Genotype B of deformed wing virus and related recombinant viruses become dominant in European honey bee colonies
Abstract The Varroa destructor mite’s transmission of deformed wing virus (DWV) to honey bees is responsible for most winter mortalities of colonies worldwide. Four DWV genotypes (A, B, C and D) and numerous recombinants have been described. The most recent studies have reported the greater prevalen...
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Nature Portfolio
2025-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-86937-5 |
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| author | Fabrice Sircoulomb Eric Dubois Frank Schurr Pierrick Lucas Marina Meixner Alicia Bertolotti Yannick Blanchard Richard Thiéry |
| author_facet | Fabrice Sircoulomb Eric Dubois Frank Schurr Pierrick Lucas Marina Meixner Alicia Bertolotti Yannick Blanchard Richard Thiéry |
| author_sort | Fabrice Sircoulomb |
| collection | DOAJ |
| description | Abstract The Varroa destructor mite’s transmission of deformed wing virus (DWV) to honey bees is responsible for most winter mortalities of colonies worldwide. Four DWV genotypes (A, B, C and D) and numerous recombinants have been described. The most recent studies have reported the greater prevalence of DWV-B over DWV-A in several countries, including European ones, while C and D genotypes appear rare or extinct. However, no global evaluation of DWV-A and DWV-B distribution was available at the European level to date. In this study, we quantified both DWV genotypes by real-time PCR from pools or individual honey bees and from V. destructor mites sampled in 15 European countries between 2010 and 2017. These data and the sequencing of the viral RNA provide a first insight into DWV diversity, with a clear dominance of DWV-B and recombinants (A/B) in Europe. Chimeric sequencing reads were used to locate the recombinant junctions along the DWV genome. These were not randomly distributed, but mainly clustered in three genomic areas: the 5’UTR, leader peptide and helicase coding sequences. In our study, the DWV recombinant genomes shared at least the VP1-VP3 coding sequences with the DWV-B. Further studies are needed to explore the apicultural context explaining these differences in DWV genotype dominance. |
| format | Article |
| id | doaj-art-2d9ff2976bf34f67b8fa8e5b2e801e98 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-2d9ff2976bf34f67b8fa8e5b2e801e982025-02-09T12:28:58ZengNature PortfolioScientific Reports2045-23222025-02-0115111510.1038/s41598-025-86937-5Genotype B of deformed wing virus and related recombinant viruses become dominant in European honey bee coloniesFabrice Sircoulomb0Eric Dubois1Frank Schurr2Pierrick Lucas3Marina Meixner4Alicia Bertolotti5Yannick Blanchard6Richard Thiéry7ANSES Sophia Antipolis Laboratory, Unit of Honey Bee PathologyANSES Sophia Antipolis Laboratory, Unit of Honey Bee PathologyANSES Sophia Antipolis Laboratory, Unit of Honey Bee PathologyANSES Ploufragan – Plouzané – Niort Laboratory, Unit of Viral Genetics and BiosecurityLLH Bee Institute KirchhainANSES Sophia Antipolis Laboratory, Unit of Honey Bee PathologyANSES Ploufragan – Plouzané – Niort Laboratory, Unit of Viral Genetics and BiosecurityANSES Sophia Antipolis Laboratory, Unit of Honey Bee PathologyAbstract The Varroa destructor mite’s transmission of deformed wing virus (DWV) to honey bees is responsible for most winter mortalities of colonies worldwide. Four DWV genotypes (A, B, C and D) and numerous recombinants have been described. The most recent studies have reported the greater prevalence of DWV-B over DWV-A in several countries, including European ones, while C and D genotypes appear rare or extinct. However, no global evaluation of DWV-A and DWV-B distribution was available at the European level to date. In this study, we quantified both DWV genotypes by real-time PCR from pools or individual honey bees and from V. destructor mites sampled in 15 European countries between 2010 and 2017. These data and the sequencing of the viral RNA provide a first insight into DWV diversity, with a clear dominance of DWV-B and recombinants (A/B) in Europe. Chimeric sequencing reads were used to locate the recombinant junctions along the DWV genome. These were not randomly distributed, but mainly clustered in three genomic areas: the 5’UTR, leader peptide and helicase coding sequences. In our study, the DWV recombinant genomes shared at least the VP1-VP3 coding sequences with the DWV-B. Further studies are needed to explore the apicultural context explaining these differences in DWV genotype dominance.https://doi.org/10.1038/s41598-025-86937-5 |
| spellingShingle | Fabrice Sircoulomb Eric Dubois Frank Schurr Pierrick Lucas Marina Meixner Alicia Bertolotti Yannick Blanchard Richard Thiéry Genotype B of deformed wing virus and related recombinant viruses become dominant in European honey bee colonies Scientific Reports |
| title | Genotype B of deformed wing virus and related recombinant viruses become dominant in European honey bee colonies |
| title_full | Genotype B of deformed wing virus and related recombinant viruses become dominant in European honey bee colonies |
| title_fullStr | Genotype B of deformed wing virus and related recombinant viruses become dominant in European honey bee colonies |
| title_full_unstemmed | Genotype B of deformed wing virus and related recombinant viruses become dominant in European honey bee colonies |
| title_short | Genotype B of deformed wing virus and related recombinant viruses become dominant in European honey bee colonies |
| title_sort | genotype b of deformed wing virus and related recombinant viruses become dominant in european honey bee colonies |
| url | https://doi.org/10.1038/s41598-025-86937-5 |
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