Morphometric Changes in Male Reproductive Organs of Wistar Rats Following Combined Melatonin and Bisphenol-A Treatment
Bisphenol A (BPA) is a widespread endocrine disruptor known to cause reproductive toxicity. Melatonin has shown promise in ameliorating BPA-induced reproductive damage, but its effects on BPA-induced morphometric changes in reproductive tissues require further investigation. This study investigated...
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2025-03-01
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| Series: | Folia Veterinaria |
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| Online Access: | https://doi.org/10.2478/fv-2025-0003 |
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| author | Ajani Olumide Samuel Osunkoya Dideolu Akinniyi Olumide Odunayo |
| author_facet | Ajani Olumide Samuel Osunkoya Dideolu Akinniyi Olumide Odunayo |
| author_sort | Ajani Olumide Samuel |
| collection | DOAJ |
| description | Bisphenol A (BPA) is a widespread endocrine disruptor known to cause reproductive toxicity. Melatonin has shown promise in ameliorating BPA-induced reproductive damage, but its effects on BPA-induced morphometric changes in reproductive tissues require further investigation. This study investigated the effects of combined melatonin and BPA administration on morphometry of male reproductive organs in Wistar rats. Twenty-four adult male Wistar rats were divided into four groups: control, BPA-treated (10 mg/kg/day), melatonin-treated (10 mg/kg/day), and BPA + melatonin-treated. After 45 days, testes, epididymides, and spermatozoa were analyzed for morphometric parameters. BPA significantly reduced epididymal and testicular tubular density compared to control (p < 0.05). Melatonin treatment alone increased epididymal germinal epithelial height and decreased luminal diameter (p < 0.05). The combined BPA + melatonin treatment showed the lowest epididymal luminal diameter (p < 0.05) and highest testicular tubular density. Spermatozoa head diameter was significantly reduced in the melatonin group compared to the BPA group (p < 0.05). The combined treatment group showed a significant decrease in entire sperm length compared to all other groups (p < 0.05). Melatonin administration mitigated BPA-induced morphometric anomalies in male rats, primarily by increasing testicular and epididymal tubular density and modulating epididymal luminal diameter. This study demonstrated morphometric effects of BPA and melatonin on male reproductive systems, but the molecular mechanisms remain unexplained. Future research should explore biochemical pathways and long-term reproductive impacts. |
| format | Article |
| id | doaj-art-2d412a11d3d44aa380181e2ea73ac4cb |
| institution | DOAJ |
| issn | 2453-7837 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Sciendo |
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| series | Folia Veterinaria |
| spelling | doaj-art-2d412a11d3d44aa380181e2ea73ac4cb2025-08-20T03:10:04ZengSciendoFolia Veterinaria2453-78372025-03-01691172410.2478/fv-2025-0003Morphometric Changes in Male Reproductive Organs of Wistar Rats Following Combined Melatonin and Bisphenol-A TreatmentAjani Olumide Samuel0Osunkoya Dideolu1Akinniyi Olumide Odunayo2Department of Theriogenology, Faculty of Veterinary Medicine, University of Ibadan, NigeriaDepartment of Theriogenology, Faculty of Veterinary Medicine, University of Ibadan, NigeriaDepartment of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ibadan, NigeriaBisphenol A (BPA) is a widespread endocrine disruptor known to cause reproductive toxicity. Melatonin has shown promise in ameliorating BPA-induced reproductive damage, but its effects on BPA-induced morphometric changes in reproductive tissues require further investigation. This study investigated the effects of combined melatonin and BPA administration on morphometry of male reproductive organs in Wistar rats. Twenty-four adult male Wistar rats were divided into four groups: control, BPA-treated (10 mg/kg/day), melatonin-treated (10 mg/kg/day), and BPA + melatonin-treated. After 45 days, testes, epididymides, and spermatozoa were analyzed for morphometric parameters. BPA significantly reduced epididymal and testicular tubular density compared to control (p < 0.05). Melatonin treatment alone increased epididymal germinal epithelial height and decreased luminal diameter (p < 0.05). The combined BPA + melatonin treatment showed the lowest epididymal luminal diameter (p < 0.05) and highest testicular tubular density. Spermatozoa head diameter was significantly reduced in the melatonin group compared to the BPA group (p < 0.05). The combined treatment group showed a significant decrease in entire sperm length compared to all other groups (p < 0.05). Melatonin administration mitigated BPA-induced morphometric anomalies in male rats, primarily by increasing testicular and epididymal tubular density and modulating epididymal luminal diameter. This study demonstrated morphometric effects of BPA and melatonin on male reproductive systems, but the molecular mechanisms remain unexplained. Future research should explore biochemical pathways and long-term reproductive impacts.https://doi.org/10.2478/fv-2025-0003bisphenol amelatoninmorphometryreproductive toxicity |
| spellingShingle | Ajani Olumide Samuel Osunkoya Dideolu Akinniyi Olumide Odunayo Morphometric Changes in Male Reproductive Organs of Wistar Rats Following Combined Melatonin and Bisphenol-A Treatment Folia Veterinaria bisphenol a melatonin morphometry reproductive toxicity |
| title | Morphometric Changes in Male Reproductive Organs of Wistar Rats Following Combined Melatonin and Bisphenol-A Treatment |
| title_full | Morphometric Changes in Male Reproductive Organs of Wistar Rats Following Combined Melatonin and Bisphenol-A Treatment |
| title_fullStr | Morphometric Changes in Male Reproductive Organs of Wistar Rats Following Combined Melatonin and Bisphenol-A Treatment |
| title_full_unstemmed | Morphometric Changes in Male Reproductive Organs of Wistar Rats Following Combined Melatonin and Bisphenol-A Treatment |
| title_short | Morphometric Changes in Male Reproductive Organs of Wistar Rats Following Combined Melatonin and Bisphenol-A Treatment |
| title_sort | morphometric changes in male reproductive organs of wistar rats following combined melatonin and bisphenol a treatment |
| topic | bisphenol a melatonin morphometry reproductive toxicity |
| url | https://doi.org/10.2478/fv-2025-0003 |
| work_keys_str_mv | AT ajaniolumidesamuel morphometricchangesinmalereproductiveorgansofwistarratsfollowingcombinedmelatoninandbisphenolatreatment AT osunkoyadideolu morphometricchangesinmalereproductiveorgansofwistarratsfollowingcombinedmelatoninandbisphenolatreatment AT akinniyiolumideodunayo morphometricchangesinmalereproductiveorgansofwistarratsfollowingcombinedmelatoninandbisphenolatreatment |