Advanced glycation end products are biomolecular biomarkers for proliferative diabetic retinopathy

The present study examines the role of advanced glycation end products (AGEs) as biomolecular biomarkers for proliferative diabetic retinopathy (PDR). A cross-sectional study with a total of 80 consecutive cases of type 2 diabetes mellitus (DM) was divided into no retinopathy (NoDR) (n = 20), non-pr...

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Main Authors: Nibha Mishra, Sandeep Saxena, Rajendra K. Shukla, Anshul Tiwari
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2025-06-01
Series:Indian Journal of Ophthalmology
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Online Access:https://journals.lww.com/10.4103/IJO.IJO_2115_24
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author Nibha Mishra
Sandeep Saxena
Rajendra K. Shukla
Anshul Tiwari
author_facet Nibha Mishra
Sandeep Saxena
Rajendra K. Shukla
Anshul Tiwari
author_sort Nibha Mishra
collection DOAJ
description The present study examines the role of advanced glycation end products (AGEs) as biomolecular biomarkers for proliferative diabetic retinopathy (PDR). A cross-sectional study with a total of 80 consecutive cases of type 2 diabetes mellitus (DM) was divided into no retinopathy (NoDR) (n = 20), non-proliferative diabetic retinopathy (NPDR) (n = 20), PDR (n = 20), and controls (n = 20). AGEs were assessed by assay of N-carboxymethyl-lysine (Nε-CML). Nε- CML was evaluated using receiver operating characteristic curve (ROC) curve analysis, and the area under curve (AUC) was determined. The mean levels of Nε-CML were 31.3 ± 21.2 ng/mL, 73.9 ± 35.0 ng/mL, 91.2 ± 66.7 ng/mL, and 132.0 ± 84.0 ng/mL in control, NoDR, NPDR, and PDR, respectively. On analysis of variance (ANOVA), the Ne-CML level was significantly different between the study groups (control, NoDR, NPDR, and PDR) (P < 0.001). Best-corrected visual acuity decreased with disease progression and increased levels of Ne-CML (P < 0.001). On AUC analysis, the following values were obtained NoDR = 0.67, NPDR = 0.69, and PDR = 0.84, showing that AGEs are the most sensitive biomarkers for PDR in DR. AGEs serve as biomolecular biomarkers for PDR and can indicate disease conversion from NPDR to PDR. They are new-age tools of translational medicine.
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spelling doaj-art-2d67496a10e94070abb10d82deb847a32025-08-20T03:29:45ZengWolters Kluwer Medknow PublicationsIndian Journal of Ophthalmology0301-47381998-36892025-06-0173690691110.4103/IJO.IJO_2115_24Advanced glycation end products are biomolecular biomarkers for proliferative diabetic retinopathyNibha MishraSandeep SaxenaRajendra K. ShuklaAnshul TiwariThe present study examines the role of advanced glycation end products (AGEs) as biomolecular biomarkers for proliferative diabetic retinopathy (PDR). A cross-sectional study with a total of 80 consecutive cases of type 2 diabetes mellitus (DM) was divided into no retinopathy (NoDR) (n = 20), non-proliferative diabetic retinopathy (NPDR) (n = 20), PDR (n = 20), and controls (n = 20). AGEs were assessed by assay of N-carboxymethyl-lysine (Nε-CML). Nε- CML was evaluated using receiver operating characteristic curve (ROC) curve analysis, and the area under curve (AUC) was determined. The mean levels of Nε-CML were 31.3 ± 21.2 ng/mL, 73.9 ± 35.0 ng/mL, 91.2 ± 66.7 ng/mL, and 132.0 ± 84.0 ng/mL in control, NoDR, NPDR, and PDR, respectively. On analysis of variance (ANOVA), the Ne-CML level was significantly different between the study groups (control, NoDR, NPDR, and PDR) (P < 0.001). Best-corrected visual acuity decreased with disease progression and increased levels of Ne-CML (P < 0.001). On AUC analysis, the following values were obtained NoDR = 0.67, NPDR = 0.69, and PDR = 0.84, showing that AGEs are the most sensitive biomarkers for PDR in DR. AGEs serve as biomolecular biomarkers for PDR and can indicate disease conversion from NPDR to PDR. They are new-age tools of translational medicine.https://journals.lww.com/10.4103/IJO.IJO_2115_24advanced glycation end productsbiomarkerdiabetes mellitusdiabetic retinopathynε – carboxymethyl lysine
spellingShingle Nibha Mishra
Sandeep Saxena
Rajendra K. Shukla
Anshul Tiwari
Advanced glycation end products are biomolecular biomarkers for proliferative diabetic retinopathy
Indian Journal of Ophthalmology
advanced glycation end products
biomarker
diabetes mellitus
diabetic retinopathy
nε – carboxymethyl lysine
title Advanced glycation end products are biomolecular biomarkers for proliferative diabetic retinopathy
title_full Advanced glycation end products are biomolecular biomarkers for proliferative diabetic retinopathy
title_fullStr Advanced glycation end products are biomolecular biomarkers for proliferative diabetic retinopathy
title_full_unstemmed Advanced glycation end products are biomolecular biomarkers for proliferative diabetic retinopathy
title_short Advanced glycation end products are biomolecular biomarkers for proliferative diabetic retinopathy
title_sort advanced glycation end products are biomolecular biomarkers for proliferative diabetic retinopathy
topic advanced glycation end products
biomarker
diabetes mellitus
diabetic retinopathy
nε – carboxymethyl lysine
url https://journals.lww.com/10.4103/IJO.IJO_2115_24
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AT rajendrakshukla advancedglycationendproductsarebiomolecularbiomarkersforproliferativediabeticretinopathy
AT anshultiwari advancedglycationendproductsarebiomolecularbiomarkersforproliferativediabeticretinopathy