Isolation, Molecular Identification, and Antibiotic Resistance Profile of Salmonella Typhimurium Isolated from Calves Fecal Samples of Dairy Farms in Hamedan
Introduction: Calf diarrhea is one of the significant problems in dairy farms associated with treatment costs and reduced livestock production. Salmonellae are among the most common and the major causative agents of diarrhea in calves and humans. The present study was carried out to isolate and...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Pasteur Institute of Iran
2022-03-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
| Subjects: | |
| Online Access: | https://jommid.pasteur.ac.ir/article-1-441-en.html |
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| Summary: | Introduction: Calf diarrhea is one of the significant problems in dairy farms
associated with treatment costs and reduced livestock production.
Salmonellae are among the most common and the major causative agents of
diarrhea in calves and humans. The present study was carried out to isolate
and identify Salmonella in fecal samples of calves in industrial dairy farms
of Hamedan and to determine antibiotic resistance profiles of the probable
isolates. Methods: Salmonella were presumptively isolated based on the
cultural characteristics and biochemical tests, and the identity of the isolates
was further confirmed using genus- and serotype-specific PCR assays. Kirby-
Bauer disk diffusion method was performed to determine antibiotic
resistance profiles of the isolates. Results: Out of 120 stool samples collected
from 8 industrial farms, 22 (18.33%) isolates possessing rfbJ, fliC and fljB
genes were identified as Salmonella Typhimurium serotype. Antibiotic
susceptibility test revealed all isolates (100%) were susceptible to
gentamicin, amoxicillin-clavulanic acid, kanamycin, and ciprofloxacin and
resistant against cotrimoxazole, cefazolin, and cefixime. Conclusion: To our
knowledge, this study was the first report of Salmonella infection in
Hamedan's dairy farms, indicating a relatively high prevalence rate of S.
Typhimurium infection as the only detected serotype. Antibiotic resistance
should also be considered a severe public health concern. Thus, effective
hygiene measures should be adopted to prevent or reduce the infection, and
monitoring antibiotic susceptibility is required to choose the drug of choice
for treatment. |
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| ISSN: | 2345-5349 2345-5330 |