PATHOLOGICAL ASSESSMENT OF LIVER EXPLANT IN PROLONGED ISCHEMIA USING THE COMBINED METHOD OF CONSERVATION IN THE EXPERIMENT
Objective – to evaluate the efficacy of ischemic protection of the combined solution based on the HTK (histidine-tryptophan-ketoglutarate) and NEPFOS (not oxygenated perfluororganic solution) in the liver explant conservation. Materials and methods. We performed a study on 60 rabbits. For histology...
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| Format: | Article |
| Language: | Russian |
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The Publishing House Medicine and Enlightenment
2016-12-01
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| Series: | Медицина в Кузбассе |
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| Online Access: | http://mednauki.ru/index.php/MK/article/view/33 |
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| author | Анатолий Сергеевич Сохарев Константин Аркадьевич Краснов Алексей Владимирович Будаев Екатерина Юрьевна Плотникова Станислав Александрович Шрайбер |
| author_facet | Анатолий Сергеевич Сохарев Константин Аркадьевич Краснов Алексей Владимирович Будаев Екатерина Юрьевна Плотникова Станислав Александрович Шрайбер |
| author_sort | Анатолий Сергеевич Сохарев |
| collection | DOAJ |
| description | Objective – to evaluate the efficacy of ischemic protection of the combined solution based on the HTK (histidine-tryptophan-ketoglutarate) and NEPFOS (not oxygenated perfluororganic solution) in the liver explant conservation.
Materials and methods. We performed a study on 60 rabbits. For histology and morphometry the biopsy of rabbit liver was performed before the conservation and in 8 hours after the conservation in different preservative solutions. Histological and morphometric studies numbered 240.
Results. In 8-hour conservation of liver explants using the combined solution based on HTK and NEPFOS less severe ischemic changes in liver tissue structures have developed compared to monopreservative solutions: standard HTK and control NEPFOS. Therefore, the combined method of conservation reduces the intensity of ischemic injury of the liver explant and thereby increases its vitality, it being the necessary condition for reducing the risk of possible post-transplant complications.
Conclusions. The use of the combined method of conservation can minimize pathological changes in the explant tissues and prevent ischemic liver injury. |
| format | Article |
| id | doaj-art-2dbba58c877342d9a2d33c10c644da54 |
| institution | Kabale University |
| issn | 1819-0901 |
| language | Russian |
| publishDate | 2016-12-01 |
| publisher | The Publishing House Medicine and Enlightenment |
| record_format | Article |
| series | Медицина в Кузбассе |
| spelling | doaj-art-2dbba58c877342d9a2d33c10c644da542025-08-20T03:28:38ZrusThe Publishing House Medicine and EnlightenmentМедицина в Кузбассе1819-09012016-12-01154465327PATHOLOGICAL ASSESSMENT OF LIVER EXPLANT IN PROLONGED ISCHEMIA USING THE COMBINED METHOD OF CONSERVATION IN THE EXPERIMENTАнатолий Сергеевич Сохарев0Константин Аркадьевич Краснов1Алексей Владимирович Будаев2Екатерина Юрьевна Плотникова3Станислав Александрович Шрайбер4МАУЗ «ГКБ № 3 им. М.А. Подгорбунского», г. КемеровоФГБОУ ВО КемГМУ Минздрава России, г. КемеровоФГБОУ ВО КемГМУ Минздрава России, г. КемеровоФГБОУ ВО КемГМУ Минздрава России, г. КемеровоГБУЗ КО ОТ «Кемеровское областное патологоанатомическое бюро»Objective – to evaluate the efficacy of ischemic protection of the combined solution based on the HTK (histidine-tryptophan-ketoglutarate) and NEPFOS (not oxygenated perfluororganic solution) in the liver explant conservation. Materials and methods. We performed a study on 60 rabbits. For histology and morphometry the biopsy of rabbit liver was performed before the conservation and in 8 hours after the conservation in different preservative solutions. Histological and morphometric studies numbered 240. Results. In 8-hour conservation of liver explants using the combined solution based on HTK and NEPFOS less severe ischemic changes in liver tissue structures have developed compared to monopreservative solutions: standard HTK and control NEPFOS. Therefore, the combined method of conservation reduces the intensity of ischemic injury of the liver explant and thereby increases its vitality, it being the necessary condition for reducing the risk of possible post-transplant complications. Conclusions. The use of the combined method of conservation can minimize pathological changes in the explant tissues and prevent ischemic liver injury.http://mednauki.ru/index.php/MK/article/view/33консервация печениишемическое повреждение печенинеоксигенированная эмульсия перфторорганических соединений |
| spellingShingle | Анатолий Сергеевич Сохарев Константин Аркадьевич Краснов Алексей Владимирович Будаев Екатерина Юрьевна Плотникова Станислав Александрович Шрайбер PATHOLOGICAL ASSESSMENT OF LIVER EXPLANT IN PROLONGED ISCHEMIA USING THE COMBINED METHOD OF CONSERVATION IN THE EXPERIMENT Медицина в Кузбассе консервация печени ишемическое повреждение печени неоксигенированная эмульсия перфторорганических соединений |
| title | PATHOLOGICAL ASSESSMENT OF LIVER EXPLANT IN PROLONGED ISCHEMIA USING THE COMBINED METHOD OF CONSERVATION IN THE EXPERIMENT |
| title_full | PATHOLOGICAL ASSESSMENT OF LIVER EXPLANT IN PROLONGED ISCHEMIA USING THE COMBINED METHOD OF CONSERVATION IN THE EXPERIMENT |
| title_fullStr | PATHOLOGICAL ASSESSMENT OF LIVER EXPLANT IN PROLONGED ISCHEMIA USING THE COMBINED METHOD OF CONSERVATION IN THE EXPERIMENT |
| title_full_unstemmed | PATHOLOGICAL ASSESSMENT OF LIVER EXPLANT IN PROLONGED ISCHEMIA USING THE COMBINED METHOD OF CONSERVATION IN THE EXPERIMENT |
| title_short | PATHOLOGICAL ASSESSMENT OF LIVER EXPLANT IN PROLONGED ISCHEMIA USING THE COMBINED METHOD OF CONSERVATION IN THE EXPERIMENT |
| title_sort | pathological assessment of liver explant in prolonged ischemia using the combined method of conservation in the experiment |
| topic | консервация печени ишемическое повреждение печени неоксигенированная эмульсия перфторорганических соединений |
| url | http://mednauki.ru/index.php/MK/article/view/33 |
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