Cytokines profiles in cervical mucosa in patients with cervical high-risk human papillomavirus infection
Introduction: The purpose of this study was to detect the expression of local cytokines in cervical mucosa between patients with transient and persistent HR-HPV infection with or without CIN. Methodology: A total of 150 patients who were diagnosed as HR-HPV infection in Tianjin Central Hospital...
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The Journal of Infection in Developing Countries
2021-05-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/12147 |
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| author | De-Lai Long Hua-Lin Song Peng-Peng Qu |
| author_facet | De-Lai Long Hua-Lin Song Peng-Peng Qu |
| author_sort | De-Lai Long |
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Introduction: The purpose of this study was to detect the expression of local cytokines in cervical mucosa between patients with transient and persistent HR-HPV infection with or without CIN.
Methodology: A total of 150 patients who were diagnosed as HR-HPV infection in Tianjin Central Hospital of Obstetrics and Gynecology from January 2016 to December 2016 were included in this study. The expression levels of 9 cytokines in 150 patients with HR-HPV infection, including interleukin (IL)-1β, IL-4, IL-6, IL-8, IL-10, IL-12, IL-12p70, IL-21, interferon (IFN)-γ and tumor necrosis factor (TNF)-α, were simultaneously measured by using a multiplex immunoassay. Moreover, HR-HPV genotype was performed by using pyrosequencing. The association between cytokines and HPV genotype was also investigated.
Results: There was a statistically significant difference in IL-1β level between patients with HPV transient infection and HPV persistent infection (p = 0.041). There were statistically significant differences in the levels of IL-1β, IL-10, IL-21 and TNF-α between patients with low grade squamous intraepithelial lesion (LSIL) and high grade squamous intraepithelial lesion (HSIL) (p = 0.011, p = 0.008, p = 0.046 and p = 0.019, respectively).
Conclusions: Pro-inflammatory cytokines, IL-1β and TNF-α, and Th2 type cytokines, IL-10 and IL-21, became stronger in cervical mucosa with the progression of CIN. IL-1β may be advantageous for HR-HPV persistent infection.
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| format | Article |
| id | doaj-art-2d63a4cc26ff499380c049ee47d46c5c |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2021-05-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-2d63a4cc26ff499380c049ee47d46c5c2025-08-20T02:27:22ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802021-05-01150510.3855/jidc.12147Cytokines profiles in cervical mucosa in patients with cervical high-risk human papillomavirus infectionDe-Lai Long0Hua-Lin Song1Peng-Peng Qu2Clinical College of Central Gynecology and Obstetrics, Tianjin Medical University, Tianjin, ChinaDepartment of Gynecology and Obstetrics, Fuyang People’s Hospital, Anhui Province, ChinaDepartment of Gynecological Oncology, Tianjin Central Hospital of Gynecology and Obstetrics, Tianjin, China Introduction: The purpose of this study was to detect the expression of local cytokines in cervical mucosa between patients with transient and persistent HR-HPV infection with or without CIN. Methodology: A total of 150 patients who were diagnosed as HR-HPV infection in Tianjin Central Hospital of Obstetrics and Gynecology from January 2016 to December 2016 were included in this study. The expression levels of 9 cytokines in 150 patients with HR-HPV infection, including interleukin (IL)-1β, IL-4, IL-6, IL-8, IL-10, IL-12, IL-12p70, IL-21, interferon (IFN)-γ and tumor necrosis factor (TNF)-α, were simultaneously measured by using a multiplex immunoassay. Moreover, HR-HPV genotype was performed by using pyrosequencing. The association between cytokines and HPV genotype was also investigated. Results: There was a statistically significant difference in IL-1β level between patients with HPV transient infection and HPV persistent infection (p = 0.041). There were statistically significant differences in the levels of IL-1β, IL-10, IL-21 and TNF-α between patients with low grade squamous intraepithelial lesion (LSIL) and high grade squamous intraepithelial lesion (HSIL) (p = 0.011, p = 0.008, p = 0.046 and p = 0.019, respectively). Conclusions: Pro-inflammatory cytokines, IL-1β and TNF-α, and Th2 type cytokines, IL-10 and IL-21, became stronger in cervical mucosa with the progression of CIN. IL-1β may be advantageous for HR-HPV persistent infection. https://jidc.org/index.php/journal/article/view/12147cytokineHR-HPVimmune environmentpersistent HPV infectioncervical mucosa |
| spellingShingle | De-Lai Long Hua-Lin Song Peng-Peng Qu Cytokines profiles in cervical mucosa in patients with cervical high-risk human papillomavirus infection Journal of Infection in Developing Countries cytokine HR-HPV immune environment persistent HPV infection cervical mucosa |
| title | Cytokines profiles in cervical mucosa in patients with cervical high-risk human papillomavirus infection |
| title_full | Cytokines profiles in cervical mucosa in patients with cervical high-risk human papillomavirus infection |
| title_fullStr | Cytokines profiles in cervical mucosa in patients with cervical high-risk human papillomavirus infection |
| title_full_unstemmed | Cytokines profiles in cervical mucosa in patients with cervical high-risk human papillomavirus infection |
| title_short | Cytokines profiles in cervical mucosa in patients with cervical high-risk human papillomavirus infection |
| title_sort | cytokines profiles in cervical mucosa in patients with cervical high risk human papillomavirus infection |
| topic | cytokine HR-HPV immune environment persistent HPV infection cervical mucosa |
| url | https://jidc.org/index.php/journal/article/view/12147 |
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