<i>Paracoccus broussonetiae</i> subsp. <i>drimophilus</i> subsp. nov., a Novel Subspecies Salt-Tolerant Endophytic Bacterium from Maize Root in Hunan
In an investigation exploring endophytic microbiota from agricultural crops, an aerobic, non-motile, Gram-negative, coccobacillus-shaped bacterial isolate, designated as strain NGMCC 1.201697<sup>T</sup>, was isolated from maize roots in Hunan Province, China. Phylogenetic analysis based...
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2025-02-01
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| author | Xue Li Chi Zhou Ming Li Qingzhuang Zhang Lei Su Xin Li |
| author_facet | Xue Li Chi Zhou Ming Li Qingzhuang Zhang Lei Su Xin Li |
| author_sort | Xue Li |
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| description | In an investigation exploring endophytic microbiota from agricultural crops, an aerobic, non-motile, Gram-negative, coccobacillus-shaped bacterial isolate, designated as strain NGMCC 1.201697<sup>T</sup>, was isolated from maize roots in Hunan Province, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NGMCC 1.201697<sup>T</sup> belonged to the genus <i>Paracoccus</i>, showing the highest sequence similarity to <i>Paracoccus broussonetiae</i> CPCC 101403<sup>T</sup> (99.86%). The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) were 98.57% and 87.90% between the novel isolate and its closest phylogenetic relative. However, phenotypic characterization further differentiated the isolate from <i>P. broussonetiae</i> CPCC 101403<sup>T</sup>. The isolate showed enhanced environmental tolerance adaptability (growth in 0–8% NaCl and 4–37 °C), unique enzymatic activities (esterase C4, β-glucosidase, L-proline arylamidase, and β-galactosidase), and expanded metabolic capabilities (D-mannitol, D-cellobiose, saccharose, and so on). The major polar lipids consisted of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylglycerol (PG), two unidentified glycolipids (GLs) and four unidentified phospholipids (PLs). The predominant respiratory quinone was ubiquinone-10, and the major fatty acid was summed feature 8 (C18:1 ω7c, 69.42%). The DNA G + C content was 64.49 mol%. Based on results of these analyses, strain NGMCC 1.201697<sup>T</sup> represents a novel subspecies of <i>Paracoccus broussonetiae</i>, for which the name <i>Paracoccus broussonetiae</i> subsp. drimophilus subsp. nov. is proposed. The type-strain is NGMCC 1.201697<sup>T</sup> (=CGMCC 1.61958<sup>T</sup> =JCM 37104<sup>T</sup>). |
| format | Article |
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| spelling | doaj-art-2d59ae8fd7864ff4a567d50cd7ec58cf2025-08-20T01:48:57ZengMDPI AGLife2075-17292025-02-0115335410.3390/life15030354<i>Paracoccus broussonetiae</i> subsp. <i>drimophilus</i> subsp. nov., a Novel Subspecies Salt-Tolerant Endophytic Bacterium from Maize Root in HunanXue Li0Chi Zhou1Ming Li2Qingzhuang Zhang3Lei Su4Xin Li5National Human Diseases Animal Model Resource Center, Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), Beijing 100021, ChinaHunan Institute of Microbiology, Changsha 410009, ChinaInstitute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaHunan Institute of Microbiology, Changsha 410009, ChinaNational Human Diseases Animal Model Resource Center, Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), Beijing 100021, ChinaHunan Institute of Microbiology, Changsha 410009, ChinaIn an investigation exploring endophytic microbiota from agricultural crops, an aerobic, non-motile, Gram-negative, coccobacillus-shaped bacterial isolate, designated as strain NGMCC 1.201697<sup>T</sup>, was isolated from maize roots in Hunan Province, China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NGMCC 1.201697<sup>T</sup> belonged to the genus <i>Paracoccus</i>, showing the highest sequence similarity to <i>Paracoccus broussonetiae</i> CPCC 101403<sup>T</sup> (99.86%). The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) were 98.57% and 87.90% between the novel isolate and its closest phylogenetic relative. However, phenotypic characterization further differentiated the isolate from <i>P. broussonetiae</i> CPCC 101403<sup>T</sup>. The isolate showed enhanced environmental tolerance adaptability (growth in 0–8% NaCl and 4–37 °C), unique enzymatic activities (esterase C4, β-glucosidase, L-proline arylamidase, and β-galactosidase), and expanded metabolic capabilities (D-mannitol, D-cellobiose, saccharose, and so on). The major polar lipids consisted of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylglycerol (PG), two unidentified glycolipids (GLs) and four unidentified phospholipids (PLs). The predominant respiratory quinone was ubiquinone-10, and the major fatty acid was summed feature 8 (C18:1 ω7c, 69.42%). The DNA G + C content was 64.49 mol%. Based on results of these analyses, strain NGMCC 1.201697<sup>T</sup> represents a novel subspecies of <i>Paracoccus broussonetiae</i>, for which the name <i>Paracoccus broussonetiae</i> subsp. drimophilus subsp. nov. is proposed. The type-strain is NGMCC 1.201697<sup>T</sup> (=CGMCC 1.61958<sup>T</sup> =JCM 37104<sup>T</sup>).https://www.mdpi.com/2075-1729/15/3/354endophytic bacteriumpolyphasic taxonomysalt-tolerant bacterium |
| spellingShingle | Xue Li Chi Zhou Ming Li Qingzhuang Zhang Lei Su Xin Li <i>Paracoccus broussonetiae</i> subsp. <i>drimophilus</i> subsp. nov., a Novel Subspecies Salt-Tolerant Endophytic Bacterium from Maize Root in Hunan Life endophytic bacterium polyphasic taxonomy salt-tolerant bacterium |
| title | <i>Paracoccus broussonetiae</i> subsp. <i>drimophilus</i> subsp. nov., a Novel Subspecies Salt-Tolerant Endophytic Bacterium from Maize Root in Hunan |
| title_full | <i>Paracoccus broussonetiae</i> subsp. <i>drimophilus</i> subsp. nov., a Novel Subspecies Salt-Tolerant Endophytic Bacterium from Maize Root in Hunan |
| title_fullStr | <i>Paracoccus broussonetiae</i> subsp. <i>drimophilus</i> subsp. nov., a Novel Subspecies Salt-Tolerant Endophytic Bacterium from Maize Root in Hunan |
| title_full_unstemmed | <i>Paracoccus broussonetiae</i> subsp. <i>drimophilus</i> subsp. nov., a Novel Subspecies Salt-Tolerant Endophytic Bacterium from Maize Root in Hunan |
| title_short | <i>Paracoccus broussonetiae</i> subsp. <i>drimophilus</i> subsp. nov., a Novel Subspecies Salt-Tolerant Endophytic Bacterium from Maize Root in Hunan |
| title_sort | i paracoccus broussonetiae i subsp i drimophilus i subsp nov a novel subspecies salt tolerant endophytic bacterium from maize root in hunan |
| topic | endophytic bacterium polyphasic taxonomy salt-tolerant bacterium |
| url | https://www.mdpi.com/2075-1729/15/3/354 |
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