Characteristics of serum bile acid profiles among individuals with metabolic dysfunction-associated steatotic liver disease

Abstract Background Metabolic dysfunction-associated steatotic liver disease (MASLD) has become the predominant chronic liver condition globally. Bile acid (BA) metabolism contributes significantly to MASLD progression. In this multicenter clinical study, we aimed to characterize serum BA profiles i...

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Main Authors: Sheng Lyu, Jiani Yang, Xin Xin, Qinmei Sun, Beiyu Cai, Xin Wang, Ziming An, Jian Sun, Yiyang Hu, Lei Shi, Qin Feng, Xiaojun Gou
Format: Article
Language:English
Published: BMC 2025-05-01
Series:BMC Gastroenterology
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Online Access:https://doi.org/10.1186/s12876-025-03903-1
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author Sheng Lyu
Jiani Yang
Xin Xin
Qinmei Sun
Beiyu Cai
Xin Wang
Ziming An
Jian Sun
Yiyang Hu
Lei Shi
Qin Feng
Xiaojun Gou
author_facet Sheng Lyu
Jiani Yang
Xin Xin
Qinmei Sun
Beiyu Cai
Xin Wang
Ziming An
Jian Sun
Yiyang Hu
Lei Shi
Qin Feng
Xiaojun Gou
author_sort Sheng Lyu
collection DOAJ
description Abstract Background Metabolic dysfunction-associated steatotic liver disease (MASLD) has become the predominant chronic liver condition globally. Bile acid (BA) metabolism contributes significantly to MASLD progression. In this multicenter clinical study, we aimed to characterize serum BA profiles in patients with MASLD and identify specific alterations compared to healthy controls. Methods All MASLD cases were sourced from the gastroenterology outpatient departments of Shanghai Baoshan Hospital of Integrated Chinese and Western Medicine, Shanghai Baoshan District Songnan Community Health Service Center, and Lianyungang Oriental Hospital between June 2015 and December 2019. The data were analyzed using SPSS version 26.0, with a p-value of less than 0.05 considered significant. Results A total of 215 participants (35.3% women) with MASLD and 49 controls (44.9% women), aged 18–65 years, were included. MASLD patients showed higher levels of serum total BA (TBA), cholic acid (CA), chenodeoxycholic acid (CDCA), and ursodeoxycholic acid (UDCA) (p < 0.05, p < 0.01) when compared to controls. Furthermore, women patients with MASLD demonstrated notably higher levels of lithocholic acid (LCA), glycolithocholic acid (GLCA), and taurolithocholic acid (TLCA) than men patients with MASLD (p < 0.025, p < 0.01). Compared to women, men exhibited a higher proportion of primary to secondary BAs. Additionally, in men patients with MASLD, the serum concentrations of CA, CDCA, glycocholic acid (GCA), glycochenodeoxycholic acid (GCDCA), and taurochenodeoxycholic acid (TCDCA) exhibited significant negative correlations with ALT levels, while deoxycholic acid (DCA) and TLCA showed negative correlations with BMI. Conclusions Patients with MASLD exhibited notable variations in BA profiles, including sex-specific differences. This study provides corresponding evidence on the association between BAs and MASLD. Trial registration Chinese Clinical Trial Registry, NO: ChiCTR-OOC-15006157, registration date: March 25, 2015.
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spelling doaj-art-2d9722dd983b4da59c4f988a28b31ece2025-08-20T03:09:19ZengBMCBMC Gastroenterology1471-230X2025-05-0125111110.1186/s12876-025-03903-1Characteristics of serum bile acid profiles among individuals with metabolic dysfunction-associated steatotic liver diseaseSheng Lyu0Jiani Yang1Xin Xin2Qinmei Sun3Beiyu Cai4Xin Wang5Ziming An6Jian Sun7Yiyang Hu8Lei Shi9Qin Feng10Xiaojun Gou11Institute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineInstitute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineInstitute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineInstitute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineInstitute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineInstitute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineInstitute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineClinical Laboratory, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineInstitute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineClinical Laboratory, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineInstitute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineBaoshan District Hospital of Integrated Traditional Chinese and Western Medicine of ShanghaiAbstract Background Metabolic dysfunction-associated steatotic liver disease (MASLD) has become the predominant chronic liver condition globally. Bile acid (BA) metabolism contributes significantly to MASLD progression. In this multicenter clinical study, we aimed to characterize serum BA profiles in patients with MASLD and identify specific alterations compared to healthy controls. Methods All MASLD cases were sourced from the gastroenterology outpatient departments of Shanghai Baoshan Hospital of Integrated Chinese and Western Medicine, Shanghai Baoshan District Songnan Community Health Service Center, and Lianyungang Oriental Hospital between June 2015 and December 2019. The data were analyzed using SPSS version 26.0, with a p-value of less than 0.05 considered significant. Results A total of 215 participants (35.3% women) with MASLD and 49 controls (44.9% women), aged 18–65 years, were included. MASLD patients showed higher levels of serum total BA (TBA), cholic acid (CA), chenodeoxycholic acid (CDCA), and ursodeoxycholic acid (UDCA) (p < 0.05, p < 0.01) when compared to controls. Furthermore, women patients with MASLD demonstrated notably higher levels of lithocholic acid (LCA), glycolithocholic acid (GLCA), and taurolithocholic acid (TLCA) than men patients with MASLD (p < 0.025, p < 0.01). Compared to women, men exhibited a higher proportion of primary to secondary BAs. Additionally, in men patients with MASLD, the serum concentrations of CA, CDCA, glycocholic acid (GCA), glycochenodeoxycholic acid (GCDCA), and taurochenodeoxycholic acid (TCDCA) exhibited significant negative correlations with ALT levels, while deoxycholic acid (DCA) and TLCA showed negative correlations with BMI. Conclusions Patients with MASLD exhibited notable variations in BA profiles, including sex-specific differences. This study provides corresponding evidence on the association between BAs and MASLD. Trial registration Chinese Clinical Trial Registry, NO: ChiCTR-OOC-15006157, registration date: March 25, 2015.https://doi.org/10.1186/s12876-025-03903-1Metabolic dysfunction-associated steatotic liver diseaseBile acidSex
spellingShingle Sheng Lyu
Jiani Yang
Xin Xin
Qinmei Sun
Beiyu Cai
Xin Wang
Ziming An
Jian Sun
Yiyang Hu
Lei Shi
Qin Feng
Xiaojun Gou
Characteristics of serum bile acid profiles among individuals with metabolic dysfunction-associated steatotic liver disease
BMC Gastroenterology
Metabolic dysfunction-associated steatotic liver disease
Bile acid
Sex
title Characteristics of serum bile acid profiles among individuals with metabolic dysfunction-associated steatotic liver disease
title_full Characteristics of serum bile acid profiles among individuals with metabolic dysfunction-associated steatotic liver disease
title_fullStr Characteristics of serum bile acid profiles among individuals with metabolic dysfunction-associated steatotic liver disease
title_full_unstemmed Characteristics of serum bile acid profiles among individuals with metabolic dysfunction-associated steatotic liver disease
title_short Characteristics of serum bile acid profiles among individuals with metabolic dysfunction-associated steatotic liver disease
title_sort characteristics of serum bile acid profiles among individuals with metabolic dysfunction associated steatotic liver disease
topic Metabolic dysfunction-associated steatotic liver disease
Bile acid
Sex
url https://doi.org/10.1186/s12876-025-03903-1
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