Identification of Bed Bugs from Comoros, Using Morphological, Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry, and Molecular Biology Tools, and the Detection of Associated Bacteria
After virtually disappearing from domestic dwellings in the Western world at the end of the Second World War, bed bugs have re-emerged in recent years. Few studies, however, have been carried out on these insects in tropical islands. In this study, we focussed on describing bed bug specimens collect...
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MDPI AG
2025-02-01
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| Series: | Insects |
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| author | Saidou Ahamada M’madi Adama Zan Diarra Jean-Michel Bérenger Bouthaina Hasnaoui Philippe Parola |
| author_facet | Saidou Ahamada M’madi Adama Zan Diarra Jean-Michel Bérenger Bouthaina Hasnaoui Philippe Parola |
| author_sort | Saidou Ahamada M’madi |
| collection | DOAJ |
| description | After virtually disappearing from domestic dwellings in the Western world at the end of the Second World War, bed bugs have re-emerged in recent years. Few studies, however, have been carried out on these insects in tropical islands. In this study, we focussed on describing bed bug specimens collected from dwellings in a high-altitude village in Grande Comore, an island in the Comoros, in the Indian Ocean. We also aimed to detect the bacteria associated with them. Using MALDI-TOF MS coupled with molecular biology, we were able to confirm that the <i>C. hemipterus</i> species (the tropical bug) was the bug infesting these homes. Interestingly, the results also show that MALDI-TOF MS can differentiate between the developmental stages of bed bugs (immature and adult). Screening for bacteria was carried out using qPCR, regular PCR, and sequencing, with only <i>Wolbachia</i> DNA being found. Widespread surveys throughout the country are needed to ascertain the level of bed bug infestation, with a view to implementing appropriate control measures. |
| format | Article |
| id | doaj-art-2d553beaf6134f4a80ffb3245ec928eb |
| institution | DOAJ |
| issn | 2075-4450 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj-art-2d553beaf6134f4a80ffb3245ec928eb2025-08-20T02:45:00ZengMDPI AGInsects2075-44502025-02-0116214810.3390/insects16020148Identification of Bed Bugs from Comoros, Using Morphological, Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry, and Molecular Biology Tools, and the Detection of Associated BacteriaSaidou Ahamada M’madi0Adama Zan Diarra1Jean-Michel Bérenger2Bouthaina Hasnaoui3Philippe Parola4RITMES, Aix-Marseille Univ, SSA, 13005 Marseille, FranceRITMES, Aix-Marseille Univ, SSA, 13005 Marseille, FranceRITMES, Aix-Marseille Univ, SSA, 13005 Marseille, FranceRITMES, Aix-Marseille Univ, SSA, 13005 Marseille, FranceRITMES, Aix-Marseille Univ, SSA, 13005 Marseille, FranceAfter virtually disappearing from domestic dwellings in the Western world at the end of the Second World War, bed bugs have re-emerged in recent years. Few studies, however, have been carried out on these insects in tropical islands. In this study, we focussed on describing bed bug specimens collected from dwellings in a high-altitude village in Grande Comore, an island in the Comoros, in the Indian Ocean. We also aimed to detect the bacteria associated with them. Using MALDI-TOF MS coupled with molecular biology, we were able to confirm that the <i>C. hemipterus</i> species (the tropical bug) was the bug infesting these homes. Interestingly, the results also show that MALDI-TOF MS can differentiate between the developmental stages of bed bugs (immature and adult). Screening for bacteria was carried out using qPCR, regular PCR, and sequencing, with only <i>Wolbachia</i> DNA being found. Widespread surveys throughout the country are needed to ascertain the level of bed bug infestation, with a view to implementing appropriate control measures.https://www.mdpi.com/2075-4450/16/2/148bed bug<i>Cimex hemipterus</i>ComorosMALDI-TOF MS<i>Wolbachia</i> |
| spellingShingle | Saidou Ahamada M’madi Adama Zan Diarra Jean-Michel Bérenger Bouthaina Hasnaoui Philippe Parola Identification of Bed Bugs from Comoros, Using Morphological, Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry, and Molecular Biology Tools, and the Detection of Associated Bacteria Insects bed bug <i>Cimex hemipterus</i> Comoros MALDI-TOF MS <i>Wolbachia</i> |
| title | Identification of Bed Bugs from Comoros, Using Morphological, Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry, and Molecular Biology Tools, and the Detection of Associated Bacteria |
| title_full | Identification of Bed Bugs from Comoros, Using Morphological, Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry, and Molecular Biology Tools, and the Detection of Associated Bacteria |
| title_fullStr | Identification of Bed Bugs from Comoros, Using Morphological, Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry, and Molecular Biology Tools, and the Detection of Associated Bacteria |
| title_full_unstemmed | Identification of Bed Bugs from Comoros, Using Morphological, Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry, and Molecular Biology Tools, and the Detection of Associated Bacteria |
| title_short | Identification of Bed Bugs from Comoros, Using Morphological, Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry, and Molecular Biology Tools, and the Detection of Associated Bacteria |
| title_sort | identification of bed bugs from comoros using morphological matrix assisted laser desorption ionisation time of flight mass spectrometry and molecular biology tools and the detection of associated bacteria |
| topic | bed bug <i>Cimex hemipterus</i> Comoros MALDI-TOF MS <i>Wolbachia</i> |
| url | https://www.mdpi.com/2075-4450/16/2/148 |
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