Isolation and Screening of the Lignin-degrading Fungusand Optimization of the Mycelium Pellet Cultivation Conditions
In order to isolate andscreen microorganisms that could degrade ligninefficiently, and then constructbiological immobilization carrier, there was 1 strain of high effcient lignindegrading fungi, named G13, isolated from the sludge samples collected by the paper mill, which the highest lingin enzyme...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Harbin University of Science and Technology Publications
2019-02-01
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| Series: | Journal of Harbin University of Science and Technology |
| Subjects: | |
| Online Access: | https://hlgxb.hrbust.edu.cn/#/digest?ArticleID=1650 |
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| Summary: | In order to isolate andscreen microorganisms that could degrade ligninefficiently, and then constructbiological immobilization carrier, there was 1 strain of high effcient lignindegrading fungi, named G13, isolated from the sludge samples collected by the paper mill, which the highest lingin enzyme activity of manganese peroxidase(MnP), laccase(Lac) and lignin peroxidase(LiP) were 8.27, 0.95 and 13.55U/L, respectively. Through the study of factors that can affect the pelletization, the best conditions are as follows: sucrose concentration of 18g/L and ammonium tartrate concentration of 2.5g/L were worked as the best carbon source and nitrogen source, respectively; the most appropriate medium pH was 5; 5mL of spore suspension was inoculated on 100mL of liquid medium forpelletization (in 250mL Erlenmeyer flask);the optimum temperature was 30℃; and the most suitable shaker speed was 160r/min. Cultured under the most suitable growing conditions for 72h, the mycelium pellet formed by Aspergillus fumigatus G13 reached its maximum diameter of 3.4mm with smooth surface, excellent flexibility and hollow structure, which can be used as biological immobilization carrier as wellas meets the needs of practical application such as industrial wastewater treatment. |
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| ISSN: | 1007-2683 |