 x 109 conidia/100 g substrate and 31.24 ± 0.407 gr/ kg sorghum. Metarhizium sp. B2.2 showed the highest toxicity to termites with 100% mortality was observed within the second day of testing. Therefore the conidia of Metahizium sp. B2.2 is potential to be developed as a biopesticide using rice or sorghum substrate as a carrier.https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/22435beauveria, biopesticide, coptotermes, entomopathogenic fungi, metarhizium |
| spellingShingle | Deni Zulfiana Apriwi Zulfitri Anis Sri Lestari Ni Putu Ratna Ayu Krishanti Dita Meisyara Production of Conidia by Entomopathogenic Fungi and Their Pathogenicity Against Coptotermes sp. Biosaintifika: Journal of Biology & Biology Education beauveria, biopesticide, coptotermes, entomopathogenic fungi, metarhizium |
| title | Production of Conidia by Entomopathogenic Fungi and Their Pathogenicity Against Coptotermes sp. |
| title_full | Production of Conidia by Entomopathogenic Fungi and Their Pathogenicity Against Coptotermes sp. |
| title_fullStr | Production of Conidia by Entomopathogenic Fungi and Their Pathogenicity Against Coptotermes sp. |
| title_full_unstemmed | Production of Conidia by Entomopathogenic Fungi and Their Pathogenicity Against Coptotermes sp. |
| title_short | Production of Conidia by Entomopathogenic Fungi and Their Pathogenicity Against Coptotermes sp. |
| title_sort | production of conidia by entomopathogenic fungi and their pathogenicity against coptotermes sp |
| topic | beauveria, biopesticide, coptotermes, entomopathogenic fungi, metarhizium |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/22435 |
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