Short-Chain Fatty Acid Utilization in <i>Cyberlindnera jadinii</i> for Single-Cell Protein and Odd-Chain Fatty Acid Production
In view of the growing global need for sustainable protein sources, this study explores the utilization of short-chain fatty acids into single-cell protein using the non-conventional yeast <i>Cyberlindnera jadinii</i>. Short-chain fatty acids can be sustainably produced via anaerobic dig...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/7/1558 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849409502357487616 |
|---|---|
| author | Christian Hermansen Rowanne Siao Gi Gi Chua Mikko Ru Xuan Lee Aaron Thong Melanie Weingarten Nic Lindley Eric Charles Peterson |
| author_facet | Christian Hermansen Rowanne Siao Gi Gi Chua Mikko Ru Xuan Lee Aaron Thong Melanie Weingarten Nic Lindley Eric Charles Peterson |
| author_sort | Christian Hermansen |
| collection | DOAJ |
| description | In view of the growing global need for sustainable protein sources, this study explores the utilization of short-chain fatty acids into single-cell protein using the non-conventional yeast <i>Cyberlindnera jadinii</i>. Short-chain fatty acids can be sustainably produced via anaerobic digestion of organic waste, presenting a promising fermentation substrate for a circular bioeconomy. <i>Cyberlindnera jadinii</i> is demonstrated to be capable of growing on acetate, propionate and butyrate as both a carbon and energy source without strong inhibition. Bioprocess development was conducted in stirred tank bioreactors, where a fed-batch pH-stat bioprocess led to improved efficiency without substrate inhibition. The highest titer of 31.3 ± 1.0 g/L, rate of 0.67 ± 0.02 g/L/h and yield of 0.36 ± 0.01 g/g was achieved with propionate. The resulting biomass contained 41.3% crude protein, and 17.3% crude lipids with 81% unsaturated fatty acids. In contrast to acetate and butyrate, propionate as a substrate led to accumulation of 37% odd-chain fatty acids with titer, rate and yield of 1.74 ± 0.06 g/L, 0.037 ± 0.001 g/L/h and 0.020 ± 0.001 g/g. These findings confirm that short-chain fatty acids are viable fermentation substrates not only for single-cell protein, but also unsaturated and odd-chain fatty acid production with <i>Cyberlindnera jadinii</i>. |
| format | Article |
| id | doaj-art-2d6b012d2e1b418cb59070e89cded848 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-2d6b012d2e1b418cb59070e89cded8482025-08-20T03:35:28ZengMDPI AGMicroorganisms2076-26072025-07-01137155810.3390/microorganisms13071558Short-Chain Fatty Acid Utilization in <i>Cyberlindnera jadinii</i> for Single-Cell Protein and Odd-Chain Fatty Acid ProductionChristian Hermansen0Rowanne Siao1Gi Gi Chua2Mikko Ru Xuan Lee3Aaron Thong4Melanie Weingarten5Nic Lindley6Eric Charles Peterson7Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology, and Research (A*STAR), 31 Biopolis Way, Nanos, Singapore 138669, SingaporeSingapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology, and Research (A*STAR), 31 Biopolis Way, Nanos, Singapore 138669, SingaporeSingapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology, and Research (A*STAR), 31 Biopolis Way, Nanos, Singapore 138669, SingaporeSingapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology, and Research (A*STAR), 31 Biopolis Way, Nanos, Singapore 138669, SingaporeSingapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology, and Research (A*STAR), 31 Biopolis Way, Nanos, Singapore 138669, SingaporeSingapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology, and Research (A*STAR), 31 Biopolis Way, Nanos, Singapore 138669, SingaporeSingapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology, and Research (A*STAR), 31 Biopolis Way, Nanos, Singapore 138669, SingaporeSingapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology, and Research (A*STAR), 31 Biopolis Way, Nanos, Singapore 138669, SingaporeIn view of the growing global need for sustainable protein sources, this study explores the utilization of short-chain fatty acids into single-cell protein using the non-conventional yeast <i>Cyberlindnera jadinii</i>. Short-chain fatty acids can be sustainably produced via anaerobic digestion of organic waste, presenting a promising fermentation substrate for a circular bioeconomy. <i>Cyberlindnera jadinii</i> is demonstrated to be capable of growing on acetate, propionate and butyrate as both a carbon and energy source without strong inhibition. Bioprocess development was conducted in stirred tank bioreactors, where a fed-batch pH-stat bioprocess led to improved efficiency without substrate inhibition. The highest titer of 31.3 ± 1.0 g/L, rate of 0.67 ± 0.02 g/L/h and yield of 0.36 ± 0.01 g/g was achieved with propionate. The resulting biomass contained 41.3% crude protein, and 17.3% crude lipids with 81% unsaturated fatty acids. In contrast to acetate and butyrate, propionate as a substrate led to accumulation of 37% odd-chain fatty acids with titer, rate and yield of 1.74 ± 0.06 g/L, 0.037 ± 0.001 g/L/h and 0.020 ± 0.001 g/g. These findings confirm that short-chain fatty acids are viable fermentation substrates not only for single-cell protein, but also unsaturated and odd-chain fatty acid production with <i>Cyberlindnera jadinii</i>.https://www.mdpi.com/2076-2607/13/7/1558short-chain fatty acidnon-conventional yeast<i>Cyberlindnera jadinii</i>bioprocess developmentsingle-cell proteinsingle-cell oil |
| spellingShingle | Christian Hermansen Rowanne Siao Gi Gi Chua Mikko Ru Xuan Lee Aaron Thong Melanie Weingarten Nic Lindley Eric Charles Peterson Short-Chain Fatty Acid Utilization in <i>Cyberlindnera jadinii</i> for Single-Cell Protein and Odd-Chain Fatty Acid Production Microorganisms short-chain fatty acid non-conventional yeast <i>Cyberlindnera jadinii</i> bioprocess development single-cell protein single-cell oil |
| title | Short-Chain Fatty Acid Utilization in <i>Cyberlindnera jadinii</i> for Single-Cell Protein and Odd-Chain Fatty Acid Production |
| title_full | Short-Chain Fatty Acid Utilization in <i>Cyberlindnera jadinii</i> for Single-Cell Protein and Odd-Chain Fatty Acid Production |
| title_fullStr | Short-Chain Fatty Acid Utilization in <i>Cyberlindnera jadinii</i> for Single-Cell Protein and Odd-Chain Fatty Acid Production |
| title_full_unstemmed | Short-Chain Fatty Acid Utilization in <i>Cyberlindnera jadinii</i> for Single-Cell Protein and Odd-Chain Fatty Acid Production |
| title_short | Short-Chain Fatty Acid Utilization in <i>Cyberlindnera jadinii</i> for Single-Cell Protein and Odd-Chain Fatty Acid Production |
| title_sort | short chain fatty acid utilization in i cyberlindnera jadinii i for single cell protein and odd chain fatty acid production |
| topic | short-chain fatty acid non-conventional yeast <i>Cyberlindnera jadinii</i> bioprocess development single-cell protein single-cell oil |
| url | https://www.mdpi.com/2076-2607/13/7/1558 |
| work_keys_str_mv | AT christianhermansen shortchainfattyacidutilizationinicyberlindnerajadiniiiforsinglecellproteinandoddchainfattyacidproduction AT rowannesiao shortchainfattyacidutilizationinicyberlindnerajadiniiiforsinglecellproteinandoddchainfattyacidproduction AT gigichua shortchainfattyacidutilizationinicyberlindnerajadiniiiforsinglecellproteinandoddchainfattyacidproduction AT mikkoruxuanlee shortchainfattyacidutilizationinicyberlindnerajadiniiiforsinglecellproteinandoddchainfattyacidproduction AT aaronthong shortchainfattyacidutilizationinicyberlindnerajadiniiiforsinglecellproteinandoddchainfattyacidproduction AT melanieweingarten shortchainfattyacidutilizationinicyberlindnerajadiniiiforsinglecellproteinandoddchainfattyacidproduction AT niclindley shortchainfattyacidutilizationinicyberlindnerajadiniiiforsinglecellproteinandoddchainfattyacidproduction AT ericcharlespeterson shortchainfattyacidutilizationinicyberlindnerajadiniiiforsinglecellproteinandoddchainfattyacidproduction |