Exploring the significant genetic diversity of Iranian isolates of Leishmania RNA virus 2 using whole genome sequence analysis
Abstract Background Our work presents the whole genome sequence and phylogenetic analysis of five Leishmania RNA virus 2 (LRV2) isolates obtained from patients with cutaneous leishmaniasis (CL) in Iran. Methods The whole genome sequencing of LRV2 was performed using a primer walking approach. The re...
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| Language: | English |
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BMC
2024-12-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-024-10194-6 |
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| author | Reza Saberi Homa Hajjaran Mahdi Fakhar Zahra Mirabedini Mehdi Mohebali |
| author_facet | Reza Saberi Homa Hajjaran Mahdi Fakhar Zahra Mirabedini Mehdi Mohebali |
| author_sort | Reza Saberi |
| collection | DOAJ |
| description | Abstract Background Our work presents the whole genome sequence and phylogenetic analysis of five Leishmania RNA virus 2 (LRV2) isolates obtained from patients with cutaneous leishmaniasis (CL) in Iran. Methods The whole genome sequencing of LRV2 was performed using a primer walking approach. The resulting sequences were analyzed for genetic and haplotype diversity, highlighting their independent evolution and significant genetic divergence. Results The whole genome sequence of the current LRV2 showed high genetic and haplotype diversity. The study also revealed the existence of three distinct clades of LRV2, with the LRV2 sequences infecting L. major, L. aethiopica, and sauroleishmania belonging to separate lineages. These lineages have seemingly evolved independently, as the geographic distribution of their flagellate hosts does not overlap with the Leishmania species. The divergence between these three clades is attributed to considerable antiquity, leading to genetic modifications within the viruses residing in them and resulting in structural differences in their genome. Conclusions These findings contribute to our understanding of the genetic diversity and evolution of LRVs, providing valuable insights into their role in Leishmania infections. Further investigations are needed to understand the significance of these polymorphic sites and their potential impact on viral characteristics and disease outcomes. |
| format | Article |
| id | doaj-art-2d808475c33b4abdb2c90b6b8a767e6d |
| institution | DOAJ |
| issn | 1471-2334 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
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| series | BMC Infectious Diseases |
| spelling | doaj-art-2d808475c33b4abdb2c90b6b8a767e6d2025-08-20T02:39:51ZengBMCBMC Infectious Diseases1471-23342024-12-0124111110.1186/s12879-024-10194-6Exploring the significant genetic diversity of Iranian isolates of Leishmania RNA virus 2 using whole genome sequence analysisReza Saberi0Homa Hajjaran1Mahdi Fakhar2Zahra Mirabedini3Mehdi Mohebali4Toxoplasmosis Research Center, Communicable Diseases Institute, School of Medicine, Mazandaran University of Medical SciencesDepartment of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical SciencesToxoplasmosis Research Center, Communicable Diseases Institute, School of Medicine, Mazandaran University of Medical SciencesDepartment of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical SciencesDepartment of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical SciencesAbstract Background Our work presents the whole genome sequence and phylogenetic analysis of five Leishmania RNA virus 2 (LRV2) isolates obtained from patients with cutaneous leishmaniasis (CL) in Iran. Methods The whole genome sequencing of LRV2 was performed using a primer walking approach. The resulting sequences were analyzed for genetic and haplotype diversity, highlighting their independent evolution and significant genetic divergence. Results The whole genome sequence of the current LRV2 showed high genetic and haplotype diversity. The study also revealed the existence of three distinct clades of LRV2, with the LRV2 sequences infecting L. major, L. aethiopica, and sauroleishmania belonging to separate lineages. These lineages have seemingly evolved independently, as the geographic distribution of their flagellate hosts does not overlap with the Leishmania species. The divergence between these three clades is attributed to considerable antiquity, leading to genetic modifications within the viruses residing in them and resulting in structural differences in their genome. Conclusions These findings contribute to our understanding of the genetic diversity and evolution of LRVs, providing valuable insights into their role in Leishmania infections. Further investigations are needed to understand the significance of these polymorphic sites and their potential impact on viral characteristics and disease outcomes.https://doi.org/10.1186/s12879-024-10194-6Cutaneous leishmaniasisGenetic diversityLeishmania RNA virus 2Primer walkingWhole genome sequence |
| spellingShingle | Reza Saberi Homa Hajjaran Mahdi Fakhar Zahra Mirabedini Mehdi Mohebali Exploring the significant genetic diversity of Iranian isolates of Leishmania RNA virus 2 using whole genome sequence analysis BMC Infectious Diseases Cutaneous leishmaniasis Genetic diversity Leishmania RNA virus 2 Primer walking Whole genome sequence |
| title | Exploring the significant genetic diversity of Iranian isolates of Leishmania RNA virus 2 using whole genome sequence analysis |
| title_full | Exploring the significant genetic diversity of Iranian isolates of Leishmania RNA virus 2 using whole genome sequence analysis |
| title_fullStr | Exploring the significant genetic diversity of Iranian isolates of Leishmania RNA virus 2 using whole genome sequence analysis |
| title_full_unstemmed | Exploring the significant genetic diversity of Iranian isolates of Leishmania RNA virus 2 using whole genome sequence analysis |
| title_short | Exploring the significant genetic diversity of Iranian isolates of Leishmania RNA virus 2 using whole genome sequence analysis |
| title_sort | exploring the significant genetic diversity of iranian isolates of leishmania rna virus 2 using whole genome sequence analysis |
| topic | Cutaneous leishmaniasis Genetic diversity Leishmania RNA virus 2 Primer walking Whole genome sequence |
| url | https://doi.org/10.1186/s12879-024-10194-6 |
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