ACC deaminase-producing bacteria mitigate water deficit in tomato seeds

ABSTRACT: Bacteria that modulate ethylene levels through the enzyme 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase are a promising strategy to mitigate the effects of water stress. The aim of this study was to assess the effect of these bacteria on the germination and growth of tomato seedlin...

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Main Authors: Helena Souza Nascimento Santos, Regina Cássia Ferreira Ribeiro, Andréia Márcia de Souza David, José Augusto dos Santos Neto, Adelica Aparecida Xavier, João Rafael Prudêncio Santos
Format: Article
Language:English
Published: Associação Brasileira de Tecnologia de Sementes 2025-06-01
Series:Journal of Seed Science
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372025000100211&lng=en&tlng=en
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author Helena Souza Nascimento Santos
Regina Cássia Ferreira Ribeiro
Andréia Márcia de Souza David
José Augusto dos Santos Neto
Adelica Aparecida Xavier
João Rafael Prudêncio Santos
author_facet Helena Souza Nascimento Santos
Regina Cássia Ferreira Ribeiro
Andréia Márcia de Souza David
José Augusto dos Santos Neto
Adelica Aparecida Xavier
João Rafael Prudêncio Santos
author_sort Helena Souza Nascimento Santos
collection DOAJ
description ABSTRACT: Bacteria that modulate ethylene levels through the enzyme 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase are a promising strategy to mitigate the effects of water stress. The aim of this study was to assess the effect of these bacteria on the germination and growth of tomato seedlings subjected to water deficit. The experiment was conducted using a completely randomized design and 6x5 factorial scheme, with six seed treatments (four bacterial isolates and two controls) and five PEG 6000-induced osmotic potentials (0.0, -0.2, -0.4, -0.6, and -0.8 MPa), with four replications of 50 seeds. Germination, radicle protrusion, first germination count (FGC), germination speed index (GSI), and the fresh weight, root and shoot length of seedlings were evaluated. The results indicated that bacteria with ACC-deaminase activity favored germination and seedling development under water stress. Although water deficit reduced germination, isolate RZ-1 effectively mitigated these effects at -0.4 MPa. Strains E-154 and E-24 promoted the highest root and shoot growth.
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spelling doaj-art-2da6dfe3b9a8498d852dfa894c7fef4b2025-08-20T03:10:57ZengAssociação Brasileira de Tecnologia de SementesJournal of Seed Science2317-15452025-06-014710.1590/2317-1545v47292543ACC deaminase-producing bacteria mitigate water deficit in tomato seedsHelena Souza Nascimento Santoshttps://orcid.org/0000-0001-6413-3011Regina Cássia Ferreira Ribeirohttps://orcid.org/0000-0001-5138-8402Andréia Márcia de Souza Davidhttps://orcid.org/0000-0002-2747-5941José Augusto dos Santos Netohttps://orcid.org/0000-0002-8187-2411Adelica Aparecida Xavierhttps://orcid.org/0000-0001-9814-4587João Rafael Prudêncio Santoshttps://orcid.org/0000-0002-8090-9892ABSTRACT: Bacteria that modulate ethylene levels through the enzyme 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase are a promising strategy to mitigate the effects of water stress. The aim of this study was to assess the effect of these bacteria on the germination and growth of tomato seedlings subjected to water deficit. The experiment was conducted using a completely randomized design and 6x5 factorial scheme, with six seed treatments (four bacterial isolates and two controls) and five PEG 6000-induced osmotic potentials (0.0, -0.2, -0.4, -0.6, and -0.8 MPa), with four replications of 50 seeds. Germination, radicle protrusion, first germination count (FGC), germination speed index (GSI), and the fresh weight, root and shoot length of seedlings were evaluated. The results indicated that bacteria with ACC-deaminase activity favored germination and seedling development under water stress. Although water deficit reduced germination, isolate RZ-1 effectively mitigated these effects at -0.4 MPa. Strains E-154 and E-24 promoted the highest root and shoot growth.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372025000100211&lng=en&tlng=enaminocyclopropane-1-carboxylate-deaminaseethyleneosmotic potentialPEG 6000Solanum lycopersicum L
spellingShingle Helena Souza Nascimento Santos
Regina Cássia Ferreira Ribeiro
Andréia Márcia de Souza David
José Augusto dos Santos Neto
Adelica Aparecida Xavier
João Rafael Prudêncio Santos
ACC deaminase-producing bacteria mitigate water deficit in tomato seeds
Journal of Seed Science
aminocyclopropane-1-carboxylate-deaminase
ethylene
osmotic potential
PEG 6000
Solanum lycopersicum L
title ACC deaminase-producing bacteria mitigate water deficit in tomato seeds
title_full ACC deaminase-producing bacteria mitigate water deficit in tomato seeds
title_fullStr ACC deaminase-producing bacteria mitigate water deficit in tomato seeds
title_full_unstemmed ACC deaminase-producing bacteria mitigate water deficit in tomato seeds
title_short ACC deaminase-producing bacteria mitigate water deficit in tomato seeds
title_sort acc deaminase producing bacteria mitigate water deficit in tomato seeds
topic aminocyclopropane-1-carboxylate-deaminase
ethylene
osmotic potential
PEG 6000
Solanum lycopersicum L
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372025000100211&lng=en&tlng=en
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