Interactions Between Morel Cultivation, Soil Microbes, and Mineral Nutrients: Impacts and Mechanisms

Morel mushrooms (<i>Morchella</i> spp.) are prized for their nutritional and medicinal value. Despite extensive research on their cultivation, the species’ impacts on the soil microbiota and minerals remain unclear. This study systematically evaluated six <i>Morchella</i> spe...

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Main Authors: Yiwen Fu, Muxin Fan, Haiyan Qin, Zeyu Zhang, Shijun Liu, Shuwen Wu, Yun Wang, Xia Yuan
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Journal of Fungi
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Online Access:https://www.mdpi.com/2309-608X/11/6/405
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author Yiwen Fu
Muxin Fan
Haiyan Qin
Zeyu Zhang
Shijun Liu
Shuwen Wu
Yun Wang
Xia Yuan
author_facet Yiwen Fu
Muxin Fan
Haiyan Qin
Zeyu Zhang
Shijun Liu
Shuwen Wu
Yun Wang
Xia Yuan
author_sort Yiwen Fu
collection DOAJ
description Morel mushrooms (<i>Morchella</i> spp.) are prized for their nutritional and medicinal value. Despite extensive research on their cultivation, the species’ impacts on the soil microbiota and minerals remain unclear. This study systematically evaluated six <i>Morchella</i> species, analyzing their effects on soil physicochemical properties, microbial communities, and mineral nutrients levels. The results showed that yield varied significantly among the species, with an order of <i>M. sextelata</i> > <i>M. exuberans</i> > <i>M. eximia</i> > <i>M. importuna</i> > <i>Mel-13</i> > <i>Mel-21</i>. Cultivation led to a consistent reduction in soil NO<sub>3</sub><sup>−</sup>-N levels, particularly in <i>M. eximia</i> and <i>Mel-13</i> (40–50% decrease), while NH<sub>4</sub><sup>+</sup>-N levels did not change significantly, indicating mycelial nitrate preference. Mineral nutrient alterations exhibited distinct species-dependent patterns, with <i>M. eximia</i> showing the greatest increase in exchangeable Ca, while <i>M. importuna</i> and <i>M. sextelata</i> caused the most obvious decreases in available B. <i>Morchella</i> species exerted more pronounced impacts on fungal than bacterial communities, evidenced by significant reductions in alpha diversity—particularly in high-yield <i>M. sextelata</i>—suggesting species-specific fungal inhibition. At the phylum level, consistent depletion of <i>Ascomycota</i> but enrichment of <i>Mortierellomycota</i> were noted. Moreover, correlation analyses identified significant positive associations between morel yield and both fungal community diversity and exchangeable Ca content. This implies that <i>Morchella</i> species restructures soil fungal communities through nutrient competition and mineral-mediated regulation, with calcium acting as a key modulator. Overall, by elucidating the interconnected ‘<i>Morchella</i> species–microbe–mineral’ relationships, this study highlights <i>Morchella</i> species’ distinct regulation of soil microecology, providing valuable insights for the selection of optimized species like <i>M. eximia</i> and targeted soil management in morel cultivation.
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spelling doaj-art-2daa521137ce40a997662bd4b970368b2025-08-20T03:27:40ZengMDPI AGJournal of Fungi2309-608X2025-05-0111640510.3390/jof11060405Interactions Between Morel Cultivation, Soil Microbes, and Mineral Nutrients: Impacts and MechanismsYiwen Fu0Muxin Fan1Haiyan Qin2Zeyu Zhang3Shijun Liu4Shuwen Wu5Yun Wang6Xia Yuan7School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaSchool of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaSchool of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaSchool of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaSchool of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaSchool of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaSchool of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaSchool of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaMorel mushrooms (<i>Morchella</i> spp.) are prized for their nutritional and medicinal value. Despite extensive research on their cultivation, the species’ impacts on the soil microbiota and minerals remain unclear. This study systematically evaluated six <i>Morchella</i> species, analyzing their effects on soil physicochemical properties, microbial communities, and mineral nutrients levels. The results showed that yield varied significantly among the species, with an order of <i>M. sextelata</i> > <i>M. exuberans</i> > <i>M. eximia</i> > <i>M. importuna</i> > <i>Mel-13</i> > <i>Mel-21</i>. Cultivation led to a consistent reduction in soil NO<sub>3</sub><sup>−</sup>-N levels, particularly in <i>M. eximia</i> and <i>Mel-13</i> (40–50% decrease), while NH<sub>4</sub><sup>+</sup>-N levels did not change significantly, indicating mycelial nitrate preference. Mineral nutrient alterations exhibited distinct species-dependent patterns, with <i>M. eximia</i> showing the greatest increase in exchangeable Ca, while <i>M. importuna</i> and <i>M. sextelata</i> caused the most obvious decreases in available B. <i>Morchella</i> species exerted more pronounced impacts on fungal than bacterial communities, evidenced by significant reductions in alpha diversity—particularly in high-yield <i>M. sextelata</i>—suggesting species-specific fungal inhibition. At the phylum level, consistent depletion of <i>Ascomycota</i> but enrichment of <i>Mortierellomycota</i> were noted. Moreover, correlation analyses identified significant positive associations between morel yield and both fungal community diversity and exchangeable Ca content. This implies that <i>Morchella</i> species restructures soil fungal communities through nutrient competition and mineral-mediated regulation, with calcium acting as a key modulator. Overall, by elucidating the interconnected ‘<i>Morchella</i> species–microbe–mineral’ relationships, this study highlights <i>Morchella</i> species’ distinct regulation of soil microecology, providing valuable insights for the selection of optimized species like <i>M. eximia</i> and targeted soil management in morel cultivation.https://www.mdpi.com/2309-608X/11/6/405morelssoil microbessoil mineral elementssoil physicochemical properties
spellingShingle Yiwen Fu
Muxin Fan
Haiyan Qin
Zeyu Zhang
Shijun Liu
Shuwen Wu
Yun Wang
Xia Yuan
Interactions Between Morel Cultivation, Soil Microbes, and Mineral Nutrients: Impacts and Mechanisms
Journal of Fungi
morels
soil microbes
soil mineral elements
soil physicochemical properties
title Interactions Between Morel Cultivation, Soil Microbes, and Mineral Nutrients: Impacts and Mechanisms
title_full Interactions Between Morel Cultivation, Soil Microbes, and Mineral Nutrients: Impacts and Mechanisms
title_fullStr Interactions Between Morel Cultivation, Soil Microbes, and Mineral Nutrients: Impacts and Mechanisms
title_full_unstemmed Interactions Between Morel Cultivation, Soil Microbes, and Mineral Nutrients: Impacts and Mechanisms
title_short Interactions Between Morel Cultivation, Soil Microbes, and Mineral Nutrients: Impacts and Mechanisms
title_sort interactions between morel cultivation soil microbes and mineral nutrients impacts and mechanisms
topic morels
soil microbes
soil mineral elements
soil physicochemical properties
url https://www.mdpi.com/2309-608X/11/6/405
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