Global analysis of transcriptome and metabolomics revealed that fatty acid synthesis and urea cycle process regulate the growth of chinese soft-shelled turtle
The Chinese soft-shelled turtle (Pelodiscus sinensis) is an economically important aquatic species, with its growth influenced by genetic factors, environmental conditions, nutritional status, and intestinal microbiota. Despite its slow growth rate, typically taking 2–3 years to reach market size, u...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-07-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425002005 |
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| author | Tong Zhou Guobin Chen Lihuan Cao Jizeng Cao Huizi Ji Guiwei Zou Hongwei Liang |
| author_facet | Tong Zhou Guobin Chen Lihuan Cao Jizeng Cao Huizi Ji Guiwei Zou Hongwei Liang |
| author_sort | Tong Zhou |
| collection | DOAJ |
| description | The Chinese soft-shelled turtle (Pelodiscus sinensis) is an economically important aquatic species, with its growth influenced by genetic factors, environmental conditions, nutritional status, and intestinal microbiota. Despite its slow growth rate, typically taking 2–3 years to reach market size, understanding the molecular mechanisms that regulate its growth is critical for optimizing breeding conditions and improving aquaculture sustainability. In this study, we carried out a multi-omics analysis in individuals of P. sinensis aged 2, 5, and 8 months to systematically disclose the biological mechanisms and explore how these mechanisms affect its growth and adaptive ability. We found significant changes in the composition of intestinal bacteria, with Fusobacteriota and Proteobacteria dominating at the phylum level and displaying increasingly intimate interactions as growth processed. Notable enrichments were observed in pathways related to arginine biosynthesis and glycine and serine metabolism in differentially expressed genes (DEGs), as well as steroid biosynthesis and steroid hormone biosynthesis pathways in differentially expressed metabolites (DEMs). Furthermore, fatty acid synthesis and the urea cycle were identified as key metabolic pathways essential for the breeding of Chinese soft-shelled turtles. This research provides insights that can be utilized to optimize feed formulations, enhance breeding efficiency, and promote the healthy growth of P. sinensis in aquaculture. |
| format | Article |
| id | doaj-art-2da226b792824b98b8a877cf57a7d203 |
| institution | OA Journals |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-2da226b792824b98b8a877cf57a7d2032025-08-20T02:31:12ZengElsevierAquaculture Reports2352-51342025-07-014210281410.1016/j.aqrep.2025.102814Global analysis of transcriptome and metabolomics revealed that fatty acid synthesis and urea cycle process regulate the growth of chinese soft-shelled turtleTong Zhou0Guobin Chen1Lihuan Cao2Jizeng Cao3Huizi Ji4Guiwei Zou5Hongwei Liang6Yangtze River Fisheries Research Institute, Chinese Academy of Fisheries Science, Wuhan 430223, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fisheries Science, Wuhan 430223, China; State Key Laboratory of Marine Environmental Science & College of Ocean and Earth Sciences, Xiamen University, Xiamen, China; College of Ocean and Earth Sciences, Xiamen University, Xiamen 361000, ChinaLeshan normal University, Leshan 614000, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fisheries Science, Wuhan 430223, China; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fisheries Science, Wuhan 430223, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fisheries Science, Wuhan 430223, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fisheries Science, Wuhan 430223, China; Corresponding author.The Chinese soft-shelled turtle (Pelodiscus sinensis) is an economically important aquatic species, with its growth influenced by genetic factors, environmental conditions, nutritional status, and intestinal microbiota. Despite its slow growth rate, typically taking 2–3 years to reach market size, understanding the molecular mechanisms that regulate its growth is critical for optimizing breeding conditions and improving aquaculture sustainability. In this study, we carried out a multi-omics analysis in individuals of P. sinensis aged 2, 5, and 8 months to systematically disclose the biological mechanisms and explore how these mechanisms affect its growth and adaptive ability. We found significant changes in the composition of intestinal bacteria, with Fusobacteriota and Proteobacteria dominating at the phylum level and displaying increasingly intimate interactions as growth processed. Notable enrichments were observed in pathways related to arginine biosynthesis and glycine and serine metabolism in differentially expressed genes (DEGs), as well as steroid biosynthesis and steroid hormone biosynthesis pathways in differentially expressed metabolites (DEMs). Furthermore, fatty acid synthesis and the urea cycle were identified as key metabolic pathways essential for the breeding of Chinese soft-shelled turtles. This research provides insights that can be utilized to optimize feed formulations, enhance breeding efficiency, and promote the healthy growth of P. sinensis in aquaculture.http://www.sciencedirect.com/science/article/pii/S2352513425002005GrowthPelodiscus sinensisGrowth and DevelopmentMulti-omics analysisIntestine microbiota |
| spellingShingle | Tong Zhou Guobin Chen Lihuan Cao Jizeng Cao Huizi Ji Guiwei Zou Hongwei Liang Global analysis of transcriptome and metabolomics revealed that fatty acid synthesis and urea cycle process regulate the growth of chinese soft-shelled turtle Aquaculture Reports Growth Pelodiscus sinensis Growth and Development Multi-omics analysis Intestine microbiota |
| title | Global analysis of transcriptome and metabolomics revealed that fatty acid synthesis and urea cycle process regulate the growth of chinese soft-shelled turtle |
| title_full | Global analysis of transcriptome and metabolomics revealed that fatty acid synthesis and urea cycle process regulate the growth of chinese soft-shelled turtle |
| title_fullStr | Global analysis of transcriptome and metabolomics revealed that fatty acid synthesis and urea cycle process regulate the growth of chinese soft-shelled turtle |
| title_full_unstemmed | Global analysis of transcriptome and metabolomics revealed that fatty acid synthesis and urea cycle process regulate the growth of chinese soft-shelled turtle |
| title_short | Global analysis of transcriptome and metabolomics revealed that fatty acid synthesis and urea cycle process regulate the growth of chinese soft-shelled turtle |
| title_sort | global analysis of transcriptome and metabolomics revealed that fatty acid synthesis and urea cycle process regulate the growth of chinese soft shelled turtle |
| topic | Growth Pelodiscus sinensis Growth and Development Multi-omics analysis Intestine microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425002005 |
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