A new method for Phytophthora cactorum culturing using host plant materials to prepare agar medium
Abstract Phytophthora cactorum (Oomycota: Peronosporaceae), the agent of apple phytophthora rot, is known to infect over 200 plant species. Traditionally, P. cactorum is cultured in the laboratory on V8 juice agar medium. However, the use of commonly adopted materials for culturing P. cactorum is co...
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Nature Portfolio
2025-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-98291-7 |
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| author | Shengping Zhang Ying Cheng Jiaqiang Zhao Kuijing Liang Yupeng Pu Guoliang Xu Zhaohui Yang |
| author_facet | Shengping Zhang Ying Cheng Jiaqiang Zhao Kuijing Liang Yupeng Pu Guoliang Xu Zhaohui Yang |
| author_sort | Shengping Zhang |
| collection | DOAJ |
| description | Abstract Phytophthora cactorum (Oomycota: Peronosporaceae), the agent of apple phytophthora rot, is known to infect over 200 plant species. Traditionally, P. cactorum is cultured in the laboratory on V8 juice agar medium. However, the use of commonly adopted materials for culturing P. cactorum is costly. Theoretically, host plant materials can be utilized to develop alternative agar media for culturing plant pathogens. However, research on utilizing host plant materials to prepare agar media for culturing P. cactorum has been limited. In this study, we employed chamber assays to identify host plant species of P. cactorum through artificial inoculation. Some of the healthy host plants were then individually selected to prepare agar media for screening. Our results demonstrate that host-derived juices, such as those from celery stems or oakleaf goosefoot leaves, support stronger sporulation capacity and higher average growth rates compared to V8 medium, suggesting that host plant tissues can be effectively used to produce agar media for culturing P. cactorum. |
| format | Article |
| id | doaj-art-2dc34314ea0544f391095bc7624b3fc7 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-2dc34314ea0544f391095bc7624b3fc72025-08-20T03:18:34ZengNature PortfolioScientific Reports2045-23222025-04-0115111110.1038/s41598-025-98291-7A new method for Phytophthora cactorum culturing using host plant materials to prepare agar mediumShengping Zhang0Ying Cheng1Jiaqiang Zhao2Kuijing Liang3Yupeng Pu4Guoliang Xu5Zhaohui Yang6Institute of Shingjiazhuang Fruit Trees, Hebei Academy of Agriculture and Forestry SciencesModern Agricultural Technology Extension Center, Handan Agricultural and Rural BureauInstitute of Shingjiazhuang Fruit Trees, Hebei Academy of Agriculture and Forestry SciencesDepartment of Life Sciences, Hengshui UniversityStation of Soil and Fertilizer in Handan, Handan Agricultural and Rural BureauInstitute of Shingjiazhuang Fruit Trees, Hebei Academy of Agriculture and Forestry SciencesAgricultural Products Centre of Quality and Safety in Hebei provinceAbstract Phytophthora cactorum (Oomycota: Peronosporaceae), the agent of apple phytophthora rot, is known to infect over 200 plant species. Traditionally, P. cactorum is cultured in the laboratory on V8 juice agar medium. However, the use of commonly adopted materials for culturing P. cactorum is costly. Theoretically, host plant materials can be utilized to develop alternative agar media for culturing plant pathogens. However, research on utilizing host plant materials to prepare agar media for culturing P. cactorum has been limited. In this study, we employed chamber assays to identify host plant species of P. cactorum through artificial inoculation. Some of the healthy host plants were then individually selected to prepare agar media for screening. Our results demonstrate that host-derived juices, such as those from celery stems or oakleaf goosefoot leaves, support stronger sporulation capacity and higher average growth rates compared to V8 medium, suggesting that host plant tissues can be effectively used to produce agar media for culturing P. cactorum.https://doi.org/10.1038/s41598-025-98291-7Apple phytophthora rotPhytophthora cactorumPlant hostsAgar media |
| spellingShingle | Shengping Zhang Ying Cheng Jiaqiang Zhao Kuijing Liang Yupeng Pu Guoliang Xu Zhaohui Yang A new method for Phytophthora cactorum culturing using host plant materials to prepare agar medium Scientific Reports Apple phytophthora rot Phytophthora cactorum Plant hosts Agar media |
| title | A new method for Phytophthora cactorum culturing using host plant materials to prepare agar medium |
| title_full | A new method for Phytophthora cactorum culturing using host plant materials to prepare agar medium |
| title_fullStr | A new method for Phytophthora cactorum culturing using host plant materials to prepare agar medium |
| title_full_unstemmed | A new method for Phytophthora cactorum culturing using host plant materials to prepare agar medium |
| title_short | A new method for Phytophthora cactorum culturing using host plant materials to prepare agar medium |
| title_sort | new method for phytophthora cactorum culturing using host plant materials to prepare agar medium |
| topic | Apple phytophthora rot Phytophthora cactorum Plant hosts Agar media |
| url | https://doi.org/10.1038/s41598-025-98291-7 |
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