Simultaneous recording of neuronal discharge and calcium activity reveals claustrum-cortex neurosynchrony under anesthesia
Neural information transmission between deep brain nuclei and the cortex is essential for brain function. Currently, high-resolution simultaneous detection of neural information between the deep brain nuclei and the large-scale cortex still poses challenges. We have developed the microelectrode arra...
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| Language: | English |
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KeAi Communications Co. Ltd.
2025-01-01
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| Series: | Fundamental Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266732582400030X |
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| author | Penghui Fan Rujin Zhang Guihua Xiao Yilin Song Chaowei Zhuang Lekang Yuan Fan Mo Botao Lu Zhaojie Xu Yiding Wang Jinping Luo Mixia Wang Weidong Mi Jiangbei Cao Qionghai Dai Xinxia Cai |
| author_facet | Penghui Fan Rujin Zhang Guihua Xiao Yilin Song Chaowei Zhuang Lekang Yuan Fan Mo Botao Lu Zhaojie Xu Yiding Wang Jinping Luo Mixia Wang Weidong Mi Jiangbei Cao Qionghai Dai Xinxia Cai |
| author_sort | Penghui Fan |
| collection | DOAJ |
| description | Neural information transmission between deep brain nuclei and the cortex is essential for brain function. Currently, high-resolution simultaneous detection of neural information between the deep brain nuclei and the large-scale cortex still poses challenges. We have developed the microelectrode arrays based on the Micro-Electro-Mechanical System technology, and modified the electrode surface with nanomaterials to improve the electrode performance. This study combined microelectrode arrays and extended-field-of-view microscopy to achieve simultaneous recording of claustrum (CLA) electrophysiology and wide-field cortical calcium imaging at single-cell resolution. This work investigated the synchronous changes of neural information in CLA and cortex of mice during the whole process from wakefulness to anesthesia and then to wakefulness, and summarized the characteristics of the CLA electrophysiology and cortical calcium signaling under different inhalation anesthesia concentrations. We found the synergy between microscopic spike and local field potential of CLA neurons under deep anesthesia, and the law that high inhalation anesthesia concentration enhanced the synchronization between neurons in CLA and cortex. The combination of microelectrode arrays and extended-field-of-view microscopy also gives a new method for synchronous detection of multimodal and multi-brain region neural information. |
| format | Article |
| id | doaj-art-2d5fceb64d0440e98b64216b8dae6de6 |
| institution | Kabale University |
| issn | 2667-3258 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | KeAi Communications Co. Ltd. |
| record_format | Article |
| series | Fundamental Research |
| spelling | doaj-art-2d5fceb64d0440e98b64216b8dae6de62025-01-29T05:02:35ZengKeAi Communications Co. Ltd.Fundamental Research2667-32582025-01-015193102Simultaneous recording of neuronal discharge and calcium activity reveals claustrum-cortex neurosynchrony under anesthesiaPenghui Fan0Rujin Zhang1Guihua Xiao2Yilin Song3Chaowei Zhuang4Lekang Yuan5Fan Mo6Botao Lu7Zhaojie Xu8Yiding Wang9Jinping Luo10Mixia Wang11Weidong Mi12Jiangbei Cao13Qionghai Dai14Xinxia Cai15State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaDepartment of Anesthesiology, The First Medical Center, Chinese PLA General Hospital, Beijing 100853, ChinaDepartment of Automation, Tsinghua University, Beijing 100084, ChinaState Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaDepartment of Automation, Tsinghua University, Beijing 100084, ChinaTsinghua-Berkeley Shenzhen Institute, Tsinghua University, Shenzhen 518055, ChinaState Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaDepartment of Anesthesiology, The First Medical Center, Chinese PLA General Hospital, Beijing 100853, ChinaDepartment of Anesthesiology, The First Medical Center, Chinese PLA General Hospital, Beijing 100853, China; Corresponding authors.Department of Automation, Tsinghua University, Beijing 100084, China; Corresponding authors.State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China; Corresponding authors.Neural information transmission between deep brain nuclei and the cortex is essential for brain function. Currently, high-resolution simultaneous detection of neural information between the deep brain nuclei and the large-scale cortex still poses challenges. We have developed the microelectrode arrays based on the Micro-Electro-Mechanical System technology, and modified the electrode surface with nanomaterials to improve the electrode performance. This study combined microelectrode arrays and extended-field-of-view microscopy to achieve simultaneous recording of claustrum (CLA) electrophysiology and wide-field cortical calcium imaging at single-cell resolution. This work investigated the synchronous changes of neural information in CLA and cortex of mice during the whole process from wakefulness to anesthesia and then to wakefulness, and summarized the characteristics of the CLA electrophysiology and cortical calcium signaling under different inhalation anesthesia concentrations. We found the synergy between microscopic spike and local field potential of CLA neurons under deep anesthesia, and the law that high inhalation anesthesia concentration enhanced the synchronization between neurons in CLA and cortex. The combination of microelectrode arrays and extended-field-of-view microscopy also gives a new method for synchronous detection of multimodal and multi-brain region neural information.http://www.sciencedirect.com/science/article/pii/S266732582400030XMicroelectrode arrayElectrophysiologyNeurosynchronyAnesthesiaCalcium imaging |
| spellingShingle | Penghui Fan Rujin Zhang Guihua Xiao Yilin Song Chaowei Zhuang Lekang Yuan Fan Mo Botao Lu Zhaojie Xu Yiding Wang Jinping Luo Mixia Wang Weidong Mi Jiangbei Cao Qionghai Dai Xinxia Cai Simultaneous recording of neuronal discharge and calcium activity reveals claustrum-cortex neurosynchrony under anesthesia Fundamental Research Microelectrode array Electrophysiology Neurosynchrony Anesthesia Calcium imaging |
| title | Simultaneous recording of neuronal discharge and calcium activity reveals claustrum-cortex neurosynchrony under anesthesia |
| title_full | Simultaneous recording of neuronal discharge and calcium activity reveals claustrum-cortex neurosynchrony under anesthesia |
| title_fullStr | Simultaneous recording of neuronal discharge and calcium activity reveals claustrum-cortex neurosynchrony under anesthesia |
| title_full_unstemmed | Simultaneous recording of neuronal discharge and calcium activity reveals claustrum-cortex neurosynchrony under anesthesia |
| title_short | Simultaneous recording of neuronal discharge and calcium activity reveals claustrum-cortex neurosynchrony under anesthesia |
| title_sort | simultaneous recording of neuronal discharge and calcium activity reveals claustrum cortex neurosynchrony under anesthesia |
| topic | Microelectrode array Electrophysiology Neurosynchrony Anesthesia Calcium imaging |
| url | http://www.sciencedirect.com/science/article/pii/S266732582400030X |
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