Characteristics of the vaginal microbiota and vaginal metabolites in women with cervical dysplasia
IntroductionEmerging evidence suggests that the vaginal microbiota is closely associated with cervical cancer. However, little is known about the relationships among the vaginal microbiota, vaginal metabolites, and cervical lesion progression in women undergoing cervical dysplasia.MethodsIn this stu...
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Frontiers Media S.A.
2024-10-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1457216/full |
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| author | Tiantian Yu Tiantian Yu Shan Gao Fen Jin Bingbing Yan Wendong Wang Zhongmin Wang |
| author_facet | Tiantian Yu Tiantian Yu Shan Gao Fen Jin Bingbing Yan Wendong Wang Zhongmin Wang |
| author_sort | Tiantian Yu |
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| description | IntroductionEmerging evidence suggests that the vaginal microbiota is closely associated with cervical cancer. However, little is known about the relationships among the vaginal microbiota, vaginal metabolites, and cervical lesion progression in women undergoing cervical dysplasia.MethodsIn this study, to understand vaginal microbiota signatures and vaginal metabolite changes in women with cervical lesions of different grades and cancer, individuals with normal or cervical dysplasia were recruited and divided into healthy controls (HC) group, low-grade squamous intraepithelial lesions (LSIL) group, high-grade squamous intraepithelial lesions (HSIL) group, and cervical cancer (CC) group. Vaginal secretion samples were collected for 16S rRNA gene sequencing, liquid chromatography coupled with mass spectrometry (LC–MS)-based metabolomics, and integrated analysis.ResultsThe results demonstrated that bacterial richness and diversity were greater in the CC group than the other three groups. Additionally, Lactobacillus was found to be negatively associated with bacterial diversity and bacterial metabolic functions, which increased with the degree of cervical lesions and cancer. Metabolomic analysis revealed that distinct metabolites were enriched in these metabolite pathways, including tryptophan metabolism, retinol metabolism, glutathione metabolism, alanine, aspartate, and glutamate metabolism, as well as citrate cycle (TCA cycle). Correlation analysis revealed positive associations between CC group-decreased Lactobacillus abundance and CC group-decreased metabolites. Lactobacillus iners was both negative to nadB and kynU genes, the predicted abundance of which was significantly higher in the CC group. The linear regression model showed that the combination of the vaginal microbiota and vaginal metabolites has good diagnostic performance for cervical cancer.DiscussionOur results indicated a clear difference in the vaginal microbiota and vaginal metabolites of women with cervical dysplasia. Specifically altered bacteria and metabolites were closely associated with the degree of cervical lesions and cancer, indicating the potential of the vaginal microbiota and vaginal metabolites as modifiable factors and therapeutic targets for preventing cervical cancer. |
| format | Article |
| id | doaj-art-2d8bcd0a1f624974a3b01077a362f5c2 |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-2d8bcd0a1f624974a3b01077a362f5c22024-11-19T10:09:57ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882024-10-011410.3389/fcimb.2024.14572161457216Characteristics of the vaginal microbiota and vaginal metabolites in women with cervical dysplasiaTiantian Yu0Tiantian Yu1Shan Gao2Fen Jin3Bingbing Yan4Wendong Wang5Zhongmin Wang6Dalian Medical University, Dalian, ChinaFemale Pelvic Floor Urinary Reconstructive Center, Dalian Women and Children’s Medical Group, Dalian, ChinaFemale Pelvic Floor Urinary Reconstructive Center, Dalian Women and Children’s Medical Group, Dalian, ChinaFemale Pelvic Floor Urinary Reconstructive Center, Dalian Women and Children’s Medical Group, Dalian, ChinaFemale Pelvic Floor Urinary Reconstructive Center, Dalian Women and Children’s Medical Group, Dalian, ChinaDepartment of Engineering Mechanics, Dalian University of Technology, Dalian, ChinaFemale Pelvic Floor Urinary Reconstructive Center, Dalian Women and Children’s Medical Group, Dalian, ChinaIntroductionEmerging evidence suggests that the vaginal microbiota is closely associated with cervical cancer. However, little is known about the relationships among the vaginal microbiota, vaginal metabolites, and cervical lesion progression in women undergoing cervical dysplasia.MethodsIn this study, to understand vaginal microbiota signatures and vaginal metabolite changes in women with cervical lesions of different grades and cancer, individuals with normal or cervical dysplasia were recruited and divided into healthy controls (HC) group, low-grade squamous intraepithelial lesions (LSIL) group, high-grade squamous intraepithelial lesions (HSIL) group, and cervical cancer (CC) group. Vaginal secretion samples were collected for 16S rRNA gene sequencing, liquid chromatography coupled with mass spectrometry (LC–MS)-based metabolomics, and integrated analysis.ResultsThe results demonstrated that bacterial richness and diversity were greater in the CC group than the other three groups. Additionally, Lactobacillus was found to be negatively associated with bacterial diversity and bacterial metabolic functions, which increased with the degree of cervical lesions and cancer. Metabolomic analysis revealed that distinct metabolites were enriched in these metabolite pathways, including tryptophan metabolism, retinol metabolism, glutathione metabolism, alanine, aspartate, and glutamate metabolism, as well as citrate cycle (TCA cycle). Correlation analysis revealed positive associations between CC group-decreased Lactobacillus abundance and CC group-decreased metabolites. Lactobacillus iners was both negative to nadB and kynU genes, the predicted abundance of which was significantly higher in the CC group. The linear regression model showed that the combination of the vaginal microbiota and vaginal metabolites has good diagnostic performance for cervical cancer.DiscussionOur results indicated a clear difference in the vaginal microbiota and vaginal metabolites of women with cervical dysplasia. Specifically altered bacteria and metabolites were closely associated with the degree of cervical lesions and cancer, indicating the potential of the vaginal microbiota and vaginal metabolites as modifiable factors and therapeutic targets for preventing cervical cancer.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1457216/fullcervical cancercervical lesions progressionvaginal microbiotavaginal metabolitescorrelation analysis |
| spellingShingle | Tiantian Yu Tiantian Yu Shan Gao Fen Jin Bingbing Yan Wendong Wang Zhongmin Wang Characteristics of the vaginal microbiota and vaginal metabolites in women with cervical dysplasia Frontiers in Cellular and Infection Microbiology cervical cancer cervical lesions progression vaginal microbiota vaginal metabolites correlation analysis |
| title | Characteristics of the vaginal microbiota and vaginal metabolites in women with cervical dysplasia |
| title_full | Characteristics of the vaginal microbiota and vaginal metabolites in women with cervical dysplasia |
| title_fullStr | Characteristics of the vaginal microbiota and vaginal metabolites in women with cervical dysplasia |
| title_full_unstemmed | Characteristics of the vaginal microbiota and vaginal metabolites in women with cervical dysplasia |
| title_short | Characteristics of the vaginal microbiota and vaginal metabolites in women with cervical dysplasia |
| title_sort | characteristics of the vaginal microbiota and vaginal metabolites in women with cervical dysplasia |
| topic | cervical cancer cervical lesions progression vaginal microbiota vaginal metabolites correlation analysis |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1457216/full |
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