Methylation of the tumor associated genes in head and neck squamous cell carcinoma
Introduction. Head and neck cancer is a heterogenous group of malignant tumors of different etiologies, molecular mechanisms of which are still insufficiently studied.Aim. Investigation of DNA methylation status of some tumor associated genes (RASSF1A, RASSF2, RASSF5, CDO1, MEST and WIF1) in patient...
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| Format: | Article |
| Language: | Russian |
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ABV-press
2023-03-01
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| Series: | Опухоли головы и шеи |
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| Online Access: | https://ogsh.abvpress.ru/jour/article/view/845 |
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| author | S. V. Kurevlev L. V. Tskhovrebova A. V. Aghajanyan T. Kh. Fatkhudinov K. B. Gordon M. M. Azova |
| author_facet | S. V. Kurevlev L. V. Tskhovrebova A. V. Aghajanyan T. Kh. Fatkhudinov K. B. Gordon M. M. Azova |
| author_sort | S. V. Kurevlev |
| collection | DOAJ |
| description | Introduction. Head and neck cancer is a heterogenous group of malignant tumors of different etiologies, molecular mechanisms of which are still insufficiently studied.Aim. Investigation of DNA methylation status of some tumor associated genes (RASSF1A, RASSF2, RASSF5, CDO1, MEST and WIF1) in patients with head and neck squamous cell carcinoma.Materials and methods. The DNA methylation level of normal and tumor tissues was analyzed using bisulfite conversion and methylation-sensitive high-resolution melting in 25 patients (21 men and 4 women) diagnosed with neck squamous cell carcinoma.Results. There were significant differences in levels of DNA methylation between tumor and normal tissues in the CDO1 and WIF1 genes in all groups and subgroups of patients (larynx and other cancers, squamous cell carcinoma keratinizing and non-keratinizing, primary and recurrent tumor, smokers and non-smokers). The methylation level in the CDO1 gene in tumor tissue was significantly increased in the T4 and T3 stage subgroups compared to T2.Conclusion. The increased level of methylation of the CDO1 and WIF1 genes, as well as changes in their expression are among the molecular mechanisms involved in the neck squamous cell carcinoma development. They can be considered as prognostic and diagnostic markers for this pathology. |
| format | Article |
| id | doaj-art-2d92b38bcecd4bc2ae3855574fe44b24 |
| institution | Kabale University |
| issn | 2222-1468 2411-4634 |
| language | Russian |
| publishDate | 2023-03-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Опухоли головы и шеи |
| spelling | doaj-art-2d92b38bcecd4bc2ae3855574fe44b242025-08-20T03:37:42ZrusABV-pressОпухоли головы и шеи2222-14682411-46342023-03-01124617010.17650/2222-1468-2022-12-4-61-70525Methylation of the tumor associated genes in head and neck squamous cell carcinomaS. V. Kurevlev0L. V. Tskhovrebova1A. V. Aghajanyan2T. Kh. Fatkhudinov3K. B. Gordon4M. M. Azova5Рeoples’ Friendship University of RussiaРeoples’ Friendship University of RussiaРeoples’ Friendship University of RussiaРeoples’ Friendship University of RussiaРeoples’ Friendship University of Russia; A.F. Tsyb Medical Radiological Research Center – branch of the National Medical Research Center of Radiology, Ministry of Health of RussiaРeoples’ Friendship University of RussiaIntroduction. Head and neck cancer is a heterogenous group of malignant tumors of different etiologies, molecular mechanisms of which are still insufficiently studied.Aim. Investigation of DNA methylation status of some tumor associated genes (RASSF1A, RASSF2, RASSF5, CDO1, MEST and WIF1) in patients with head and neck squamous cell carcinoma.Materials and methods. The DNA methylation level of normal and tumor tissues was analyzed using bisulfite conversion and methylation-sensitive high-resolution melting in 25 patients (21 men and 4 women) diagnosed with neck squamous cell carcinoma.Results. There were significant differences in levels of DNA methylation between tumor and normal tissues in the CDO1 and WIF1 genes in all groups and subgroups of patients (larynx and other cancers, squamous cell carcinoma keratinizing and non-keratinizing, primary and recurrent tumor, smokers and non-smokers). The methylation level in the CDO1 gene in tumor tissue was significantly increased in the T4 and T3 stage subgroups compared to T2.Conclusion. The increased level of methylation of the CDO1 and WIF1 genes, as well as changes in their expression are among the molecular mechanisms involved in the neck squamous cell carcinoma development. They can be considered as prognostic and diagnostic markers for this pathology.https://ogsh.abvpress.ru/jour/article/view/845dna methylationtumor-associated geneshead and neck squamous cell carcinoma |
| spellingShingle | S. V. Kurevlev L. V. Tskhovrebova A. V. Aghajanyan T. Kh. Fatkhudinov K. B. Gordon M. M. Azova Methylation of the tumor associated genes in head and neck squamous cell carcinoma Опухоли головы и шеи dna methylation tumor-associated genes head and neck squamous cell carcinoma |
| title | Methylation of the tumor associated genes in head and neck squamous cell carcinoma |
| title_full | Methylation of the tumor associated genes in head and neck squamous cell carcinoma |
| title_fullStr | Methylation of the tumor associated genes in head and neck squamous cell carcinoma |
| title_full_unstemmed | Methylation of the tumor associated genes in head and neck squamous cell carcinoma |
| title_short | Methylation of the tumor associated genes in head and neck squamous cell carcinoma |
| title_sort | methylation of the tumor associated genes in head and neck squamous cell carcinoma |
| topic | dna methylation tumor-associated genes head and neck squamous cell carcinoma |
| url | https://ogsh.abvpress.ru/jour/article/view/845 |
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