UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit
Cornus mas L. is indigenous to Europe and parts of Asia. Although Cornus is widely considered to be an iridoid rich genera, only two iridoids have been previously found in this plant. The lack of information on taxonomically and biologically active iridoids prompted us to develop and optimize an ana...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/710972 |
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| author | Shixin Deng Brett J. West C. Jarakae Jensen |
| author_facet | Shixin Deng Brett J. West C. Jarakae Jensen |
| author_sort | Shixin Deng |
| collection | DOAJ |
| description | Cornus mas L. is indigenous to Europe and parts of Asia. Although Cornus is widely considered to be an iridoid rich genera, only two iridoids have been previously found in this plant. The lack of information on taxonomically and biologically active iridoids prompted us to develop and optimize an analytical method for characterization of additional phytochemicals in C. mas fruit. An ultra performance liquid chromatography (UPLC) coupled with photodiode array spectrophotometry (PDA) and electrospray time-of-flight mass spectrometry (ESI-TOF-MS) was employed and mass parameters were optimized. Identification was made by elucidating the mass spectral data and further confirmed by comparing retention times and UV spectra of target peaks with those of reference compounds. Primary DNA damage and antigenotoxicity tests in E. coli PQ37 were used to screen the iridoids for biological activity. As a result, ten phytochemicals were identified, including iridoids loganic acid, loganin, sweroside, and cornuside. Nine of these were reported for the first time from C. mas fruit. The iridoids did not induce SOS repair of DNA, indicating a lack of genotoxic activity in E. coli PQ37. However, loganin, sweroside, and cornuside did reduce the amount of DNA damage caused by 4-nitroquinoline 1-oxide, suggesting potential antigenotoxic activity. |
| format | Article |
| id | doaj-art-2d3a8366e7114584bda81b24ea295af4 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-2d3a8366e7114584bda81b24ea295af42025-02-03T01:09:58ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/710972710972UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas FruitShixin Deng0Brett J. West1C. Jarakae Jensen2Research and Development Department, Morinda Inc., 737 East, 1180 South, American Fork, UT 84003, USAResearch and Development Department, Morinda Inc., 737 East, 1180 South, American Fork, UT 84003, USAResearch and Development Department, Morinda Inc., 737 East, 1180 South, American Fork, UT 84003, USACornus mas L. is indigenous to Europe and parts of Asia. Although Cornus is widely considered to be an iridoid rich genera, only two iridoids have been previously found in this plant. The lack of information on taxonomically and biologically active iridoids prompted us to develop and optimize an analytical method for characterization of additional phytochemicals in C. mas fruit. An ultra performance liquid chromatography (UPLC) coupled with photodiode array spectrophotometry (PDA) and electrospray time-of-flight mass spectrometry (ESI-TOF-MS) was employed and mass parameters were optimized. Identification was made by elucidating the mass spectral data and further confirmed by comparing retention times and UV spectra of target peaks with those of reference compounds. Primary DNA damage and antigenotoxicity tests in E. coli PQ37 were used to screen the iridoids for biological activity. As a result, ten phytochemicals were identified, including iridoids loganic acid, loganin, sweroside, and cornuside. Nine of these were reported for the first time from C. mas fruit. The iridoids did not induce SOS repair of DNA, indicating a lack of genotoxic activity in E. coli PQ37. However, loganin, sweroside, and cornuside did reduce the amount of DNA damage caused by 4-nitroquinoline 1-oxide, suggesting potential antigenotoxic activity.http://dx.doi.org/10.1155/2013/710972 |
| spellingShingle | Shixin Deng Brett J. West C. Jarakae Jensen UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit Journal of Analytical Methods in Chemistry |
| title | UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit |
| title_full | UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit |
| title_fullStr | UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit |
| title_full_unstemmed | UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit |
| title_short | UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit |
| title_sort | uplc tof ms characterization and identification of bioactive iridoids in cornus mas fruit |
| url | http://dx.doi.org/10.1155/2013/710972 |
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