Optimizing protein yield from growing deer antlers
Growing antlers in deer contain bioactive compounds, most of which are proteins and peptides with effects on health, such as anticancer and regenerative properties. However, efficient extraction of these biomolecules while preserving their integrity remains a challenge. This study aimed to optimize...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1612239/full |
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| author | Nicolás Alegría-Aravena Nicolás Alegría-Aravena María-Pilar López-Garrido Josefa Quiroz-Troncoso Raquel González-Martos Marta Sánchez-Díez Clara Gavira-O’Neill Clara Gavira-O’Neill Andrés J. García-Díaz Tomás Landete-Castillejos Carmen Ramírez-Castillejo Carmen Ramírez-Castillejo Louis Chonco Francisco Sánchez-Sánchez |
| author_facet | Nicolás Alegría-Aravena Nicolás Alegría-Aravena María-Pilar López-Garrido Josefa Quiroz-Troncoso Raquel González-Martos Marta Sánchez-Díez Clara Gavira-O’Neill Clara Gavira-O’Neill Andrés J. García-Díaz Tomás Landete-Castillejos Carmen Ramírez-Castillejo Carmen Ramírez-Castillejo Louis Chonco Francisco Sánchez-Sánchez |
| author_sort | Nicolás Alegría-Aravena |
| collection | DOAJ |
| description | Growing antlers in deer contain bioactive compounds, most of which are proteins and peptides with effects on health, such as anticancer and regenerative properties. However, efficient extraction of these biomolecules while preserving their integrity remains a challenge. This study aimed to optimize the extraction of proteins from growing antlers through liquid removal methods, solvent selection, ratios, temperature, and extraction time. Lyophilization was identified as the optimal method for preserving protein integrity, particularly in biologically active regions. Among the tested solvents, water emerged as the most effective for protein extraction, achieving optimal results at a 1:10 w/v ratio with 1 hour of magnetic stirring at room temperature, although remains to be tested the anticancer effect of solvents different to water. Insulin-like growth factor 1 (IGF-1) was quantified as a key indicator of extraction efficiency, demonstrating that the optimized protocol effectively preserves this kind of bioactive protein. This methodology provides a robust framework for the extraction of proteins from growing antlers, paving the way for future applications in biomedical research. |
| format | Article |
| id | doaj-art-2dc13322d1964de2a39d7a6e4263d81c |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-2dc13322d1964de2a39d7a6e4263d81c2025-08-20T03:24:47ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-06-011310.3389/fbioe.2025.16122391612239Optimizing protein yield from growing deer antlersNicolás Alegría-Aravena0Nicolás Alegría-Aravena1María-Pilar López-Garrido2Josefa Quiroz-Troncoso3Raquel González-Martos4Marta Sánchez-Díez5Clara Gavira-O’Neill6Clara Gavira-O’Neill7Andrés J. García-Díaz8Tomás Landete-Castillejos9Carmen Ramírez-Castillejo10Carmen Ramírez-Castillejo11Louis Chonco12Francisco Sánchez-Sánchez13Instituto de Desarrollo Regional (IDR), Instituto de Recursos Cinegéticos (IREC) and Escuela Técnica Superior de Ingeniéros Agrónomos y Montes y Biotecnología (ETSIAMB), University of Castilla-La Mancha (UCLM), Albacete, SpainAsociación Española Contra el Cáncer (AECC)-Fundación Científica AECC, Albacete, SpainLaboratorio de Genética Médica, Instituto de Biomedicina (IB), Universidad de Castilla-La Mancha (UCLM), Albacete, SpainCentro de Tecnología Biomédica (CTB), Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas (ETSIAAB), Universidad Politécnica de Madrid, Madrid, SpainCentro de Tecnología Biomédica (CTB), Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas (ETSIAAB), Universidad Politécnica de Madrid, Madrid, SpainLaboratorio de Genética Médica, Instituto de Biomedicina (IB), Universidad de Castilla-La Mancha (UCLM), Albacete, SpainCentro de Tecnología Biomédica (CTB), Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas (ETSIAAB), Universidad Politécnica de Madrid, Madrid, SpainDepartment of Oncology, Instituto de Investigación Sanitaria San Carlos (IdISSC), Madrid, SpainInstituto de Desarrollo Regional (IDR), Instituto de Recursos Cinegéticos (IREC) and Escuela Técnica Superior de Ingeniéros Agrónomos y Montes y Biotecnología (ETSIAMB), University of Castilla-La Mancha (UCLM), Albacete, SpainInstituto de Desarrollo Regional (IDR), Instituto de Recursos Cinegéticos (IREC) and Escuela Técnica Superior de Ingeniéros Agrónomos y Montes y Biotecnología (ETSIAMB), University of Castilla-La Mancha (UCLM), Albacete, SpainCentro de Tecnología Biomédica (CTB), Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas (ETSIAAB), Universidad Politécnica de Madrid, Madrid, SpainDepartment of Oncology, Instituto de Investigación Sanitaria San Carlos (IdISSC), Madrid, SpainInstituto de Desarrollo Regional (IDR), Instituto de Recursos Cinegéticos (IREC) and Escuela Técnica Superior de Ingeniéros Agrónomos y Montes y Biotecnología (ETSIAMB), University of Castilla-La Mancha (UCLM), Albacete, SpainLaboratorio de Genética Médica, Instituto de Biomedicina (IB), Universidad de Castilla-La Mancha (UCLM), Albacete, SpainGrowing antlers in deer contain bioactive compounds, most of which are proteins and peptides with effects on health, such as anticancer and regenerative properties. However, efficient extraction of these biomolecules while preserving their integrity remains a challenge. This study aimed to optimize the extraction of proteins from growing antlers through liquid removal methods, solvent selection, ratios, temperature, and extraction time. Lyophilization was identified as the optimal method for preserving protein integrity, particularly in biologically active regions. Among the tested solvents, water emerged as the most effective for protein extraction, achieving optimal results at a 1:10 w/v ratio with 1 hour of magnetic stirring at room temperature, although remains to be tested the anticancer effect of solvents different to water. Insulin-like growth factor 1 (IGF-1) was quantified as a key indicator of extraction efficiency, demonstrating that the optimized protocol effectively preserves this kind of bioactive protein. This methodology provides a robust framework for the extraction of proteins from growing antlers, paving the way for future applications in biomedical research.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1612239/fullbiomedical applicationsdeer velvet antlerIGF-1optimization methodprotein extraction |
| spellingShingle | Nicolás Alegría-Aravena Nicolás Alegría-Aravena María-Pilar López-Garrido Josefa Quiroz-Troncoso Raquel González-Martos Marta Sánchez-Díez Clara Gavira-O’Neill Clara Gavira-O’Neill Andrés J. García-Díaz Tomás Landete-Castillejos Carmen Ramírez-Castillejo Carmen Ramírez-Castillejo Louis Chonco Francisco Sánchez-Sánchez Optimizing protein yield from growing deer antlers Frontiers in Bioengineering and Biotechnology biomedical applications deer velvet antler IGF-1 optimization method protein extraction |
| title | Optimizing protein yield from growing deer antlers |
| title_full | Optimizing protein yield from growing deer antlers |
| title_fullStr | Optimizing protein yield from growing deer antlers |
| title_full_unstemmed | Optimizing protein yield from growing deer antlers |
| title_short | Optimizing protein yield from growing deer antlers |
| title_sort | optimizing protein yield from growing deer antlers |
| topic | biomedical applications deer velvet antler IGF-1 optimization method protein extraction |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1612239/full |
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