RhD-negative blood donors: genetic polymorphisms and testing strategies
[Objective] To investigate the genetic basis of RhD-negative phenotype in the blood donor population of Nantong City. [Methods] RHD genotyping was performed on 386 randomly selected RhD-negative donor samples (from a total of 676 RhD-negative donors identified between January 20, 2023, and June 28,...
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Institute of Blood Transfusion of Chinese Academy of Medical Sciences
2025-07-01
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| Series: | Zhongguo shuxue zazhi |
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| Online Access: | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.07.011&lang=en |
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| _version_ | 1849685025171177472 |
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| author | WANG Kecheng WANG Xiaoqin DING Yingzhou ZHANG Tingting LIU Ming XU Cheng |
| author_facet | WANG Kecheng WANG Xiaoqin DING Yingzhou ZHANG Tingting LIU Ming XU Cheng |
| author_sort | WANG Kecheng |
| collection | DOAJ |
| description | [Objective] To investigate the genetic basis of RhD-negative phenotype in the blood donor population of Nantong City. [Methods] RHD genotyping was performed on 386 randomly selected RhD-negative donor samples (from a total of 676 RhD-negative donors identified between January 20, 2023, and June 28, 2024) using polymerase chain reaction (PCR), and the inconclusive results were confirmed by nucleotide sequencing. [Results] Ten RHD allele types were identified: The complete deletion variant RHD* 01N.01 was predominant (64.25%, 248/386); followed by RHD* 01EL.01 (19.69%, 76/386). RHD* 01N.03, RHD* 01N.04, RHD* 01N.16 and RHD* 01EL.32 were frequently observed., RHD* 01EL.02, RHD* 01EL.08, RHD* 01EL.37 and RHD* 01N.25 were rare, and two exon deletion variants remained uncharacterized. The phenotypic distribution of RhD-negative blood donors was ccee (55.44%)>Ccee(31.09%)>ccEe(5.96%)>CCee(5.44%)>CcEe(1.81%)>CcEE(0.26%), and the antigen distribution trend was e(99.74%)>c(94.56%)>C(38.60%)>E(8.03%). A correlation was observed between RHD genotypes and RhCE phenotypes. [Conclusion] The Nantong blood donor population exhibits unique RHD gene polymorphisms. Integrating RhCE serological phenotyping with RHD genotyping is essential for ensuring transfusion safety. |
| format | Article |
| id | doaj-art-2d9dc18337454dc5bb18ca1bfe72d5f2 |
| institution | DOAJ |
| issn | 1004-549X |
| language | zho |
| publishDate | 2025-07-01 |
| publisher | Institute of Blood Transfusion of Chinese Academy of Medical Sciences |
| record_format | Article |
| series | Zhongguo shuxue zazhi |
| spelling | doaj-art-2d9dc18337454dc5bb18ca1bfe72d5f22025-08-20T03:23:16ZzhoInstitute of Blood Transfusion of Chinese Academy of Medical SciencesZhongguo shuxue zazhi1004-549X2025-07-0138793494010.13303/j.cjbt.issn.1004-549x.2025.07.0111004-549X(2025)7-0934-07RhD-negative blood donors: genetic polymorphisms and testing strategiesWANG Kecheng0WANG Xiaoqin1DING Yingzhou2ZHANG Tingting3LIU Ming4XU Cheng5Nantong Blood Center, Nantong 226014, ChinaNantong Blood Center, Nantong 226014, ChinaNantong Blood Center, Nantong 226014, ChinaWeihe Biotechnology Inc, Taizhou 225300, ChinaWeihe Biotechnology Inc, Taizhou 225300, ChinaNantong Blood Center, Nantong 226014, China[Objective] To investigate the genetic basis of RhD-negative phenotype in the blood donor population of Nantong City. [Methods] RHD genotyping was performed on 386 randomly selected RhD-negative donor samples (from a total of 676 RhD-negative donors identified between January 20, 2023, and June 28, 2024) using polymerase chain reaction (PCR), and the inconclusive results were confirmed by nucleotide sequencing. [Results] Ten RHD allele types were identified: The complete deletion variant RHD* 01N.01 was predominant (64.25%, 248/386); followed by RHD* 01EL.01 (19.69%, 76/386). RHD* 01N.03, RHD* 01N.04, RHD* 01N.16 and RHD* 01EL.32 were frequently observed., RHD* 01EL.02, RHD* 01EL.08, RHD* 01EL.37 and RHD* 01N.25 were rare, and two exon deletion variants remained uncharacterized. The phenotypic distribution of RhD-negative blood donors was ccee (55.44%)>Ccee(31.09%)>ccEe(5.96%)>CCee(5.44%)>CcEe(1.81%)>CcEE(0.26%), and the antigen distribution trend was e(99.74%)>c(94.56%)>C(38.60%)>E(8.03%). A correlation was observed between RHD genotypes and RhCE phenotypes. [Conclusion] The Nantong blood donor population exhibits unique RHD gene polymorphisms. Integrating RhCE serological phenotyping with RHD genotyping is essential for ensuring transfusion safety.https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.07.011&lang=enrh blood groupphenotypegenotyperhd negativedelrhd variantsgenetic basis |
| spellingShingle | WANG Kecheng WANG Xiaoqin DING Yingzhou ZHANG Tingting LIU Ming XU Cheng RhD-negative blood donors: genetic polymorphisms and testing strategies Zhongguo shuxue zazhi rh blood group phenotype genotype rhd negative del rhd variants genetic basis |
| title | RhD-negative blood donors: genetic polymorphisms and testing strategies |
| title_full | RhD-negative blood donors: genetic polymorphisms and testing strategies |
| title_fullStr | RhD-negative blood donors: genetic polymorphisms and testing strategies |
| title_full_unstemmed | RhD-negative blood donors: genetic polymorphisms and testing strategies |
| title_short | RhD-negative blood donors: genetic polymorphisms and testing strategies |
| title_sort | rhd negative blood donors genetic polymorphisms and testing strategies |
| topic | rh blood group phenotype genotype rhd negative del rhd variants genetic basis |
| url | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.07.011&lang=en |
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